Biomedical subjects

S Kemper

Publications and source records attributed to S Kemper.

At least 19 recordsLinked to original sources

PubMed2005

Highly diverse variable number tandem repeat loci in the E. coli O157:H7 and O55:H7 genomes for high-resolution molecular typing.

AIM: Evaluation of the Escherichia coli genome for variable number tandem repeat (VNTR) loci in order to provide a subtyping tool with greater discrimination and more efficient capacity. METHODS AND RESULTS: Twenty-nine putative VNTR loci were identified from the E. coli genomic sequence. Their variability was validated by characterizing the number of repeats at each locus in a set of 56 E. coli O157:H7/HN and O55:H7 isolates. An optimized multiplex assay system was developed to facility high capacity analysis. Locus diversity values ranged from 0.23 to 0.95 while the number of alleles ranged from two to 29. This multiple-locus VNTR analysis (MLVA) data was used to describe genetic relationships among these isolates and was compared with PFGE (pulse field gel electrophoresis) data from a subset of the same strains. Genetic similarity values were highly correlated between the two approaches, through MLVA was capable of discrimination amongst closely related isolates when PFGE similar values were equal to 1.0. CONCLUSIONS: Highly variable VNTR loci exist in the E. coli O157:H7 genome and are excellent estimators of genetic relationships, in particular for closely related isolates. SIGNIFICANCE AND IMPACT OF THE STUDY: Escherichia coli O157:H7 MLVA offers a complimentary analysis to the more traditional PFGE approach. Application of MLVA to an outbreak cluster could generate superior molecular epidemiology and result in a more effective public health response.

Alleles↗

PubMed2004

Factors associated with residual breast cancer after re-excision for close or positive margins.

BACKGROUND: Successful breast conservation surgery (BCS) requires complete tumor excision. Margin status of the initial specimen determines the need for additional surgery. We explored factors associated with residual cancer (RC) upon follow-up surgery in patients with close, positive, or undetermined margins following BCS. METHODS: A retrospective analysis of 276 patients with initial close, positive, or undetermined margins who underwent re-excision (RE) or mastectomy was conducted. All initial excisions were intended as definitive procedures. Chi-square analysis was used to identify factors that may predict RC. RESULTS: Of 276 patients, 87 had close, 168 had positive, and 21 had undetermined margins on initial excision. Of this group, 63% (175/276) had RC upon RE or mastectomy. Of positive-margin patients, 68% had RC, compared with 53% of close-margin and 67% of undetermined-margin patients (P = .006). Tumors >/=2 cm were more often associated with RC than smaller tumors (70.8% vs. 56.5%; P = .07). This association was strongest in positive-margin patients (P = .04). High tumor grade was associated with RC in all groups. RC linearly increased with the number of involved margins (P = .02). Specimen inking with multiple colors was associated with decreased risk of RC (P = .004). CONCLUSIONS: Over half of patients with involved or undetermined margins had RC upon RE or mastectomy. Positive and undetermined margins were more often associated with RC than close margins. Larger tumor size was associated with RC in patients with positive. Increasing tumor grade suggests a greater chance of detecting RC in all groups. Multiple involved margins led to a greater risk of RC.

Adult↗

PubMed2002

Pharmacokinetics of a new transdermal testosterone gel in gonadotrophin-suppressed normal men.

OBJECTIVE: In a phase I single-centre, open, randomized study, the pharmacokinetics of two doses of a transdermal testosterone gel containing 2.5% testosterone were evaluated in 26 healthy male volunteers. DESIGN: To eliminate the influence of endogenous serum testosterone, gonadotrophins and endogenous testosterone secretion were suppressed by a single intramuscular injection of 400 mg norethisterone enanthate. Fourteen men applied 5.0 g and 12 men applied 2.5 g testosterone gel daily for 10 days. Half the men in each group washed the gel off 10 min after it had been applied. RESULTS: In all the men, a marked suppression of LH, FSH, testosterone, dihydrotestosterone (DHT) and oestradiol was observed after norethisterone treatment. Physiological serum concentrations of testosterone were restored during the 10-day treatment period in the group of men applying 5.0 g testosterone gel. Increasing serum concentrations of testosterone from day 1 to day 10 were observed. Oestradiol and DHT concentrations did not exceed normal values. Washing 10 min after gel application did not influence the resorption of testosterone. A dose of 2.5 g testosterone gel was insufficient to achieve physiological serum concentrations of testosterone. CONCLUSION: Testosterone replacement treatment with 5.0 g of this 2.5% testosterone gel is able to achieve constant physiological testosterone concentrations in gonadotrophin-suppressed men. Washing the skin after 10 min does not influence the pharmacokinetic profile and thus significantly reduces the risk of contamination of female partners or infants.

Adult↗

PubMed2001

Selective insulin signaling through A and B insulin receptors regulates transcription of insulin and glucokinase genes in pancreatic beta cells.

Insulin signaling is mediated by a complex network of diverging and converging pathways, with alternative proteins and isoforms at almost every step in the process. We show here that insulin activates the transcription of its own gene and that of the beta cell glucokinase gene (betaGK) by different mechanisms. Whereas insulin gene transcription is promoted by signaling through insulin receptor A type (Ex11-), PI3K class Ia, and p70s6k, insulin stimulates the betaGK gene by signaling via insulin receptor B type (Ex11+), PI3K class II-like activity, and PKB (c-Akt). Our data provide evidence for selectivity in insulin action via the two isoforms of the insulin receptor, the molecular basis being preferential signaling through different PI3K and protein kinases.

Calcium Channel Blockers↗

PubMed2001

Longitudinal change in language production: effects of aging and dementia on grammatical complexity and propositional content.

Mixed modeling was used to examine longitudinal changes in linguistic ability in healthy older adults and older adults with dementia. Language samples, vocabulary scores, and digit span scores were collected annually from healthy older adults and semiannually from older adults with dementia. The language samples were scored for grammatical complexity and propositional content. For the healthy group, age-related declines in grammatical complexity and propositional content were observed. The declines were most rapid in the mid 70s. For the group with dementia, grammatical complexity and propositional content also declined over time, regardless of age. Rates of decline were uniform across individuals. These analyses reveal how both grammatical complexity and propositional content are related to late-life changes in cognition in healthy older adults aswell as those with dementia. Alzheimer's disease accelerates this decline, regardless of age.

Aged↗

PubMed2001

Language decline across the life span: findings from the Nun Study.

The present study examines language samples from the Nun Study. Measures of grammatical complexity and idea density were obtained from autobiographies written over a 60-year span. Participants who had met criteria for dementia were contrasted with those who did not. Grammatical complexity initially averaged 4.78 (on a 0-to-7-point scale) for participants who did not meet criteria for dementia and declined .04 units per year; grammatical complexity for participants who met criteria for dementia initially averaged 3.86 and declined .03 units per year. Idea density averaged 5.35 propositions per 10 words initially for participants who did not meet criteria for dementia and declined an average of .03 units per year, whereas idea density averaged 4.34 propositions per 10 words initially for participants who met criteria for dementia and declined .02 units per year. Adult experiences, in general, did not moderate these declines.

Adult↗

PubMed2001

The structure of verbal abilities in young and older adults.

Four language sample measures as well as measures of vocabulary, verbal fluency, and memory span were obtained from a sample of young adults and a sample of older adults. Factor analysis was used to analyze the structure of the vocabulary, fluency, and span measures for each age group. Then an "extension" analysis was performed by using structural modeling techniques to determine how the language sample measures were related to the other measures. The measure of grammatical complexity was associated with measures of working memory including reading span and digit span. Two measures, sentence length in words and a measure of lexical diversity, were associated with the vocabulary measures. The fourth measure, propositional density, was associated with the fluency measures as a measure of processing efficiency. The structure of verbal abilities in young and older adults is somewhat different, suggesting age differences in processing efficiency.

Adult↗

PubMed2000

Sentence repetition and processing resources in Alzheimer's disease.

Sentence processing in Alzheimer's disease (AD) has been found to be influenced by several grammatical and extragrammatical factors, including phrase structure and verb-argument relations, number of propositions/verbs, and processing resource capacity. This study examines the effects of these variables on sentence production in AD. Normal control and AD subjects were asked to repeat six types of sentences varying along the above dimensions of complexity. Subjects' processing resource capacity was measured using several verbal working memory tests. AD subjects' sentence-repetition performance was impaired compared to the normal control group. Significant effects were observed for branching direction of phrase structure, canonicity of verb-argument relations, and serial position of errors. Sentence-repetition performance significantly correlated with working memory scores. The findings are interpreted within a resource capacity theory of sentence processing.

Aged↗

PubMed1999

Experimentally disentangling what's beneficial about elderspeak from what's not.

Three studies evaluated different varieties of elderspeak using a referential communication task. Experiment 1 compared the effects of syntactic simplifications and semantic elaborations. Experiment 2 contrasted syntactic simplifications and prosodic exaggerations. Experiment 3 contrasted 2 different syntactic simplification strategies and 2 different prosodic exaggerations. Providing semantic elaborations and reducing the use of subordinate and embedded clauses benefit older adults and improve their performance on the referential communication task, whereas reducing sentence length, slowing speaking rate, and using high pitch do not. The use of short sentences, a slow rate of speaking, and high pitch resulted in older adults' reporting more communication problems. These experiments validate a version of elderspeak that benefits older adults without sounding patronizing and insulting.

Adult↗

PubMed1999

Aging and resolution of quantifier scope effects.

Two experiments were conducted to compare young and older adults' processing of complex sentences involving quantifier scope ambiguities. Young adults were hypothesized to use a mix of syntactic processing strategies to interpret sentences such as Every actor used a prop or An actor used every prop. Older adults, particularly those with limited working memories, were hypothesized to rely on a simple pragmatic principle. Participants read the quantifier sentences and judged whether a continuation sentence "made sense." Reading times for the quantifier sentences and decision times and continuation sentence acceptability judgements were analyzed. Whereas young and older adults exhibited similar patterns of reading times for the quantifier sentences, they preferred different continuations for the Every ... a quantifier sentences. As predicted, both young adults and older adults interpreted a quantifier sentence such as An actor used every prop as referring to a single entity resulting in a preference for continuations such as The actor was on the stage. In contrast, young and older adults made different interpretations of a quantifier sentence such as Every actor used a prop; young adults preferred continuations postulating multiple entities such as The props were on the stage whereas older adults, particularly those with working memory limitations, preferred continuations with a single entity such as The prop was on the stage. These results support models of the effects of aging on language processing in which immediate syntactic analysis is not affected by aging or working memory limitations whereas postcomprehension processes are affected by aging and/or working memory limitations.

Adolescent↗

PubMed1997

Grammatical abilities in Parkinson's disease: evidence from written sentences.

This study examined the grammatical content of written sentences elicited from 96 Parkinson's patients, 30 Parkinson's with dementia patients and 167 control subjects. Parkinson's patients without dementia or with mild dementia presented no impairments in sentence length, syntactic complexity or amount of information content. Moderately demented Parkinson's patients showed reduced sentence length and information content but normal syntactic complexity. This pattern of results provides evidence that lexical-semantic content is more susceptible to decline than syntactic structure with the progression of dementia in Parkinson's disease.

Aged↗

PubMed1997

Sentence comprehension in Alzheimer's disease: effects of grammatical complexity, speech rate, and repetition.

Caregivers of patients diagnosed with Alzheimer's disease (AD) are often advised to modify their speech to facilitate the patients' sentence comprehension. Three common recommendations are to (a) speak in simple sentences, (b) speak slowly, and (c) repeat one's utterance, using the same words. These three speech modifications were experimentally manipulated in order to investigate their individual and combined effects on sentence comprehension in AD. Fifteen patients with mild to moderate AD and 20 healthy older persons were tested on a sentence comprehension task with sentences varying in terms of (a) degree of grammatical complexity, (b) rate of presentation (normal vs. slow), and (c) form of repetition (verbatim vs. paraphrase). The results indicated a significant decline in sentence comprehension for the AD group. Sentence comprehension improved, however, after the sentence was repeated in either verbatim or parapharsed form. However, the patients' comprehension did not improve for sentences presented at the slow speech rate. This pattern of results is explained via-à-vis the patients' working memory loss. The findings challenge the appropriateness of several clinical recommendations.

Aged↗

PubMed1997

Younger and older adults' on-line processing of syntactically ambiguous sentences.

Off-line studies of younger and older adults' processing of syntactically complex sentences have shown that there is a consistent negative relationship between task performance and working memory for older adults. However, it is not evident from these studies whether working memory affects the immediate syntactic analysis of a sentence, off-line processes, or both. In the current study an online reading paradigm was used to examine the working memory capacity-constrained sentence processing model from M.C. MacDonald, M.A. Just, and P.A. Carpenter (1992). Working memory span, type of syntactic ambiguity (ambiguous vs. unambiguous), and type of syntactic ambiguity resolution (main verb vs. relative clause) interacted to influence younger and older adults' on-line reading times and off-line sentence comprehension.

Adolescent↗

PubMed1997

Metalinguistic judgments in normal aging and Alzheimer's disease.

This study compared sentence acceptability judgments from young and healthy older adults and older adults with dementia due to probable Alzheimer's disease. Two types of sentences were contrasted: one type involved contrasts among verb alternations in which semantic distinctions between verbs regulate the acceptability of phrase structure variations; the second type involved contrasts among directional transformations in which constraints on the movement of noun phrases determine the linear order of main and embedded clauses. The primary findings were that metalinguistic judgments by healthy older adults as well as those with probable Alzheimer's reflected processing demands on working memory. In addition, metalinguistic judgments by adults with probable Alzheimer's reflected the breakdown of semantic information about verbs.

Adolescent↗

PubMed1997

Standardized assessment of psychological well-being and quality-of-life in patients with implanted defibrillators.

The ICD has become a standard treatment for patients with malignant arrhythmias. Despite its benefits it may cause additional discomfort to the patients. Thus, quality-of-life needs to be assessed in these patients. Previous studies have used only small samples or unstandardized measures of quality-of-life that do not allow comparisons with other patient groups. The present study used standardized questionnaires for a cross-sectional assessment of psychological well-being and quality-of-life in ICD patients and to compare them to a similar group of coronary artery disease (CAD) patients without ICD. Overall, quality-of-life did not differ between both groups, ICD patients being less anxious than the CAD group. With increasing numbers of ICD shocks, however, the percentage of psychologically distressed ICD patients rose from 10% to > 50%. Psychologically distressed patients had significantly worse scores on most of the quality-of-life subscales, showed less treatment satisfaction, and more negative attitudes. It is concluded that ICD patients have an acceptable mean quality-of-life and low mean anxiety. However, a relevant subgroup of about 15%, especially patients with frequent shocks, experience psychological distress and reduced quality-of-life and should receive special care.

Adaptation, Psychological↗

PubMed1994

Speech accommodations to dementia.

This study investigated whether spouses would adopt a specialized speech register when communicating with adults with probable Alzheimer's disease. A picture description task was used so that the effectiveness of such speech accommodations could be assessed. The AD subjects did not vary the syntactic complexity, semantic complexity, or content of their descriptions when they were describing individual pictures versus directing their spouse to choose one of four pictures in a barrier task. The spouses' picture descriptions were more complex syntactically and semantically than the AD subjects' and included more highly salient elements. The spouses also varied the complexity and content of their descriptions, reducing syntactic and semantic complexity and increasing references to highly salient picture elements during the barrier task. These accommodations appeared to facilitate the AD subjects' performance on the picture description task.

Aged↗

PubMed1993

On the preservation of syntax in Alzheimer's disease. Evidence from written sentences.

We examined the syntactic complexity of single written sentences elicited from 368 adults undergoing examination for possible Alzheimer's disease. The clause length and composition of the sentences varied with the severity of dementia. The nondemented adults' sentences contained more propositions, main and secondary verbs, and conjunctions than those produced by the mildly and moderately demented adults. Sentence length in clauses, propositional content, and the use of conjunctions and main and secondary verbs discriminated among stages of the severity of the disease. The present results suggest that, while dementia severity affects written linguistic output, such output is, nonetheless, grammatic and coherent.

Aged↗

PubMed1993

Recall and articulation of English and Chinese words by Chinese-English bilinguals.

Three groups of subjects were tested to investigate the effect of language on the relationship between recall span and articulation rate. Native English-speaking monolinguals and native Chinese-speaking monolinguals recalled only English or Chinese words, respectively. Chinese-English bilinguals recalled both English and Chinese words. Articulation rates for English and Chinese monolinguals and Chinese-English bilinguals in each language were also obtained. When recall span was regressed on articulation rate, the slopes for Chinese and English words were significantly different for the Chinese-English bilinguals. This difference was not due to language proficiency but to phonological differences between English and Chinese.

Adult↗