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Biomedical subjects

S Kamiński

Publications and source records attributed to S Kamiński.

At least 19 recordsLinked to original sources
PubMed1993

A simple and powerful method for linkage analysis by amplification of DNA from single sperm cells.

Haplotypes of three bovine casein loci were analyzed as a way of determining genetic linkage. A total of 330 individual sperm cells from a triply heterozygous bull were selected by means of a new method involving fixing the sperm cells into low-melting-point agarose gels. The method is simple and very accurate with an efficiency close to 100% for picking sperm cells and producing amplifiable DNA templates, circumventing the complicated statistical analysis mandated by automated cell sorting. The DNA was amplified in a two-step polymerase chain reaction (PCR) to achieve the necessary high specificity of amplification. In the first reaction, primers flanking the polymorphic site at each locus were used. The PCR product from the first reaction was then reamplified in a second PCR using primers that create allele-specific restriction sites (ACRS) in the PCR products for two of the three loci, allowing the alleles to be determined by gel electrophoresis. No recombinants were found among the 330 single sperm cells analyzed, giving a lod score higher than 30 and proving a very strong linkage between bovine casein genes.

Animals↗
PubMed1988

Postoperative radiotherapy and radiotherapy combined with CCNU chemotherapy for treatment of brain gliomas.

A prospective, randomized trial evaluates the effects of two postoperative treatment regimens on survival in 198 adult patients with supratentorial gliomas. All patients were irradiated with 6,000 rads after possibly radical removal of tumors. CCNU administration in the doses of 100 mg/sq m of body surface every 6-8 weeks following surgery proved to have no significant effect on the survival of patients. The median survival time in patients receiving radiation therapy alone was 61 +/- 7 weeks, while in those receiving additional chemotherapy was 56 +/- 4 weeks. Tumor histological malignancy and patients age were found to be the only important prognostic factors, irrespective of the treatment modality.

Adolescent↗
PubMed1980

[Angiographic evaluation of the effectiveness of surgical treatment of intracranial aneurysm].

On the basis of control angiographies after surgical treatment of 59 intracranial aneurysms full effectiveness of aneurysm elimination from the circulation was assessed and found to be fully effective in 93.2% of cases. In 3 cases the aneurysmal sac was partially visualized, and in 2 cases the metal clip was found to have slipped off from the aneurysm neck. One of these patients was reoperated successfully. The investigations demonstrated in 17 cases (29%) presence of postoperative vasospasm, in 8 cases internal hydrocephalus following past bleeding and in 2 cases accumulation of cerebrospinal fluid in the subdural space. On the basis of literature reports and own experiences the authors stress the necessity of possibly early control angiography which should be accepted as a routine method. In case of internal hydrocephalus demonstration insertion of a ventriculoatrial shunt is suggested.

Adolescent↗
PubMed1980

[Results of surgical treatment of aneurysms of the anterior communicating artery].

The authors present the results of surgical treatment of 48 aneurysms situated on the anterior communicating artery operated upon by the classic and microsurgical methods. The latter method has made it possible to raise the proportion of very good and good results from 23% in the classic method to 70%, and to reduce the postoperative mortality in groups I and II of condition severity from 13.6% to 4%. Independently of the method used Korsakoff's syndrome was observed in 17% of cases. The high value of Yasargil's surgical approach is stressed.

Adolescent↗
PubMed1979

[Giant intracranial aneurysm].

The authors describe a case of a giant intracranial aneurysm of the left internal carotid artery in which signs of raised intracranial pressure predominated. In view of presence of internal hydrocephalus Holter's valve was implanted. The aneurysm was excluded from the circulation by trapping the afferent vessel. A good therapeutic result was achieved.

Adult↗
PubMedDate not supplied

[Diagnosis of cerebello-pontine angle tumors].

History and results of investigations in 34 patients with cerebello-pontine angle tumours are presented. Tumour was diagnosed in the first, audiological stage of the disease only in 4 patients. In 7 more patients additional symptoms of central nervous system focal disorders occurred. The remaining 23 patients were in the third stage of the disease with symptoms of increased intracranial pressure. The main reason for such a late diagnosis of the tumour was neglecting of the early audiological symptoms of the disease by patients and by general practitioners as well.

Adult↗
PubMedDate not supplied

[Fronto-temporo-sphenoidal craniotomy using the Yasargil method in operations of aneurysms and expanding lesions of basal areas of the brain].

The authors describe in detail the technique of fronto-temporo-sphenoidal craniotomy of Yasargil and stress its advantages in surgical treatment of aneurysms of the circle of Willis. This method was used in surgical treatment of 82 patients with intracranial aneurysms, expanding lesions situated in the vicinity of the sella and ala parva of the sphenoid bone, and with other intracranial processes, and very good conditions for operation were obtained. The authors believe that fronto-temporo-sphenoidal craniotomy is the most favourable approach for the management of aneurysms of the circulus arteriosus.

Circle of Willis↗