Biomedical subjects

S J Lockett

Publications and source records attributed to S J Lockett.

20 records · Page 2Linked to original sources

PubMed1987

The use of a multiwire camera for the detection of 3H labelled cDNA/mRNA hybrids.

We have investigated the use of a multiwire camera for the rapid direct quantitation of 3H labelled cDNA/mRNA hybrids. Growth hormone (GH) complementary DNA (cDNA) probes were labelled with either 3H or 32P and used in dot-blot hybridization assays of pituitary cytoplasmic samples. The results were assessed either by counting in the multiwire camera (3H) or by autoradiography and scanning densitometry (32P). Results obtained by both methods were comparable over a similar range of pituitary cytoplasmic dilutions. The use of a multiwire camera may have several advantages over conventional methods for the detection of radioisotopically labelled cDNA/mRNA hybrids and has great potential for use in this field of endocrine research.

Animals↗

PubMed1986

Preliminary assessment of a multiwire camera for quantitative autoradiography of tritium-labelled substances.

We have assessed a multiwire camera for the high speed, quantitative autoradiography of tritium-labelled substances in two-dimensional systems. Exposure times for 3H were typically 1000 times less than film-based methods, with a detection efficiency of 19 +/- 3% for those beta particles predicted to escape into the sensitive region from uniformly 3H-labelled plastic segments of 30 micron thickness. The spatial resolving power was calculated to be 0.4 mm full width half maximum (FWHM), the background count rate was 1 CPM cm-2 and the lowest activity monitored for a biochemical study was 0.15 Bq cm-2. The camera is expected to have wide applications for detecting and quantifying biological substances in a wide range of separation media.

Autoradiography↗