Biomedical subjects
Publications and source records attributed to S Kanta.
Five hundred soil samples were examined for Nocardia species in Patiala area by using paraffin bait technique. Forty (8 per cent) yielded Nocardia species. All the forty isolates identified as Nocardia asteroides were pathogenic to white mice.
Four hundred isolates of Gram Negative bacilli from different samples were tested by three tube method and conventional tests. An overall agreement between the two methods was 89.5%. Non-agreement was in respect of less stable tests like gas production and motility. This does not reflect adversely on the efficacy and suitability of the method. The three tube method is simple, rapid, economical and accurate, saving both time and material.
A total of 150 strains of E. coli isolated from faecal samples of normal population (Group I), hospital personnel (Group II) and hospitalised patients (Group III) were studied for the transfer of R-plasmids. Auto-transferable R-plasmids were observed in 72.0, 82.0 and 80.0 per cent of groups I, II and III respectively. 77.0 per cent strains were typable. 04 was the most common serotype found in groups I and II, 0134 in group III.
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Inorganic nitrogens sources like nitrate, nitrite enhanced sporulation and glycogen accumulation in Anabaena sp. but ammonium chloride neither influenced sporulation nor glycogen accumulation. Acetate and citrate also stimulated early sporulation and glycogen level was higher over nitrogen free control. Nitrogen and carbon sources in combination proved to be useful in inducing early sporulation and increased content of glycogen. Phosphate and calcium also affected glycogen accumulation significantly, although, the sporulation was found to be of the same order as in nitrogen free medium. Sulphate initiated early sporulation, the mechanism of which is not known.
The effect of 21 amino acids was studied on glycogen accumulation during sporulation in the blue-green alga Anabaena sp. All the amino acids enhanced the initial level of glycogen on the 4th day. The maximum amount of glycogen, on the 20th day, was noticed from L-methionine, L-tyrosine, glycine, and L-histidine supplemented cultures. Others like L-serine, L-valine, L-asparagine, DL-alanine, L-glutamic acid, L-phenylalanine, L-aspartic acid, L-arginine, and DL-lysine come next in the order. On the other hand, L-cysteine and L-cystine, although upto the the 16th day they exhibited higher values of glycogen, did not show much variation in glycogen content over nitrogen-free medium. Except in these two amino acids, in all others initiation of sporulation occurred on the 4th day, resulting in free spores on the 8th day. But in case of L-serine, L-asparagine, L-isoleucine, and L-phenylalanine free spores were noticed only on the 12th day while in DL-lysine they were seen on the 16th day. L-cysteine and L-cystine supplemented media showed free spores on the 20th day as in nitrogen-free control.
Glycogen accumulation in vegetative cells of Anabaena sp. is demonstrated to be a light-dependent process. No glycogen accumulation is found in dark or in cultures supplemented with 10(-5) M DCMU in light. Large quantities of glycogen accumulate in cells undergoing sporulation and the amount increased with the onset of maturation of spores.
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A case of hemangioendothelioma of the mandible, the third of its kind to involve the jawbone is reported. The radiological appearance of new bone formation is a unique feature in this case. The vascular malignant tumor has a poor prognosis.
A case of melanotic neuroectodermal tumour occurring in a seven-month-old male infant is reported. The aetiopathogenesis, clinical features, treatment and prognosis of the condition are discussed. Tumours of the jaw in infancy are extremely rare, and pigmented tumours still rarer. One such case of pigmented tumour of the maxilla is the subject of this report.