PubMed Health⌕ Search

Biomedical subjects

S Kuştimur

Publications and source records attributed to S Kuştimur.

13 recordsLinked to original sources
PubMed1997

Effect of nordihydroguaretic acid and fluconazole on the LTC4/PGE2 ratio in the kidney of mice damaged by Candida albicans.

The kidney is a major target organ in generalized candidiasis. When mice were infected with C.albicans, prostaglandin E2 (PGE2)-like activity was found to be significantly decreased while leukotriene C4 (LTC4)-like activity increased in the kidneys within 10 days. The aim of this study is to investigate the effect of nordihydroguaretic (NDGA) and fluconazole on the LTC4/PGE2 ratio in the mice kidneys infected by proteinase (+) C. albicans. The LTC4/PGE2 ratio was found to be significantly decreased both in NDGA and fluconazole-pretreated groups. These results indicate that pretreatment with the lipoxygenase inhibitor NDGA and the antifungal drug fluconazole, protect the kidney against C. albicans infection. These results also indicate a possible role of arachidonic acid metabolites (increase LTC4 and decrease PGE2) in kidney damage due to C. albicans infection.

Animals↗
PubMed1995

Sample adequacy in detecting Chlamydia trachomatis.

OBJECTIVE: Chlamydia trachomatis is an important etiological agent in female genital infection and may result in infertility. In recent years rapid diagnostic methods have become widely used as alternatives to cell culture. Our objective was to evaluate the technique of direct fluorescence assay (DFA) in estimating the presence of C. trachomatis. METHODS: Specimens taken from 40 infertile and 20 fertile women were examined by DFA for the presence of C. trachomatis. RESULTS: Six of forty (15%) infertile women were found to be positive whereas no positive specimens were detected in the control group. When the specimens were grouped into those which were adequate or inadequate, 19 and five specimens, respectively, were adequate in the infertile and healthy groups. If only adequate specimens are included in estimating the presence of Chlamydia, the percentage is 31.6%. CONCLUSIONS: In order to use DFA as a more reliable and rapid diagnostic test of C. trachomatis in female genital infection, false-negativity must be eliminated. Specimens must be collected adequately and concentrated in order to achieve optimal diagnostic success.

Cervix Uteri↗
PubMed1992

The role of eicosanoids in the kidney damage induced by Candida albicans.

A major target organ in generalized candidiasis is the kidney. The purpose of this study was to investigate the role of eicosanoids in the kidney infected by proteinase-positive and proteinase-negative Candida albicans. Prostaglandin (PG) E2-like activity was found to be significantly decreased while leukotriene (LT) C4-like activity increased within 10 days in the kidneys of mice infected with C. albicans. These results indicate that arachidonic acid metabolism is shifted to the lipoxygenase pathway and lipid peroxides, produced via this enzyme system may play an important role in the kidney damage induced by C. albicans.

Animals↗
PubMed1992

[Antibody levels against coxsackie B viruses in patients with type 1 diabetes mellitus].

In order to detect if the presence of interrelationship between Coxsackie B Viruses (CBV) and etiology of type 1 diabetes mellitus, antibodies to CBV by microneutralization test serotypes 1, 2, 3, 4, 5 and 6 were investigated in 37 sera obtained from patients with type 1 diabetes mellitus and 100 sera from healthy individuals as control group. No significant difference could found that neutralizing antibody levels to CBV serotypes between in sera obtained from patient with type 1 diabetes mellitus and sera obtained from control group. As a result, interrelationship was not detected between CBV infection and having type 1 diabetes mellitus.

Adolescent↗
PubMed1989

[In vitro susceptibility of dermatophyte strains to imidazole derivatives].

Recently, imidazole derivatives are being successfully used for the dermatomycoses. These drugs have the strongest antimycotic activity. 44 dermatophyte strains were isolated from our out-patient with dermatomycosis. Imidazole derivatives such as isoconazole, oxiconazole, bifonazole and tioconazole were studied their efficacy on these fungal agents in vitro. 4 imidazole derivatives were effective on all of the dermatophyte strains in less than or equal to 5 micrograms/ml concentration. Whereas, there were differences in Minimal Inhibition Concentration (MIC) and MIC50 values.

Antifungal Agents↗
PubMed1985

Cerebral cladosporiosis.

A 49-year-old woman was admitted to Hacettepe Medical Faculty Hospital with the complaints of headache, nausea, vomiting, lethargy, and weakness on her right side. She revealed a history of pulmonary Cryptococcus infection 5 years before and she had been treated with amphotericin B. After clinical and laboratory investigation she was thought to have an intracranial mass, but her deteriorating situation did not allow any surgical intervention; she died within 7 days. On necropsy, hard, gray-white nodular pulmonary lesions, ranging 0.1-4 cm in diameter, basal meningitis, infarcts, and a nodular lesion 1.5 cm in diameter similar to those of the lung were present in the white matter of the right hemisphere of the brain. Microscopic examination revealed granulomatous inflammation caused by Cladosporium, which had brown pigment and septate hyphae.

Brain↗
PubMed1985

[Selective IgA deficiency in patients with systemic lupus erythematosus and rheumatoid arthritis].

In this study, antinuclear antibody (ANA), rheumatoid factor (RF) studied and IgA was determined with single radial immunodiffusion (RID) technic in the sera of 96 patients with systemic lupus erythematosus, 25 with rheumatoid arthritis and 100 normal individuals. In normal control group, the average level of IgA was found to be 212 mg/dl. In one patient (1: 100), IgA deficiency was detected with a level of 2.1 mg/dl, the average level of IgA in 96 patients with SLE was 320 +/- 16.25 mg/dl. In 3 patients of this group IgA deficiency was observed. According to this finding, the prevalence of IgA deficiency in the group of SLE ise 1: 32. This prevalence is higher than that of the normal population. The average level of IgA in 25 patients with RA 302.66 +/- 36.57 mg/dl. In this group we observed IgA deficiency only in one patient which means a prevalence of 1: 25, that is higher than the normal group. Reports indicate that the incidence of IgA deficiency in general populations may vary from 1: 400 up to 1: 3080. But in patients with SLE and RA, this may be encountered more frequently as we observed in our cases.

Adolescent↗
PubMed1984

[Salmonella tester strain TA104 for the detection of mutagenic and carcinogenic chemicals in our environment].

We used the Salmonella mutagenicity test for detecting chemical carcinogens as mutagens in the Salmonella typhimurium tester strain TA104 . The mutagenicity of several compounds was assessed by induction of histidine revertants in the TA104 . In each experiment we routinely included positive mutagenesis controls using three different concentrations of known mutagens. The mutagenic chemicals such as sodium azide, hydrogen peroxide and hydroxylamine were found to be mutagenic to TA104 at very low concentration (10(-4) mg/ml). Their mutagenic activity decreased while their concentrations were increased. The effect of acridine orange, 2, 4, 6-trinitrobenzene sulphonic acid, 2- phenylnaphthalene and 20- methylcholanthrene were also found to be mutagenic to TA104 at the concentration of 10(-2) mg/ml. The mutagenicity of other materials such as hair dyes, meat- broth preparations+ and cigarette smoke condensates were also tested, and all of them were found to be mutagenic to TA104 . The highest mutagenic activities were observed at the concentration of 10 mg/ml for two different hair dyes and of 1 mg/ml for cigarette smoke condensates.

Carcinogens, Environmental↗
PubMed1977

[Fungal flora of Ankara air in the morning and evening (author's transl)].

The fungal flora in different areas parts of Ankara in morning and evening atmosfere are discussed. It was seen that Penicillium, and Mycelia sterilia were found more in evenings, when Cladosporium and Alternaria were found in higher concentrations in mornings. Aspergillus was found in evening air either in less or at least in same concentrations. The rate of Monilia in evenings and mornings showed variable concentration according to areas.

Air Microbiology↗
PubMed1977

[Indoor and outdoor fungal flora of Ankara].

The variation of atmospheic fungal flora of Ankara were studied. Beside this the fungal floras of indoor and outdoor air were compared. In outdoor air Penicillium was detected most, followed by Cladosporium, Alternaria, Helminthosporium, Aspergillus, Rhizopus and Mucor. In the indoor air the same fungi were detected. However, Penicillium and Aspergillus were found more compared with outdoor findings. Since in many parts of Ankara (Yenişehir, Cebeci, Küçükesat, Bahçelievler, Aydinlikevler), different fungi are found in indoor and outdoor air and many of them are the etiologic agents of allegic diseases, doctors have to make fungal cultures from the indoor and outdoor air of living and working buildings in order to find the source of allergic diseases.

Air Microbiology↗
PubMedDate not supplied

Fluorometric determination of acid proteinase activity in vulvovaginal candidosis.

Vulvovaginal candidosis is one of the most frequent disorders in obstetrics and gynaecology. Candida albicans is commonly considered to be the true vaginopathic agent. The secreted acid proteinase might be especially relevant in the pathogenesis of vulvovaginal candidosis. A fluorometric determination of acid proteinase activity of clinical C. albicans isolates was carried out during the present work using fluorescamine. L-Leucyl-L-alanine was included as an internal standard and the results were expressed as nmoles of leucylalanine equivalents h-1 per 2 x 10(4) cells. The 13 isolates were taken from non-diabetic, non-pregnant women aged 22-35 years with vulvovaginal candidosis. Candida albicans ATCC 44858 was used as a control. The proteinase activity in culture supernatants was detectable starting from the mid- to late- exponential phase of growth, peaked between 30 and 46 h, and then declined. The control had an activity of 2.72 nmol h-1 per 2 x 10(4) cells, whereas eight of the samples had a lower activity (1.05 nmol h-1 per 2 x 10(4) cells on average) and five of the samples had a higher activity (6.53 nmol h-1 per 2 x 10(4) cells on average). The fluorometric determination of acid proteinase activity was found to be more reproducible and sensitive than the previously used spectrophotometric determinations.

Adult↗
PubMedDate not supplied

[Fungus-induced arthritis caused by Scedosporium apiospermum (Pseudallescheria Boydii)].

Scedosporium (monosporium) apiospermum is the most common causative agent of maduro mycosis. Recent research reports claim that this fungus can also induce arthritis. We report on the disease in a woman from a rural area who was admitted to our clinic because of arthritis symptoms. We were able to detect Scedosporium apiospermum, i.e., it was probably a fungus-induced arthritis which had developed after an intraarticular steroid injection.

Agricultural Workers' Diseases↗