PubMed Health⌕ Search

Biomedical subjects

S Kanai

Publications and source records attributed to S Kanai.

13 recordsLinked to original sources
PubMed1979

Fibrin suspension as a substrate fop plasmin: determination and kinetics.

Fibrin polymers formed from fibrinogen with thrombin in the presence of EDTA were suspended in a medium containing glucose, arabic gum and imidazole-HCl buffer and were sonicated at 20 kHz for 20 min to make a suspension containing fibrin particles of small size. The fibrin suspension was used as a substrate of plasmin for determining the enzymic activity of plasmin and plasminogen activated with urokinase. The kinetic study on the reaction of the fibrin particles with plasmin in the presence and the absence of fibrinogen revealed that Km value of fibrin for plasmin is 4.2 x 10(-7) M and the Ki value of fibrinogen is 1.2 x 10(-5) M.

Electrophoresis, Disc↗
PubMed1979

Fibrin membrane endowed with biological function. IV. Formation of cross-links between fibrinogen (or fibrin) and ribonuclease by transglutaminase.

Transglutaminase from guinea pig liver catalyzed the formation of cross-links between fibrinogen (or fibrin) and ribonuclease. Using transglutaminase, immoblized ribonuclease was prepared by two separate methods: (1) fibrinogen-ribonuclease conjugates formed by transglutaminase were treated with thrombin to make fibrin membrane bound covalently to the enzyme; (2) fibrin polymer formed from fibrinogen with thrombin was covalently bound to ribonuclease by transglutaminase to make fibrin-ribonuclease conjugates.

Animals↗
PubMed1978

Difference in the effect of bucolome on the hepatic transport maximum of sulfobromophthalein and indocyanine green.

The effects of bucolome (BC, 1-cyclohexyl-5-n-butyl-2,4,6-trioxoperhydropyrimidine, a non-steroid antiinflammatory agent), a potent choleretic, on the maximal biliary excretion rates (Tm) of sulfobromophthalein (BSP) and indocyanine green (ICG) were investigated in rats. With continuous infusion of BSP or ICG, Tm and bile flow rate in control rats and rats given BC (40--100 min after 20 mg/100 g body weight i.p. injection) were compared. The BSP Tm was not significantly different in control and BC administered rats (BC rats), while the bile flow rate and endogenous bile salts excretion rate were significantly higher in BC rats. On the other hand, ICG Tm was significantly higher in BC rats, although ICG concentration in bile was significantly lower in BC rats. The rates of the rise in plasma concentration of the dyes were significantly lower in BC rats. However, the plasma concentration of ICG was significantly higher in BC rats at corresponding time intervals than in control rats throughout the experimental period. The difference in the effect of BC on the transport of these two dyes indicates variability in the regulatory mechanisms for hepatic anion transport.

Animals↗
PubMed1978

Faster determination of clottable fibrinogen in human plasma: an improved method and kinetic study.

Clottable fibrinogen in human plasma was determined by measuring spectrophotometrically the increase in turbidity with time due to the fibrinogen-fibrin conversion with thrombin. From the maximal absorbance, Amax, at 450 nm obtained 2 min or less after thrombin as added to plasma, we estimated the fibrinogen concentration in plasma of normal subjects and patients. Analysis of the rate of the absorbance increase yielded the Km value, 1.6 X 10(-5) mol/liter, which closely agrees with the Km of 1.2 X 10(-5) mol/liter obtained by analysis of the fibrinopeptides released from fibrinogen.

Fibrinogen↗
PubMed1977

Bile-salt-dependent and independent choleresis induced by bucolome in the rat.

Choleresis induced by bucolome (BC) (1-cyclohexyl-5-n-butyl-2,4,6-trioxoperhydropyrimidine) was studied in male Wistar rats. [14C]Erythritol and mannitol clearance studies indicated this choleresis to be of canalicular origin. In 1-h continuous bile collection studies, immediately after the interruption of enterohepatic circulation (acute interruption), both bile flow and bile salt excretion rates were significantly increased in rats administered BC. However, the bile salt excretion rate fell rather rapidly in BC-administered rats, while the bile flow rate was fairly constant during this 1-h period. Thus, unlike the situation in control rats, bile flow rate was not significantly correlated with the bile salt excretion rate in BC-administered rats. In rats that had an external bile fistula open for 16-20 h (chronic interruption of enterohepatic circulation) the bile flow rate was also significantly increased by BC administration, while the bile salt excretion rate was not changed after BC administration. It is suggested that BC induced bile-salt-independent choleresis in both experimental rat groups (acute and chronic interruption of enterohepatic circulation). In addition, BC appeared to increase the bile-salt-dependent fraction of bile in rats with acute interruption of enterohepatic circulation, possibly by mobilizing the bile salt pooled in the intestinal content and (or) intestinal wall.

Animals↗
PubMedDate not supplied

Increased biliary excretion of ouabain induced by bucolome in the rat.

1. Following the intravenous injection of 3H-ouabain (0.4 or 0.6 mg/100 g body weight) the plasma concentration and biliary excretion of ouabain were compared for control male Wistar rats and rats given bucolome (BC, 1-cyclohexyl-5-n-butyl-2,4,6-trioxoperhydroxypyrimidine, 20 mg/100 g) 40 min before the ouabain injection. In bucolome treated rats, the bile flow rate was 80-90% higher than in control rats and the biliary excretion rate of ouabain for the 40 min post-injection period was significantly higher in bucolome treated rat groups. The increase was due to an approximately two-fold increase in the excretion rate in the first 10 min period. On the other hand, plasma concentration of ouabain was significantly higher in bucolome treated rats compared with control rats at corresponding time intervals. Plasma volume as determined by 131I-labelled albumin dilution was not decreased in bucolome treated rats. 2. The results indicated that the significant increase in biliary excretion of ouabain administration was due to the enhancement of the hepatic transport and/or biliary excretion process and not due to an increase in hepatic uptake.

Animals↗