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S K Bose

Publications and source records attributed to S K Bose.

At least 73 records · Page 4Linked to original sources
PubMed1985

Functional characterization of constituent enzyme fractions of mycobacillin synthetase.

The enzyme fraction A, a constituent enzyme of the three-fraction enzyme mycobacillin synthetase, independently and sequentially activated five amino acids starting from L-proline, producing the pentapeptide Pro(Asp1,Glu1,Tyr1)Asp. The fractions B and C were unable to function independently. However, the fraction B synthesized the nonapeptide Pro(Asp3,Glu1,Tyr2,Ser1)Leu, sequentially activating the pentapeptide and next four amino acids, whereas the fraction C synthesized mycobacillin by the sequential activation of the nonapeptide and the remaining four amino acids. The pH optima of the above enzymes are almost identical (pH 7.8), but their Km values are a little different.

Adenosine Triphosphate↗
PubMed1985

Sensitized photo-oxidation of bovine alpha-crystallin: change in microenvironments of thiol groups.

Methylene blue- or riboflavin-sensitized photo-oxidation of alpha-crystallin caused a major change in the environment of thiol groups of the protein which could not be significantly prevented by inhibitors specific for active species of oxygen, 1O2, .OH, O.2, and H2O2. In contrast, the sensitizer N-formylkynurenine had no such effect. Sensitizer-induced change in the microenvironments of thiol groups was attributed to a conformational alteration, probably an unfolding of the protein.

Animals↗
PubMed1985

Translocation of mycobacillin synthetase in Bacillus subtilis.

The extracellular release of mycobacillin from Bacillus subtilis first occurred in the medium at the onset of stationary phase and continued at a high rate even after 6 days. Mycobacillin synthetase activity appeared earlier than late-exponential phase in the cytosol of producer cells and was not sedimentable even at 105 000 g. The activity then quickly reached the maximum late in the stationary phase. With further increase in the age of the culture, the activity gradually disappeared from the cytosol, to reappear concomitantly in the membrane in an insoluble particulate form, even in absence of protein synthesis. The membrane-bound synthetase activity was sedimentable at 10 000 g and was fairly active even after 5 days.

Bacillus subtilis↗
PubMed1985

Age-related changes in protein conformation in bovine lens crystallins.

In order to investigate the conformational changes associated with the aging process, circular dichroism (CD), absorption and fluorescence measurements of bovine lens crystallins isolated from the nucleus of old (cow) and young (calf) animals are reported. Results show considerable differences in spectroscopic parameters between the young and old alpha-crystallin; however, no such changes were observed for beta- and gamma-crystallins. Age-related changes include an increased absorption in near-u.v. and decreased intensity in the far-u.v. region; near-u.v. circular dichroism shows a considerable difference, whereas the dichroism in far-u.v. remains the same. The decrease in tryptophan fluorescence of old alpha-crystallin is of the same magnitude as is the increase in non-tryptophan fluorescence. The fluorescence of the sulfhydryl (SH) specific probe, 2-(4'-maleimidylanilino) naphthalene-6-sulfonate, indicates that accessible (to the probe) SH groups of cow alpha-crystallin are fewer than those of calf, and they are also in a more polar environment. This study demonstrates that, with aging, alpha-crystallin undergoes a change in the tertiary structure involving tryptophan, tyrosine and cysteine residues. This conformational change has been explained by the suggestion that a large portion of the protein unfolds during the aging process, resulting in a change in interaction properties between the aromatic amino acid residues and between the residues and the peptide backbone. The unfolding is also associated with the accessibility, reactivity and spatial arrangement of these residues, including the cysteine by which aggregation or cross-linking of the protein is likely to occur.

Absorption↗
PubMed1985

Accumulation of peptides by mycobacillin-negative mutants of Bacillus subtilis B3.

Thirteen mycobacillin-negative (My-) mutants of Bacillus subtilis B3 were isolated from an auxotrophically tagged mycobacillin producer organism. The wild-type producer, three feeble producers and three strictly My- mutants did not accumulate any ninhydrin-positive peptide in the culture medium while the remaining seven My- mutants did accumulate ten such peptides whose amino acid composition indicated that there might be only three different peptides. The N-terminal and C-terminal amino acid residues implicated one of these peptides as a pentapeptide intermediate in mycobacillin synthesis; this was further confirmed by its molecular weight and sequence. Studies on cell-free synthesis showed that only the enzyme system from the wild-type strain synthesized mycobacillin while the defective ones from all the My- mutants synthesized one and the same pentapeptide as found in the culture broth of some of the mutants. Further studies in which the enzymes responsible for mycobacillin synthesis by cell-free extracts were separated into three fractions, A, B and C, showed that seven of the mutants were defective in fraction B whereas the three other mutants had defects in both fractions B and C. Thus the pentapeptide Pro----Asp----Glu----Tyr----Asp appears to be implicated in mycobacillin biosynthesis.

Amino Acid Sequence↗
PubMed1985

Derepression of sporulation and synthesis of mycobacillin and dipicolinic acid by guanosine 3':5'-cyclic monophosphate under conditions of glucose repression in Bacillus subtilis.

Dibutyryl cyclic GMP, but not dibutyryl cyclic AMP, derepresses sporulation and synthesis of mycobacillin and dipicolinic acid under conditions of glucose repression in Bacillus subtilis strain B34. Neither of these compounds appears to affect sporulation and synthesis of mycobacillin and dipicolinic acid in this strain under normal physiological conditions. Mutants insensitive to glucose repression were indifferent to the addition of either of the nucleotides both in the presence and in the absence of glucose. A role for dibutyryl cyclic GMP in annulling the repressing effect of glucose on sporulation and on synthesis of mycobacillin and dipicolinic acid is thus indicated.

Antifungal Agents↗
PubMed1984

Standardization of parameters for the mycobacillin synthetase activity.

An effective method of preparation involving sonication was developed for cell-free mycobacillin synthetase from Bacillus subtilis. The enzyme showed optimum activity at a buffer concentration of 50 mM (Tris-HCl) and pH 7.5. ATP and Mg2+ which were essential for synthesis showed an optimum requirement at a ratio of 1:1. The synthetase was markedly inhibited by ADP whereas AMP was without any effect. ATP or ATP-generating system could not be replaced by GTP, UTP or CTP. Co2+ and Mn2+ could to some extent substitute Mg2+. Mercapto reagents inhibited the antibiotic synthesis. Exogenous addition of pantothenic acid had no effect.

Bacillus subtilis↗
PubMed1984

Mycoversilin, a new antifungal antibiotic. III. Mechanism of action on a filamentous fungus Trichophyton rubrum.

Mycoversilin is active against filamentous fungi, being specifically inhibitory to Trichophyton rubrum, minimum inhibitory concentration being 15 micrograms/ml. Mycoversilin inhibits sporulation to the extent of 28.5% even at the growth inhibitory concentration whereas inhibition of spore germination requires higher concentration. It has no effect on radial growth. Further it shows no action either on the release of UV absorbing materials or on the respiration of T. rubrum. However, the antibiotic inhibits in vivo synthesis of protein fairly strongly, DNA moderately and RNA slightly at the minimum inhibitory concentration. Cell-free protein synthesis is also strongly inhibited, the site of action being the inhibition of leucyl-tRNA formation by the antibiotic which has no action on leucine activation.

Antifungal Agents↗
PubMed1984

Mycoversilin, a new antifungal antibiotic. I. Fermentation, isolation and biological properties.

A new antifungal antibiotic, mycoversilin, was isolated from the culture filtrate of Aspergillus versicolor (N5)17 by repeated column chromatography and recrystallized from ethyl acetate as homogeneous fine needles. Maximum production took place in a medium containing 4% glucose and 1% peptone at pH 3.5, temperature 28 degrees C after 8-9 days of incubation under stationary condition. Mycoversilin is a narrow spectrum antibiotic with activity against filamentous fungi, particularly Trichophyton rubrum (MIC 15 micrograms/ml).

Antifungal Agents↗
PubMed1984

Mycoversilin, a new antifungal antibiotic. II. Structure elucidation.

The structure of a new antifungal antibiotic, mycoversilin, produced by Aspergillus versicolor (N5)17 was determined as I by various spectroscopic and chemical methods. Mycoversilin is a unique polynuclear aromatic compound having two methyl and six hydroxyl groups and two ether linkages. The acetyl derivative prepared was found to have no antifungal property.

Antifungal Agents↗
PubMed1983

Fractionation of the mycobacillin-synthesizing enzyme system.

The mycobacillin-synthesizing enzyme system was highly purified by fractionation at 30-55% (NH4)2SO4 saturation. The enzyme concentrate on Sephadex G-200 gel chromatography was resolved into three distinct fragments. Each of the fragments on further purification by DEAE-cellulose ion-exchange chromatography behaved as a single-component system, as clearly indicated by the sharpness of the peaks in the elution diagram. None of the fragments alone nor any two of them in all possible combinations possessed mycobacillin-synthesizing activity, which was restored only when the three fragments were used together in the test system.

Antifungal Agents↗
PubMed1983

Influence of lectins, hexoses, and neuraminidase on the association of purified elementary bodies of Chlamydia trachomatis UW-31 with HeLa cells.

Using highly purified elementary bodies of Chlamydia trachomatis UW-31 (serotype K), we found that HeLa 229 monolayer cultures bound more 32P-labeled chlamydiae after pretreatment with the lectin wheat germ agglutinin. The lectin, on the other hand, inhibited competitively when chlamydial association was assayed in the presence of polycations. The two effects of wheat germ agglutinin were abolished when N-acetylneuraminic acid (NeuNAc)- or N-acetylglucosamine (GlcNAc)-preincubated wheat germ agglutinin was used. Brief exposure of HeLa cells to neuraminidase abolished the ability to bind the elementary bodies, whether or not polycations were present. Furthermore, at 5 degrees C but not at 37 degrees C, NeuNAc, GlcNAc and N-acetylgalactosamine inhibited chlamydial association only in the absence of the polycation DEAE-dextran. The results suggest that NeuNAc residues on the plasma membrane are the principal, but not the only, receptors for this strain of C. trachomatis.

Acetylgalactosamine↗
PubMed1982

Purification of Chlamydia trachomatis lymphogranuloma venereum elementary bodies and their interaction with HeLa cells.

A procedure has been developed to yield infectious elementary bodies of the lymphogranuloma venereum strains LGV 434 and 404 of Chlamydia trachomatis, labelled during intracellular growth in HeLa 229 cells. The final preparation, obtained after velocity sedimentation of a polycarbonate membrane-filtered sample through a sucrose gradient, is free of host proteins and, more importantly, of chlamydial reticulate bodies. Using such purified preparations, it was found that the association of LGV 434 elementary bodies with HeLa 229 cultures was unaffected by the pretreatment of the host cells with a variety of lectins or with neuraminidases from Clostridium perfringens and Vibrio cholerae. The association was inhibited by dextran sulphate and by mild trypsin treatment of HeLa cultures. Treatment of purified elementary bodies with trypsin, chymotrypsin, neuraminidases and a variety of carbohydrates and lectins did not produce any change in the rate of association with HeLa cultures. Heat-inactivated elementary bodies were significantly less able to associate with the host cells.

Centrifugation, Density Gradient↗
PubMed1982

Molecular connectivity and antifungal activity. A quantitative structure-activity relationship study of substituted phenols against skin pathogens.

The plot of minimum inhibitory concentration (MIC) vs. 2Xv of twelve phenolic compounds delineates the congeners into three distinct classes: the most potent ortho compounds describe a parabola, para substituted compounds of intermediate potency lie in a line, while the least potent meta derivatives yield a scatter diagram. The same pattern is found for two skin pathogens, Trichophyton rubrum and Epidermophyton floccosum. From non-linear regression analysis 2Xv is found to be excellently correlated with observed MIC values of the most potent ortho compounds against both the test organisms. For both the organisms logP is found to be inferior to 2Xv in the correlation of MIC values. On the basis of regression equations the MIC values of certain ortho substituted phenols are calculated a priori, some of which are corroborated by findings reported in the literature.

Antifungal Agents↗