Biomedical subjects

S Keeney

Publications and source records attributed to S Keeney.

3 recordsLinked to original sources

PubMed1992

Biochemical heterogeneity in xeroderma pigmentosum complementation group E.

Cells from two patients with xeroderma pigmentosum complementation group E (XP-E) have been shown to lack an activity which binds specifically to UV-irradiated DNA (Chu and Chang, 1988). We investigated the occurrence of this binding activity in cell strains from nine additional, unrelated XP-E patients and found that all but one of these strains contained normal levels of the binding protein. Furthermore, the binding activity from these XP-E strains was indistinguishable from that of normal controls in thermal stability, behavior on ion-exchange chromatography, and electrophoretic mobility of protein-DNA complexes, indicating that there were no gross structural alterations in the protein. The association of XP-E with a deficiency in DNA-damage binding protein in cells from 3 of 12 XP-E patients (compared to 0 of 20 non-XP-E controls) is statistically significant (p less than 0.05), but there is no obvious correlation between the biochemical defect and the clinical or cellular characteristics of individual patients. Implications of these findings for the role of the binding protein in XP-E are discussed.

Cells, Cultured↗

PubMed1992

Neonatal transport in Texas.

The critically ill newborn requires specialized care, which is generally provided in neonatal intensive care units (NICUs). We surveyed all identifiable NICUs in the state of Texas to study deficiencies in the current system. Despite the existence of many neonatal transport teams, little or nothing has been done to coordinate similar efforts at different programs or to organize programs into sharing the responsibility of transport among the less served areas of Texas. The lack of adequate reimbursement may be a limiting factor in the organization and regionalization of neonatal transport. We urge further efforts toward coordination and regionalization of transport with an examination of reimbursement policies to allow teams to share equally the responsibility of neonatal transport in Texas.

Catchment Area, Health↗

PubMedDate not supplied

A critical review of permeabilized cell systems for studying mammalian DNA repair.

Permeabilized cell systems have proven valuable for studies of the enzymology of mammalian DNA repair and this review will summarize and contrast the different systems used to this end. Results from permeable cell studies will be reviewed which pertain to 3 questions of DNA repair: the role(s) of ATP, DNA polymerase enzymology, and the isolation of repair factors by in vitro correction of repair-defective cells.

Adenosine Triphosphate↗