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S Keck

Publications and source records attributed to S Keck.

5 recordsLinked to original sources
PubMed1989

Detection and partial characterization of a developmentally regulated nuclear antigen in neural cells in vitro and in vivo.

We report that a monoclonal antibody directed against phosphorylated neurofilaments (SMI 31) recognizes nuclear antigens present in embryonic but not in adult neural cells. On Western blots, the antibody reacts with four proteins of apparent MW 35, 37, 52/54, and 250 KD which are found exclusively in developing brain tissue. These nuclear antigens are expressed by glial and neuronal cells. Both nuclear staining and immunoreactive proteins decrease with ongoing in vitro differentiation. A computer search for proteins that share the epitope recognized by antibody SMI 31 did not yield any proteins of known nuclear localization that exhibit the same molecular weights and solubility characteristics as the above immunoreactive proteins. We conclude that antibody SMI 31 recognizes hitherto unknown nuclear proteins which, in neural cells, are developmentally regulated.

Animals↗
PubMed1983

Cardiac tamponade: an initial study in the development of a predictive tool.

This exploratory study was conducted over a 12-month period to describe the relationship between selected clinical variables and the occurrence of postoperative cardiac tamponade. Data were collected on 19 variables. A total of 739 patients were included in the study. A postoperative diagnosis of cardiac tamponade was made in nine of these patients who then comprised the tamponade group. A matched non-tamponade control group of 18 patients was identified for comparison. The following were found to be significantly different in a statistical comparison between variable values in the tamponade and non-tamponade groups: (1) serum creatinine level (p = 0.005), (2) chest tube drainage (p = 0.009), (3) sustained pressure plateau (p = .018), and (4) mediastinal widening (p = 0.039). Two additional variables, pulsus paradoxus and elevated BUN, appeared to be of value in the assessment of late tamponade. Discriminant analysis showed a high predictive accuracy of the above combined variables. Such results warrant the further investigation of these variables in other clinical settings. Both a replication and testing of a tamponade score system would be a fertile and necessary area of research for critical care nurses who provide care for post-cardiac surgery patients. These data suggest that equal weight be assigned to each of the four variables. Thus scores would range from 0 to 4; higher scores would represent greater risk (Table III). With further investigation of this area of clinical practice, the identification of patients at higher risk for tamponade could be greatly facilitated.

Aged↗