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Biomedical subjects

S J Lin

Publications and source records attributed to S J Lin.

At least 163 records · Page 9Linked to original sources
PubMed1990

Transendothelial macromolecular transport in the aorta of spontaneously hypertensive rats.

Leaky endothelial junctions occurring during cell turnover have been postulated to be a major pathway for enhanced lipoprotein transport across the vascular endothelial layer, which leads to the development of atherosclerosis. Because hypertension has been well documented as one of the major risk factors for atherosclerosis, we explored the possibility that hypertension accelerates atherogenesis by increasing the turnover of endothelial cells and hence the transendothelial macromolecular permeability. The investigations were performed on thoracic aortas of 10 male 3-4-month-old spontaneously hypertensive rats and eight male age-matched Wistar-Kyoto normotensive rats. In en face preparations of aortic specimens, mitotic endothelial cells were identified by hematoxylin nuclear staining; dying or dead endothelial cells containing cytoplasmic immunoglobulin G were detected by indirect immunoperoxidase technique; and endothelial leakage to Evans blue-albumin conjugate was visualized by fluorescence microscopy. The number of leaky foci per unit endothelial surface area in spontaneously hypertensive rats was found to be approximately three times that in Wistar-Kyoto control rats; the frequencies of both endothelial cell mitosis and death in spontaneously hypertensive rats were also approximately three times the corresponding values in Wistar-Kyoto rats. These findings indicate that hypertension in spontaneously hypertensive rats is accompanied by increased endothelial cell turnover and an attendant enhancement of permeability to macromolecules.

Animals↗
PubMed1990

[Studies on antigens of human lung adenocarcinoma with McAb LC-1].

The soluble antigens extracted from both human lung adenocarcinoma cell line SPC-A-1, and normal adult lung tissue with non-idet P-40 were subjected to 10% SDS-PAGE. The number of bands distinguishable by naked eyes of lung adenocarcinoma are 57, in which 4 bands are more significant. The bands of normal human lung tissue are 52, in which 2 bands are more significant. The molecular weights of these bands mainly are within 30 to 94 KD The thin layer chromatographs of these two antigenic extracts have shown that there is difference in their sugar content, but both of them shown little sialic acid. The Immunoblot pattern of McAb LC-1 reacted with the extracts of SPC-A-1 cells shows that all of 3 bands detected can be stained by alcian blue, indicating that they are glycoproteins. However, of them two bands, M. W. of 70 KD and 51 KD can also be stained by Sudan Black B, indicating that these two bands are glycolipoproteins. The ganglioside and neutral glycolipid can inhibit the binding of LC-1 with the extract of SPC-A-1 cells. The results indicate that the epitopes of SPC-A-1 cell extract reacted with McAb LC-1 are probably located in the polysaccharide.

Adenocarcinoma↗
PubMed1989

[McAb LC-1 against human lung cancer].

Recently Ge et al reported LAC-122, LAC-210 and LAC-163 McAbs against Human non-small cell lung cancer and LSC McAbs against human lung small cell carcinoma. The immunotoxin, composed of McAb LAC-122 conjugated with Ricin A chain has been reported to have the significant cytotoxic effect in vitro on lung adenocarcinoma cell line SPC-A-1 by Tan et al. The LAC-122 alone has no effect on this target cell in the presence of complement from human, rabbit or guinea pig. The tumor associated antigens of human lung cancer have been recognized for many years, but only few reports deal with the common antigens or common epitopes of the lung cancer. From one fusion, 20 hybrids had been observed, all of these culture supernatants could react with target cell by IF. One of them after 4 th cloning, immunoglobulin isotype of the monoclonal antibody thus far obtained belonged to IgM and named to LC-1. Table 1 showed the results of ABC staining of LC-1 with a variety of tumors, normal adult and fetal tissues. From 12 non-small cell lung cancers, including 7 lung adenocarcinoma, 2 lung squamous carcinoma, 3 lung giant cell carcinoma, only one adenocarcinoma gave negative staining. As for 6 small cell lung cancers, all of them showed the positive reaction. It could be also reacted with 11 other tumors.(ABSTRACT TRUNCATED AT 250 WORDS)

Antibodies, Monoclonal↗
PubMed1989

Human sperm chromosome analysis in primary infertility: a preliminary report.

Human sperm chromosomes can be analyzed using the zona-free hamster egg system. In order to familiarize ourselves with this new technique and to investigate the chromosome aberration frequency in fertile and possibly infertile men, we performed sperm chromosome analysis in 39 successful experiments. The subjects were divided into two groups. Group 1 was healthy men with proven fertility. Group 2 was husbands of couples with unexplained infertility. Semen samples were prepared freshly or preserved in TEST-yolk buffer for 12 to 72 hours. In group 1 using fresh samples, the mean penetration rate was 67.40 +/- 14.08% (mean +/- 2SD), the maturation index was 1.33 +/- 0.31. For samples treated with yolk buffer, the mean penetration rate was 91.34 +/- 7.42%, and the maturation index was 1.58 +/- 0.42. There was a statistically significant increase in penetration after yolk buffer treatment (p less than 0.001). In group 1 there were 89 haploid chromosomes, four were aneuploidy (4.5%), and three with structural aberrations (3.4%). In group 2, for fresh samples, the mean penetration rate was 71.34 +/- 27.96%, and the maturation index was 1.37 +/- 0.25. For samples treated with yolk buffer, the penetration rate was 86.33 +/- 25.18%, and maturation index 1.40 +/- 0.23. In group 2, there were 135 haploid chromosomes, 6 were aneuploidy (4.4%), and 4 with structural aberrations (3.0%). There was no statistically significant difference between group 1 and group 2 in the penetration rate, maturation index and frequency of chromosome abnormalities.

Chromosome Aberrations↗
PubMed1988

The role of arterial endothelial cell mitosis in macromolecular permeability.

The present experiments were performed on twelve male Wistar rats to study the quantitative, topographic correlation between transendothelial permeability of Evans Blue-albumin (EBA) conjugate and endothelial cell replication at the single-cell level. En face preparations of the thoracic aorta were examined by fluorescence microscopy. We found a high degree of correlation between endothelial cell mitosis and EBA leaky spots. Although endothelial cell mitosis is very rare in occurrence, nearly all junctions around the dividing cells were leaky (99%), in contrast to only 0.03% of the non-mitotic cells. In addition, electron microscopic observations showed that the junction around a dividing endothelial cell is leaky, whereas that around a dying cell is not. With the aid of our theoretical model, we were able to analyze the dynamics of macromolecular passage through leaky endothelial junctions. The duration of endothelial cell mitosis was estimated to be 67 min, which constituted 0.01% of the duration of the total cell cycle. The time-dependent change in junctional geometry during endothelial cell turnover leads to an inverse relationship between macromolecular size and duration of junctional leakage. For albumin the duration of leakiness across aortic endothelial cell is approximately 3.7 hr. The present findings lend support to our hypothesis that transiently open junctions surrounding the dividing endothelial cells provide the major pathway through which macromolecules enter the subendothelial space to result in lipid accumulation.

Animals↗
PubMed1988

Enhanced macromolecular permeability of aortic endothelial cells in association with mitosis.

Experiments were performed on thoracic aortae of 12 male Sprague-Dawley [corrected] rats to determine the statistical frequency of isolated leaks to Evans blue-albumin (EBA) conjugate at the level of individual cells and to assess the relationship of such leaks to the cell turnover process. Fluorescence microscopy was used to detect leakage of EBA around individual cells, and hematoxylin staining was used to identify cells in mitosis on the same specimens. Although endothelial cell mitosis is very rare in occurrence, 99% of the cells in the M phase were associated with EBA leakage. Since these dividing cells accounted for only one third of all cellular leakage sites, we concluded that significant leakage also occurred in other phases of the cell cycle, probably prior to and after the M phase, or during non-denuding desquamation.

Albumins↗
PubMed1987

An initial screening of serum lipids and fatty acid profiles of hypertensive and normotensive subjects.

Serum lipids and their acyl group profiles from a group of hypertensive patients with elevated systolic and diastolic pressure were compared with normotensive subjects of matched age, body weight and dietary habits. The level of serum triacylglycerols was elevated in the hypertensive subjects, but the cholesterol level remained normal. The acyl groups of serum triacylglycerols and cholesterylesters from hypertensive subjects indicated a higher proportion of the saturated fatty acids (16:0 and 18:0) and a lower proportion of linoleic acid (18:2) as compared to normal controls. There was no obvious change in the level and acyl group composition of serum phosphatidylcholine between the two groups. Since the hypertensive and normotensive subjects indicated similar dietary habits, the resulting differences in serum lipids reflected an abnormality in the lipolytic process in the hypertensive subjects.

Body Weight↗
PubMed1987

Further evidence for genetic heterogeneity of whistling face or Freeman-Sheldon syndrome in a Chinese family.

We report on 2 brothers with severe manifestations of the whistling-face/windmill-vane-hand syndrome (also called "craniocarpotarsal dystrophy"; Freeman-Sheldon syndrome); they were born to normal, nonconsanguineous parents. Our observations support the theory of causal heterogeneity of this syndrome and suggest existence of an autosomal or X-linked recessive form of this syndrome.

Abnormalities, Multiple↗
PubMed1987

Study of human chromosome V. The effects of thymidine concentration and timing on the expression of uridine-induced constitutive fragile sites.

The induction of fragile sites on human chromosomes has been demonstrated under various conditions that cause thymidylate stress, including exposure to uridine. In this study, we examined common fragile site expression by initially exposing peripheral lymphocytes to uridine, followed by repair of the fragile sites with media containing various concentrations of thymidine. Lymphocytes were cultured in medium 199 with 2 mM uridine. At 0.5, 1, 2, 3, 8, 10, 12, and 18 h before harvest, the uridine medium was removed and replaced by medium containing thymidine at various concentrations. Our results demonstrate that the effect of uridine on chromosome fragility can be reversed by low concentrations of thymidine (2 microM up to 200 microM) and the "rescuing" effect of thymidine can be achieved if the cells were treated prior to 2-3 h before harvest. No repair was found if thymidine was added to culture within 2 h prior to harvesting, suggesting that packing of chromosomes is also an important factor in the expression and repair of fragile sites.

Cells, Cultured↗
PubMed1986

Arterial hemodynamics in human hypertension.

Differences in aortic impedance between normotensives and hypertensives are not well characterized. We examined impedance in 8 normotensive and 11 hypertensive (mean 96.7 vs. 122.2 mmHg) age-matched, Chinese patients undergoing cardiac catheterization at rest, during nitroprusside, and handgrip exercise before and after beta blockade (propranolol). Hypertensives had higher resistance (2,295 vs. 1713 dyn-s/cm5), characteristic impedance (145.7 vs. 93.9 dyn-s/cm5), total external power (1,579 vs. 1174 mW), peripheral reflections (ratio of backward to forward wave components of 0.54 vs. 0.44), and first zero crossing of impedance phase angle (4.15 vs. 2.97 Hz). These abnormalities were eliminated with vasodilatation. Differences between groups were not further exacerbated when pressure was increased during handgrip exercise. Beta blockade further increased resistance and reflections. Thus, hemodynamic abnormalities of essential hypertension (increased resistance, reflections, and pulse wave velocity, and decreased compliance) are compatible with an increased vasomotor tone that is further unmasked during generalized beta blockade.

Adult↗
PubMed1986

[Fatal septicemia due to Chromobacterium violaceum].

A 2-year old boy was suffered from an infection caused by Chromobacterium violaceum. This is the first case of C. violaceum infection reported in Taiwan. The boy was treated with chloramphenicol and gentamicin but died after one month of illness due to the incooperation of patient's family. The causative agent was isolated from patient's blood and scar. Complete bacteriologic characteristics and antimicrobial susceptibility pattern of this isolate are reported.

Chromobacterium↗
PubMed1985

Activities of transport adenosine triphosphatases in erythrocyte membranes of healthy and hypertensive subjects.

The Na+, K+-ATPase activity in the erythrocyte membrane was measured in 25 untreated essential hypertensive patients and 25 age-matched healthy normotensive subjects. In addition, the Ca2+, Mg2+-ATPase activity was measured in 20 hypertensive and 25 age-matched normotensive subjects. The Na+, K+-ATPase activity of healthy Chinese measured in this study was similar to the data reported in a Dutch study. We therefore could not support a theory which speculated an ethnic influence on Na+, K+-ATPase activity. Both Na+, K+-ATPase and Ca2+, Mg2+-ATPase activities were slightly lower in hypertensive patients as compared with those in normotensive subjects, but the differences were not statistically significant. We concluded that the transport ATPase activities were not impaired in the erythrocyte membranes of hypertensive patients.

Adult↗