PubMed Health⌕ Search

Biomedical subjects

S K Mukherjee

Publications and source records attributed to S K Mukherjee.

At least 73 records · Page 4Linked to original sources
PubMed1985

Effect of epinephrine and norepinephrine on immuno-reactive insulin secretion from isolated islets of Langerhans.

Earlier studies have demonstrated the inhibitory nature of epinephrine and norepinephrine on pancreatic insulin release. The present study reports their effect on beta-cell IRI release in an isolated islet system. Results show that epinephrine and norepinephrine inhibit islet-IRI release at the 100-10 microM level. The alpha adrenergic blocker phentolamine (100 microM) but not the beta adrenergic blocker propranolol (100 microM) can reverse this catecholamine induced inhibition of islet-IRI release. This clearly suggests that epinephrine and norepinephrine inhibit insulin release via alpha-adrenergic pathway.

Animals↗
PubMed1985

Effects of SITS on urate transport by isolated, perfused snake renal tubules.

Effects of an inhibitor of membrane anion-exchange transport processes, 4-acetamido-4'-isothiocyano-2,2'-disulfonic stilbene (SITS), on urate transport by isolated, perfused snake (Thamnophis spp.) proximal renal tubules were studied. SITS (10(-4) mol/l) in the luminal perfusate had absolutely no effect on net urate secretion (Jneturate) or on net fluid absorption (JV). This observation is compatible with other data that give no support to the concept of a mediated transport step for urate from the cells to the lumen. SITS (10(-4) mol/l) in the bathing medium reversibly inhibited Jneturate without affecting JV. At the time of maximum inhibition of Jneturate, the concentration of urate in the cell water was increased and the apparent permeability of the luminal membrane to urate was decreased, but the urate efflux across the peritubular membrane and the apparent permeability of the peritubular membrane to urate were unchanged. There was no evidence of significant intracellular binding or trapping of urate. Although an increase in the initial rate of urate transport into the cells across the peritubular membrane could not be demonstrated conclusively in nonperfused tubules, the results still suggest that SITS in the bathing medium may inhibit Jneturate by inhibiting urate movement from the cells to the lumen while actually enhancing transport from the bathing medium into the cells.

4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfo↗
PubMed1984

Hypolipidemic effect of garlic and thyroid function.

Male Sprague-Dawley rats were maintained on cholesterol and garlic oil for 12 weeks. Cholesterol induced hyperlipidemia was controlled by garlic feeding. Garlic treatment did not alter the concentrations of circulating thyroid hormones and thyroidal uptake of radioiodine. The results indicate that the hypolipidemic effect of garlic is probably not mediated through the thyroid.

Animals↗
PubMed1984

In vivo mutagenic effect of methyldopa. 1. Dominant lethal test in male mice.

The dominant lethal test was used to evaluate the mutagenic potential of methyldopa, an antihypertensive drug, in male mice. In the series treated, animals were exposed to 480 or 960 mg/kg of the drug as a suspension in 1% gum acacia via the oral route. A separate group of males served as control. Following treatment each male of the control, as as well as the treated series, was mated to two untreated virgin females each week for a period of 6 consecutive weeks. All mated females were sacrificed on the 10th day of separation and their ovaries and uterine contents were examined. Corpora lutea, implantations and resorptions were recorded. The drug treatment did not impair the mating capacity and fertility. Pre- and post-implantation losses were assessed. Higher incidences of post-implantation losses and abortion, and lower incidences of total implantations particularly at higher dose levels clearly reveal a dominant lethal effect of the drug.

Abortion, Spontaneous↗
PubMed1982

Isolation of bacteria producing chloramphenicol acetyltransferase from soil and their characterization.

After screening 107 soil samples collected from different spots around Calcutta, 579 chloramphenicol resistant colonies were isolated. Out of these only 58 colonies could inactivate chloramphenicol in detectable amounts. By noting the production of inactivating factor, 5 high yielding strains were further characterized to species level. Three of them were Escherichia coli strains, the two others were Alcaligenes faecalis and Klebsiella pneumoniae strains. All strains inactivated chloramphenicol by acetylation, with the production of chloramphenicol acetyltransferase. Production of this latter enzyme was not inducible. Minimum inhibitory concentrations for these 5 strains were studied against 14 antimicrobial agents. All strains were found to be resistant to most antimicrobial agents, but sensitive to polymyxin B. The strain A. faecalis was also sensitive to carbenicillin but other four strains were resistant to this antibiotic.

Acetyltransferases↗
PubMed1981

Mechanisms of hemodynamic actions of furosemide: differentiation of vascular and renal effects on blood pressure in functionally anephric hypertensive patients.

To differentiate diuretic and direct cardiocirculatory properties of furosemide for elucidation of the vasodepressor mechanisms of action of the agent in the acute treatment of hypertension, the peripheral vascular effects of intravenous furosemide (3 mg/kg) on supine blood pressure (BP) and forearm hemodynamics in 11 functionally anephric hypertensive patients (creatinine clearance less than 2 ml/min) were studied. BP was recorded by sphygmomanometer and forearm hemodynamics were measured by strain gauge plethysmography. While diastolic BP decreased only 2.7 mm Hg at 30 minutes, forearm blood flow increased 55% (p less than 0.01) mediated by decreased peripheral vascular resistance of 30% (p less than 0.01) at 15 minutes which dissipated by 30 minutes. Systolic BP, indices of venous capacity, weight, hematocrit, serum electrolytes, and plasma renin activity were unchanged. No diuresis occurred. It is concluded that the early hypotensive effect of furosemide depends upon diuresis.

Adult↗
PubMed1981

Excision arthroplasty for tuberculous and pyogenic arthritis of the hip.

Thirty patients with chronic pyogenic or tuberculous arthritis of the hip treated by Girdlestone's excision arthroplasty were reviewed two to seven years after operation. There was marked or complete relief of pain in 29, control of infection in 27, squatting and sitting cross-legged was possible in 27, and 16 were able to stand on the operated limb. Overall results were good in 16, fair in nine, and poor in five. Tuberculous disease was not reactivated and the use of traction for 12 weeks and a weight-relieving caliper for 12 months after operation helped to reduce the shortening to an average of 3.8 centimetres. Excision arthroplasty is considered a sound operation to restore the ability to squat and sit cross-legged.

Adolescent↗