[Androgen receptors in the rat submandibular salivary gland. Release of dihydrotestosterone (DHT) binding protein from androgen binding protein in the cytosol].
Explore the source record for details and available documents.
Animals↗
Biomedical subjects
S Koshika
Publications and source records attributed to S Koshika.
[Changes in testosterone and dihydrotestosterone in the rat submandibular gland during development].
The present investigation deals with the changing profile of testosterone (T) and 5 alpha-dihydrotestosterone (DHT) concentrations in the rat submandibular gland (SMG) with age. T and DHT concentrations in SMG from male and female Wistar Rats 0 to 10 weeks of age were measured by a specific radioimmunoassay. T and DHT in male and female SMG showed lower values until 3 weeks (4.5 +/- 1.5 ng/g). However, at 4 weeks of age, T levels greatly increased, revealing 15.1 +/- 6.3 ng/g for males and 18.4 +/- 6.6 ng/g for females. And thereafter from 5 to 10 weeks of age, T concentrations in SMG were decreased to 3--5 ng/g. On the other hand, from 5 to 10 weeks of age, DHT concentrations in both males and females had a tendency to be accumulated, indicating approximately 1.5--3 times higher than T. It can be reasonably concluded that DHT began to be accumulated in SMG at the period of 4 to 5 weeks of age. Furthermore, it is suggested that the rat SMG demands androgen from approximately 4 weeks of age for growth and function.
Aging↗
Stimulation of motility of human renal cell carcinoma by SPARC/Osteonectin/BM-40 associated with type IV collagen.
SPARC is known to be important in development and tissue remodelling. Here, we examined the effects of SPARC (secreted protein, acidic and rich in cysteine; osteonectin) derived from a rat osteosarcoma cell line on migration of renal cell carcinoma (RCC) by a Boyden chamber assay. YCR RCC cells migrated through type IV collagen-coated filters without stimuli (basal level). SPARC in the lower compartment stimulated chemotactic activity to 120% of the basal level, whereas premixing of YCR with purified SPARC before inoculation reduced their migration to 72% of the basal level. Furthermore, SPARC mixed with type IV collagen more efficiently stimulated their migration in a concentration-dependent manner (up to 170% of the basal level). This suggests that SPARC bound to type IV collagen plays a role in tumor invasion.
Animals↗