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Biomedical subjects

S Kneist

Publications and source records attributed to S Kneist.

At least 19 recordsLinked to original sources

[The distribution of mutans streptococci and lactobacilli depending on the caries prevalence in 11- to 12-year-old children].

20 Korean children, aged 11 to 12 years, participated in this study with the aim to compare the microbial composition of plaque samples in order to estimate, which genera (lactobacilli, streptococci) or species are associated with coronal caries. Significant differences in the microbial composition of plaque samples between children with healthy and carious dentitions were found. S. mutans (c, e, f) was dominating under Mutans-streptococci.

Child

[Clinical controlled study on the treatment of deep carious lesions in deciduous molars].

Compared with a step-by-step procedure, the one-step excavation of deep carious lesions in primary molars proved to be the treatment of choice in a clinically, microbiologically and histologically controlled trial. The stepwise method failed to produce any significant reduction in pulp exposure. Sensitivity assessment and percussion findings did not result in a reliable and unambiguous evaluation of treatment success. Histology showed inflammation-free pulps in 67% of the deciduous molars irrespective of the treatment method used. Microbiological examination revealed slightly softened dentin to be significantly more infected than clinically acceptable, hard dentin; although only 59.3% of the cavity floors were free of microorganisms. Chronic pulp inflammations were correlated with the presence of streptococci and lactobacilli, whereas actinomycetes were associated with inflammation-free pulps.

Case-Control Studies

[The establishment efficiency of S. sobrinus OMZ 176 in a model caries experiment on the Osborne-Mendel rat].

S. sobrinus OMZ 176 was inoculated in young OM rats during tooth eruption in two experiments and the microbial composition of dental plaque as well as the caries decay was estimated after 25 days on cariogenic sucrose rich diet. Germs were reproducibly established in the oral cavity by such way. The resulting caries decay correlated with the streptococci in the dental plaque. Due to this fact it seems to be necessary to identify not only the inoculated microorganisms at the end of each animal experiment but also to estimate the qualitative composition of the whole dental flora which may have an essential cariogenic effect.

Animals

[The microbial colonization of the dentin close to the pulp in the permanent teeth following deep caries therapy].

In a clinical microbiological study the microflora of cavity floors in 41 permanent teeth was investigated after several month of caries excavation and with or without Calxyl application on hard dentine prior sealing with zincphosphatecementum and amalgam. The cavity floors were only lowly infected. Actinomycetes or acidogenic and aciduric streptococci and lactobacilli dominated in the CFU. After application of Calxyl actinomycetes (A. israelii) were found 3-fold more frequently in the remaining population than streptococci (S. mutans) and lactobacilli. Without application of Calxyl their ratio was 1:1. S. mutans seems to be important for the carious progression in dentin.

Actinomyces

[Root caries--scanning electron microscopic observations].

Sound and carious root surfaces of 24 extracted human teeth with extensive periodontal attachment loss were examined by SEM. The microflora covering the radicular surfaces was a complex flora consisting of filamentous and fusiform bacteria, short and long rods. Cocci and coccoid bacteria were observed on root surfaces. Bacterial invasion in the exposed peripheral root dentin was delayed by sclerotic dentin.

Adult

[Oral Streptococci - a scheme for the identification of Streptococcus mutans].

A reliable simple scheme for the rapid identification of certain species of oral streptococci has been developed and compared with biochemical and physiological results of 45 well known clinical isolates and stock strains. Moreover, a method for the determination of H2O2 production was tested. With selected reactions as for instance acid formation in mannitol and raffinose broth, hydrolysis of arginine and esculin, the production of peroxidase, and the resistance of bacitracin the species S. rattus, S. sobrinus, S. mutans, S. cricetus, S. ferus, S. milleri, S. mitis, S. sanguis, S. mitior, and S. salivarius were differentiated.

Animals

[Root caries--a rat model].

A increasing alveolar bone loss and typical root surface caries were reproduced after defined co-inoculation of germfree Wistar rats with S. mutans and A. viscosus. The gnotobiotic rats were re-conventionalized and performed a gingivectomy on the right upper jaw at the beginning of the 12 week experiment.

Actinomyces

[Cotton roll or rubber dam in endodontic treatment?].

In a clinical-microbiological study of the bacterial flora on deep areas of carious dentine in deciduous and permanent human teeth the aseptic rubber dam technique was given preference to remove successive samples of dentine from the cavity floors. The application of the rubber dam made it possible to establish and maintain a surgically clean field of operation and were an effective safeguard against contamination through seepage of saliva.

Dentistry, Operative

[Microbial colonization of carious progression stages in the dentin of human teeth--a controlled therapeutic study].

In a controlled clinical trial the microflora of the cavity floor of 70 primary lower second molars with deep carious lesions were determined after caries excavation. The teeth were extracted and pulpal status was evaluated after 16 months of microbial control to determine the etiopathogenic role of germs for carious progression in dentine. 67% of the primary molars were free from pulpal inflammations. Soft carious dentine were significantly higher infected than the clinically acceptable hard dentine. Only in 40% of the cavity floors the microorganisms were eliminated. In the infected teeth basophilic microorganisms were found in causality to pulps without inflammations; acidogenic streptococci and lactobacilli were involved in pulpal inflammations. Results indicate that the latter genera of microorganisms are of etiological significance for carious progression in dentine.

Actinomycetales