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S K Nishida

Publications and source records attributed to S K Nishida.

22 records · Page 2Linked to original sources
PubMed1986

Radioimmunoassay for IgG and IgM rheumatoid factors.

The present paper describes two solid phase radioimmunoassays for detecting IgG and IgM rheumatoid factors (RF). Basically, serum rheumatoid factors were bound to rabbit IgG previously adsorbed to wells of flexible microtiter plates and quantitated after the addition of 125I-Fab anti-human gamma Fd for the IgG-RF assay or 125I-IgG anti-mu chain for the IgM-RF assay. The IgG and IgM rheumatoid factor assays were tested on 56 and 30 control sera, respectively. The upper normal limits for these assays were taken for cpm test serum/cpm control pool as 1.00 (0.76 + 0.24; X + 2SD) and 1.39 (0.73 + 0.66; X + 2SD) for IgG-RF and IgM-RF, respectively. When applied to sera of patients with rheumatoid arthritis, IgM-rheumatoid factor was detected in 15 of 15 (100%) patients, and IgG-rheumatoid factor in 15 of 17 (88%). The radioimmunoassay results for both rheumatoid factors were significantly higher for these patients than for the normal control sera (P less than 0.01).

Animals↗
PubMedDate not supplied

[Retinol-binding protein urinary levels in a pediatric population: evolution according to age]

OBJECTIVE: The kidney of the child, mainly the newborn, is specially susceptible to ischemic and/or toxic injuries, which can affect the renal tubular function. Therefore, reliable and precocious markers of tubular dysfunction would be useful in Pediatrics. The urinary levels of retinol binding-protein (RBPu) has been used in this way. Our goal was to evaluate the RBPu behavior in childhood to establish a reference interval and verify possible differences according to the age. METHODS: We studied healthy children aged 1 month to 8 years and healthy term and preterm babies; in the last group we only included babies without hemodynamic repercussion. We collected isolated urine samples once for children above 1 month and periodically for those below this age. In this urine, besides the dipsticks test (Combur 9(R), Boehringer), RBPu and creatinine were measured. RESULTS: We observed that the RBPu levels in childhood do not have a normal distribution; after logarithm data transformation we were able to establish an interval of reference for children above one month of age: 3 to 269 micro g/L, mean of 27 micro g/ L. In newborns, we found a large variation of RBPu excretion. However, we noticed that the excretion decreases significantly, reaching the healthy adult levels at the end of the 1st month, for term as well as for preterm newborns. CONCLUSIONS: We suggest that RBPu can be used as a marker of proximal tubular dysfunction, since we could establish an interval of reference, and it can be repeated whenever necessary in the follow-up of this kind of injury, because it is not an invasive exam. Moreover, this study contributes to the knowledge of the tubular function development for showing that at the end of the 1st month of life the newborn's kidney is able to reabsorb RBPu as the healthy adult kidney is.

Journal Article↗
PubMedDate not supplied

[Serum beta 2 microglobulin (beta 2M) following renal transplantation].

Although there was an important improvement in graft and patient survival the last 10 years, graft rejection continues to be a major barrier to the success of renal transplantation. Identification of a laboratory test that could help to diagnose graft rejection would facilitate the management of renal transplanted patients. PURPOSE--To evaluate the utility of monitoring serum beta 2M in recently transplanted patients. METHODS--We daily determined serum beta 2M levels in 20 receptors of renal grafts (10 from living related and 10 from cadaveric donors) and compared them to their clinical and laboratory evolution. RESULTS--Eight patients who presented immediate good renal function following grafting and did not have rejection had a mean serum beta 2M of 3.7 mg/L on the 4th day post transplant. The sensitivity of the test for the diagnosis of acute rejection was 87.5%, but the specificity was only 46%. Patients who presented acute tubular necrosis (ATN) without rejection had a progressive decrease in their serum levels of beta 2M, while their serum creatinine changed as they were dialyzed. In contrast, patients with ATN and concomitance of acute rejection or CSA nephrotoxicity presented elevated beta 2M and creatinine serum levels. CONCLUSION--Daily monitoring of serum beta 2M does not improve the ability to diagnose acute rejection in patients with good renal function. However, serum beta 2M levels seemed to be useful in diagnosing acute rejection or CSA nephrotoxicity in patients with ATN.

Adolescent↗