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S Joshi

Publications and source records attributed to S Joshi.

16 recordsLinked to original sources
PubMed1992

Oligomycin sensitivity-conferring protein (OSCP) of mitochondrial ATP synthase. The carboxyl-terminal region of OSCP is essential for the reconstitution of oligomycin-sensitive H(+)-ATPase.

Studies to establish the structure/function relationships of oligomycin sensitivity-conferring protein (OSCP) of mitochondrial ATP synthase were carried out using genetic engineering and biochemical approaches. A full-length cDNA clone encoding OSCP was isolated from a bovine heart cDNA library, and the mature form of OSCP was expressed in Escherichia coli using plasmid expression vector pKP1500. Recombinant OSCP was found to accumulate in the cytoplasmic inclusion bodies, by virtue of which the recombinant protein could be purified to greater than 85% purity by simple low speed centrifugation of cell lysates. Recombinant OSCP was found to be indistinguishable from OSCP isolated from mitochondria with respect to (i) apparent molecular mass on sodium dodecyl sulfate gel electrophoresis, (ii) immunological reactivity to anti-OSCP serum, (iii) biological activity in restoring oligomycin-sensitive ATPase and Pi-ATP exchange activities to OSCP-depleted ATP synthase complexes, and (iv) insensitivity of the biological activity to sulfhydryl-directed alkylating reagents. The amino-terminal sequence of the recombinant protein revealed that the initiating methionine was not removed by E. coli, although that apparently did not affect protein folding or its biological activity. Data on nested deletion mutations starting from the carboxyl terminus in OSCP demonstrated that, in each instance, the mutant form was expressed and the protein product was sequestered in cytoplasmic inclusion bodies, similar to the wild-type form. However, none of the variants, including the one in which only the last 10 residues were deleted, was able to restore cold-stable oligomycin-sensitive ATPase or Pi-ATP exchange activity in OSCP-depleted complexes. Taken together, these data suggest that amino acid residues 181-190 (or some of the residues in this region) in the OSCP sequence may be important for OSCP-F1 interactions.

Adenosine Triphosphatases↗
PubMed1992

Paranormal experiences in the general population.

The Dissociative Disorders Interview Schedule was administered to a random sample of 502 adults in the general population of Winnipeg, a midwestern Canadian city. Results showed that paranormal/extrasensory experiences were common in the general population. They were linked to a history of childhood trauma and to other dissociative symptom clusters. A factor analysis of the paranormal experiences identified three factors which together accounted for 44.0% of the combined variance of the scores. A model is proposed in which paranormal experiences are conceptualized as an aspect of normal dissociation. Like dissociation in general, paranormal experiences can be triggered by trauma, especially childhood physical or sexual abuse. Such experiences discriminate individuals with childhood trauma histories from those without at high levels of significance.

Acting Out↗
PubMed1992

Differential identification of Mycobacterium tuberculosis from various clinical specimens from Sassoon General Hospital, Pune.

A total of 619 clinical specimens from cases of pulmonary and extrapulmonary tuberculosis were processed by smear, culture and biochemical tests. Acid fast bacilli could be demonstrated in 93 samples (15.02%) by Z.N. staining method. Culture yielded positive growth in 95 samples (15.35%) M. tuberculosis human type was the most predominient pathogen obtained from 82 cultures (13.40%) M tuberculosis bovine type was isolated from 2 cases of ascitic fluids (0.32%). Atypical Mycobacteria were isolated from 11 samples (1.73%). 5 turned out to be M. scrofulacium, 4 were M. Kansasii, 1 was M. phlei and 1 was M. smegmatis.

Bacteriological Techniques↗
PubMed1992

Importance of periodic measurements in evaluating feeding programmes.

Attempts were made to show that periodic measurements are important for evaluating impact of feeding programmes as considerable "within variation" in increments exists which cannot be overlooked. From two socio-economic classes two kindergartens were selected one of which was implementing a feeding programme while the other was not. A total of 273 children were measured for weight and height, three times during the period of 7 months. Normal children showed significant gain in weight while malnourished children showed significant gain in height. The significance in weight (or height) increment at the end of the feeding programme did not necessarily mean that the significance could be obtained in each period even of equal time interval. Simple and cross correlations in increment revealed that weight increment in one period was significantly associated with height increment in the following period suggesting alternating priorities of the body for growth. Carefully planned longitudinal growth studies are therefore necessary for better understanding of growth process and thereby for proper evaluation of feeding programmes.

Body Height↗
PubMed1978

Kidney metabolism of acetaminophen and phenacetin.

The metabolism of acetaminophen and phenacetin by rabbit kidney slices was investigated. Phenacetin, like aspirin, inhibited [131I] Hippuran accumulation by the organic acid transport system. Phenacetin exhibited a dose-dependent inhibition with a K1 of 0.5 mM; acetaminophen at concentrations as high as 1 mM did not alter organic acid transport. [3H] acetaminophen slice: media ratios of approximately 1 or less suggested that acetaminophen entered cortex and outer and inner medullary slices by diffusion rather than active transport. Approximately 95% of the acetaminophen within the slices readily diffused out into the media. The chromatographic patterns of this material were similar to that of the incubation media. The acetaminophen remaining within the slices was acid-precipitable and not extractable with organic solvents. This covalent binding of acetaminophen was inhibited by glutathione. More acetaminophen was bound in the renal medullar than cortex. These data suggest that the renal metabolism of acetaminophen observed in this in vitro study may be related to the nephritis observed in analgesic abuse.

Acetaminophen↗
PubMed1975

[Emissions from combustion of indoor dust (author's transl)].

Combustion of dust is an old problem which has gained a new importance since the introduction of electric storage heaters for space heating. Physico-chemical changes caused by heating indoor dust in a model apparatus were investigated. Four dust samples of different origins were heated at three temperatures (200, 400, 600 degrees C) for three residence times (5, 15, 30 min). The higher the temperature, the higher the weight loss expressed as percentage (15 to 75%). The residence time had no effect on the loss. Three dust samples showed pH of about 7 before heated, while the fourth showed pH of about 10. After heating to 200 degrees C, the pH of one of the samples rose from 7 to 10. When heated to 400 and 600 degrees C, all the samples showed pH values between 10 and 11. The residence time had no effect. The pH of the gaseous reaction products did not show uniform pattern at 200 degrees C. At 400 degrees C, the measured pH was between 7.5 and 8.5, while at 600 degrees C no further change was observed. Changes in pH took place during the first fifteen minutes. Chemical analysis of the reaction products with help of detector tubes indicated that dust samples when heated emit carbon monoxide, carbon dioxide, oxides of nitrogen and ammonia.

Air↗
PubMedDate not supplied

Schneiderian symptoms and childhood trauma in the general population.

The Dissociative Disorders Interview Schedule (DDIS) was administered to a sample of 502 adults in the city of Winnipeg, Manitoba, Canada. Findings indicate that Schneiderian symptoms are highly related to childhood trauma and other dissociative symptoms clusters in the general population, as they are in clinical populations. Implications of the findings are discussed.

Adult↗