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Biomedical subjects

S K Zhigletsova

Publications and source records attributed to S K Zhigletsova.

8 recordsLinked to original sources

[Initial stages of steel biocorrosion].

Initial stages of corrosion of mild steel induced by Klebsiela rhinoscleromatis BO2 were studied in various media. The effect of the microorganism was detected 8-10 h after inoculation. The number of viable cells were virtually unchanged within one month in all media, but the corrosive activity of the strain decreased. The corrosive activity of microorganisms can be determined by spectrophotometry even only after incubation for 24 h. At a low level of organic substrate, even strong colonization with microorganisms does not inevitably result in a significant damage to metals.

Biodegradation, Environmental↗

[Development and testing of "Ekosorb" biosorbent based on association of petroleum-oxidizing bacteria for purifying petroleum-polluted soils].

A biosorbent containing an association of oil-oxidizing bacteria as a main constituent was developed, in which Lessorb, a product of moss and wood thermal processing, was used as a carrier. Xeroprotectors preserving the cell viability and oil-oxidizing activity in the biosorbent on drying and after long-term storage were selected. The use of this biosorbent for cleaning oil-polluted sod-podzol soils showed a two-threefold cleanup rate acceleration at different pollution levels (8 and 24 l/m2), especially in the presence of a nitrogen-phosphate fertilizer. The biosorbent increased the populations of certain groups of soil microorganisms and the total soil biological activity.

Absorption↗

[Creation and use of a liquid substance based on association of petroleum-oxidizing bacteria].

An association of four bacterial strains with high oil-oxidizing and bioemulsifying activities, psychrophilicity, resistance to chemical pollutants, and lack of pathogenicity was selected from a collection of natural oil-oxidizing microorganisms. A new liquid preparation containing stabilizers and preservatives that maintain the cell viability and oil-oxidizing activity during long-term storage was developed. A field experiment in oil-polluted sod-podzol and clay sand soils demonstrated that this preparation accelerated the biodegradation of oil and its individual fractions, especially in the presence of mineral and organic fertilizers. Treatment of oil-polluted soil with this preparation and additives decreased the oil-induced suppression of certain groups of soil microflora.

Acinetobacter↗

[Development of biological methods of combating steel corrosion, induced by aerobic microorganisms].

Cocultivation of degrading microorganisms and their antagonists decreases the corrosion loss of carbon steel by 20 to 80%. It was found that a microorganism can either accelerate or inhibit corrosion, depending on the nutrient. The magnitude of the effect on corrosion depends on the ability of the microorganism to respond to changes in the nutrient-medium composition by releasing acidic or alkaline metabolites.

Bacteria, Aerobic↗

[Chemical-microbiological diagnosis of stress-corrosive damage to pipelines]].

Samples of soil, ground, electrolyte, corrosion products, and protective coating were taken after excavating pipelines. The depth of stress corrosion cracks of the pipe steel was mostly related to the numbers of sulfate-reducing and denitrifying bacteria. In certain types of soil, damage correlated with the number of acid-producing microorganisms and aerobic chemoorganotrophs (saprophytes). A correlation was found between the extent of stress-corrosion damage to pipelines and the contents of reduced iron, sulfides, and organic carbon in surrounding ground.

Bacteria↗

[Increase in the ecological danger upon the use of biocides for fighting corrosion induced by microorganisms].

Five synergistic combinations of biocides were found, among which the combination of kathon + copper sulfate was the most efficient against Serratia marcescens. Depending on the ratio of these biocides, the synergistic effect of this pair allowed 4-20-fold decreases in the effective concentrations. Combinations of biocides with salts (carbonates and phosphates) that facilitate passivation of steel were found, which considerably decreased the corrosion losses of mild steel in comparison to isolated treatment with biocides or salts. The data showed that biocides must be added to corrosion-prone systems simultaneously with the beginning of their exploitation. Otherwise, considerably excessive amounts of biocides or their synergistic compositions are needed.

Bacteria↗

[Direct quantitative method for evaluating the effect of biocides on Pseudomonas fluorescens in different culture media].

A method for quantitative evaluation of the effects of biocides is presented. The method was tested in experiments with Pseudomonas fluorescens grown on various agar nutrient media. The effective concentrations of biocides that decreased the maximum specific rate of the colony biomass growth (mu'm) were called S (suppressing) concentrations, and concentrations that decreased the number of colony-forming units (CFU) were taken as L (sublethal) concentrations. The efficiency of the reported approach was demonstrated in experiments with three biocides tested in four nutrient media. It was found that the biocide sensitivity of Pseudomonas fluorescens varied by a factor of 30, depending on the amount and the type of the nutrient substrate.

Anti-Bacterial Agents↗

[Microfungicid--a preparation based on trichoderma viride for plant diseases control].

A technology was designed for manufacturing a preparation based on Trichoderma viride Pers ex S.F. Gray that strongly suppresses the development of causative agents of certain plant diseases and displays a growth-stimulating activity. Cultivation of the strain in a liquid medium for 18-24 h produced up to 60 g dry biomass per liter nutrient medium. A marketable form created in this work conserves the activity of the mycelial preparation for six months. The preparation is compatible with insecticides (carbofos, vismetrin, talstar, and applaud) and certain fungicides (such as baitan). Tests performed with the liquid form of Mycofungicid (seeds were treated with this preparation at a dose of 20-30 g per metric ton before sowing) showed its high efficiency in protecting cereal crops from plant pathogens. The incidence of plant diseases decreased by 65%, and crop yields increased by 15-20%.

Fungicides, Industrial↗