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Biomedical subjects

S L Branton

Publications and source records attributed to S L Branton.

At least 19 recordsLinked to original sources

Effects of S6-strain Mycoplasma gallisepticum inoculation at ten, twenty-two, or forty-five weeks of age on the blood characteristics of commercial egg-laying hens.

In 2 consecutive trials of the current study, the effect of the age of application of S6-strain Mycoplasma gallisepticum (S6MG) inoculation on the blood characteristics of commercial layers housed and maintained under controlled conditions was determined. The ages of inoculation compared were those before lay at 10 wk of age, during onset of lay at 22 wk of age, and during postpeak lay at 45 wk of age. In each trial, hematocrit, plasma protein, and serum cholesterol, triglycerides, and Ca were determined at 20, 24, 32, 43, 47, and 58 wk of age. The data from both trials were pooled then analyzed together, whereas, data from wk 20 (effect of 10-wk S6MG inoculation); data from wk 24, 32, and 43 (effects of 10- and 22-wk S6MG inoculations); and data from wk 47 and 58 (effects of 10-, 22-, and 45-wk S6MG inoculations) were analyzed separately. At wk 20, hematocrit was higher in birds inoculated with S6MG at 10 wk compared with sham-inoculated birds, and across wk 24, 32, and 43, serum Ca was higher in birds inoculated with S6MG at 10 or 22 wk compared with those that were sham-inoculated. Serum Ca level across wk 47 and 58 was higher in birds inoculated with S6MG at 10 wk compared with sham-inoculated controls and birds inoculated with S6MG at 22 wk, with 45-wk S6MG-inoculated birds being intermediate. The response of serum cholesterol level at 47 wk to S6MG inoculation at either 10, 22, or 45 wk compared with controls was nearly opposite to that of the response observed at 58 wk. However, serum triglycerides were depressed only at wk 47 due to the 45-wk S6MG inoculation compared with all other treatments. Variable post-peak alterations in serum Ca and lipids occur in response to the timing of S6MG inoculation in layers housed under controlled conditions.

Aging↗

Stocking density effects on male broilers grown to 1.8 kilograms of body weight.

This study examined the effects of stocking density on live performance, physiological stress level indicators, and processing yields of male broilers grown to 1.8 kg. A total of 3,120 Ross x Ross 708 male chicks was placed into 32 floor pens (5.57 m2/pen). Stocking density treatments were 25, (75 birds/pen), 30 (90 birds/ pen), 35 (105 birds/pen), and 40 (120 birds/pen) kg of BW/m2. The BW gain, feed consumption, and feed conversion were adversely affected with increasing stocking densities by 35 d. Physiological stress indicators (plasma corticosterone, glucose, cholesterol, total nitrites, and heterophil:lymphocyte) were not affected. Litter moisture was higher as stocking density increased, which led to higher footpad lesion scores. In parallel to growth responses, carcass weight was depressed by increasing stocking density, but carcass yield, absolute and relative amounts of abdominal fat, and carcass skin defects were not affected. Increasing stocking density decreased breast fillet weight and its relative yield and breast tender weight, but not breast tender yield. As calculated stocking density increased 5 kg of BW/m2 beyond 25 kg of BW/ m2, final BW and breast fillet weight decreased by 41 and 12 g, respectively. We conclude that increasing stocking density beyond 30 kg of BW/m2 adversely affects growth responses and meat yield of broilers grown to 1.8 kg but does not alter physiological stress indicators.

Animal Nutritional Physiological Phenomena↗

Comparison of Gompertz and neural network models of broiler growth.

Neural networks offer an alternative to regression analysis for biological growth modeling. Very little research has been conducted to model animal growth using artificial neural networks. Twenty-five male chicks (Ross x Ross 308) were raised in an environmental chamber. Body weights were determined daily and feed and water were provided ad libitum. The birds were fed a starter diet (23% CP and 3,200 kcal of ME/kg) from 0 to 21 d, and a grower diet (20% CP and 3,200 kcal of ME/ kg) from 22 to 70 d. Dead and female birds were not included in the study. Average BW of 18 birds were used as the data points for the growth curve to be modeled. Training data consisted of alternate-day weights starting with the first day. Validation data consisted of BW at all other age periods. Comparison was made between the modeling by the Gompertz nonlinear regression equation and neural network modeling. Neural network models were developed with the Neuroshell Predictor. Accuracy of the models was determined by mean square error (MSE), mean absolute deviation (MAD), mean absolute percentage error (MAPE), and bias. The Gompertz equation was fit for the data. Forecasting error measurements were based on the difference between the model and the observed values. For the training data, the lowest MSE, MAD, MAPE, and bias were noted for the neural-developed neural network. For the validation data, the lowest MSE and MAD were noted with the genetic algorithm-developed neural network. Lowest bias was for the neural-developed network. As measured by bias, the Gompertz equation underestimated the values whereas the neural- and genetic-developed neural networks produced little or no overestimation of the observed BW responses. Past studies have attempted to interpret the biological significance of the estimates of the parameters of an equation. However, it may be more practical to ignore the relevance of parameter estimates and focus on the ability to predict responses.

Animals↗

Stocking density and physiological adaptive responses of broilers.

Three trials were conducted to assess the effects of stocking density on physiological adaptive responses of broilers. Male broilers were reared in floor pens under conditions similar to those used commercially in the United States. Accepted indicators of adaptation to a stressor were measured on d 49 including plasma concentrations of corticosterone, glucose, cholesterol, and total nitrites as an indicator of nitric oxide, as well as heterophil:lymphocyte ratio. In trial 1, calculated stocking densities were 20, 25, 30, 35, 40, 45, 50, and 55 kg of BW/ m2 and in trials 2 and 3, stocking densities were 30, 35, 40, and 45 kg of BW/m2. Stocking densities were calculated based on a final BW of 3.3 kg. Linear trend analyses were used to assess the role of stocking density on each of the physiological parameters. Results indicate that stocking density did not cause physiological adaptive changes indicative of stress.

Adaptation, Physiological↗

Effects of S6-strain Mycoplasma gallisepticum inoculation at 10, 22, or 45 weeks of age on the digestive and reproductive organ characteristics of commercial egg-laying hens.

Experimental inoculation of commercial laying hens with the S6-strain of Mycoplasma gallisepticum (S6MG) at 20 wk of age, while being maintained under ideal conditions, has previously been shown to affect the lengths and weights of various portions of the reproductive tract. Two trials were conducted in the current study to compare the effects of S6MG inoculation prior to lay at 10 wk of age, during onset of lay at 22 wk of age, and during lay at 45 wk of age on the digestive and reproductive organs of commercial layers similarly housed and maintained under ideal conditions. In each trial, liver weight, liver moisture and lipid concentration, incidence of fatty liver hemorrhagic syndrome, ovary weight, ovarian mature follicle numbers, weights and lengths of the oviduct and oviductal regions, and weights and lengths of the small intestine and small intestinal regions were examined at 60 wk of hen age. At 60 wk, liver lipid concentration was depressed, and isthmus weight, as a percentage of total oviduct weight, was increased in birds that had been inoculated with S6MG at 45 wk. Alterations in liver lipid content and weight of the isthmal portion of the oviduct may occur in response to S6MG inoculation during the later stages of production in layers housed under ideal conditions.

Age Factors↗

Stress and acid-base balance in chickens.

Two trials were conducted to study the effects of continuous infusion of adrenocorticotropic hormone (ACTH) on acid-base balance in broiler chickens. Osmotic pumps delivered 8 IU of ACTH in saline/kg of BW/d for 7 d or the same saline volume as used in ACTH at 1 microL/h for 7 d. Blood samples were taken on d 0 (baseline values) and on d 4, 7, and 14 after onset of the infusions. The ACTH treatment increased the hematocrit, partial pressure of CO2, anion gap, corticosterone, mean corpuscular hemoglobin concentration, the blood concentrations of hemoglobin and HCO3-, and reduced the partial pressure of O2, plasma concentrations of Na+, K+, and Cl-. Blood pH values and plasma concentrations of Ca2+ were unaffected by ACTH treatment. The ACTH infusion also resulted in a significant increase in plasma glucose, cholesterone, high-density lipoprotein, and triglyceride. There were no differences in any of the blood constituents measured from control groups. Results indicate that infusion of ACTH resulted in changes in plasma acid-base status along with changes in other blood metabolic variables. However, the ACTH treatment did not prevent homeostatic regulation of acid-base balance, as indicated by constant blood pH. There was, however, an increased need for O2 to support gluconeogenic energy production; the birds responded by increased erythropoiesis. This adaptive response provided greater numbers of erythrocytes and thus a higher amount of circulating hemoglobin to deliver O2 for metabolism.

Acid-Base Equilibrium↗

Effects of S6-strain Mycoplasma gallisepticum inoculation at ten, twenty-two, or forty-five weeks of age on the egg yolk composition of commercial egg-laying hens.

Commercial laying hens maintained under controlled conditions were experimentally inoculated with the S6 strain of Mycoplasma gallisepticum (S6MG) at 45 wk of age. This resulted in depressed liver lipid concentration, and inoculations at 20 and 45 wk affected the size of various portions of the reproductive tract. In 2 consecutive trials of the current study, the effect of age of application of S6MG inoculation on the egg yolk characteristics of commercial layers similarly housed and maintained under controlled conditions was determined. The ages of inoculation compared were prior to lay at 10 wk of age, during onset of lay at 22 wk of age, and during postpeak lay at 45 wk of age. In each trial, yolk moisture and total lipid content were determined at 24, 32, 43, 47, and 58 wk of age. Yolk cholesterol concentration and yolk fatty acid profiles at wk 47 and 58 were also examined. Data from wk 24, 32, and 43 (effects of S6MG inoculations at 10 and 22 wk) and data from wk 47 and 58 (effects of S6MG inoculations at 10, 22, and 45 wk) were analyzed separately. The data of both trials were pooled then analyzed together. Across wk 47 and 58, percentage yolk lipid was significantly lower in eggs laid by birds inoculated at 10 wk compared with those inoculated at 45 wk. Sham-inoculated control and 22-wk inoculated groups had intermediate percentage yolk lipids. Compared with sham-control and 10-wk S6MG inoculation groups across wk 47 and 58, yolk myristic, oleic, and linolenic acid concentrations were reduced, whereas yolk stearic and arachidonic acid levels were increased by either 22- or 45-wk S6MG inoculations. In comparison with all other treatment groups at wk 47, yolk linoleic acid concentration was reduced by S6MG inoculation at 45 wk. Variable postpeak alterations in yolk total lipid and fatty acid content occur in response to the timing of S6MG inoculation in layers housed under controlled conditions.

Aging↗

Ocular responses to ammonia in broiler chickens.

In two trials, 60 male commercial broilers were placed in each of eight environmentally controlled chambers receiving 0, 25, 50, or 75 ppm aerial ammonia from 1 to 28 days. Birds exposed to 25 ppm (lower concentration) ammonia gas developed ocular abnormalities but at a slower rate when compared with birds exposed to 50 and 75 ppm (higher concentrations). Birds exposed to higher concentrations also developed more severe lesions. With little atmospheric ammonia present after 28 days of the grow-out stage, the corneas indicated signs of healing. Lymphocytes and heterophils were seen in the iris at 49 days in ammonia-exposed birds even when ammonia exposure was terminated at 28 days. The lower ammonia concentrations resulted in abnormalities that were slight when compared with those seen at the higher ammonia concentrations. As measured by the incidence of inflammatory infiltrates in the trachea, lung, and air sacs, respiratory tract tissues did not appear to be affected by any tested level of aerial ammonia. The findings in this investigation represent the first report indicating that ammonia-induced uveitis in chickens clears rapidly after exposure to ammonia ceases.

Ammonia↗

Breeder hen dietary L-carnitine affects progeny carcase traits.

(1) Ross 308 broiler breeder hens were given diets containing 0 or 25 mg L-carnitine/kg from 21 weeks of age. (2) Hens were inseminated with semen from Ross broiler breeder males and subsequent growth performance and carcase traits, of progeny obtained from hatches at 30, 35 and 37 weeks of age, were evaluated. (3) Progeny were hatched in a common facility and separated by gender. Experimental treatments employed for the 30-, 35- and 37-week hatches, respectively, were: hen diet and progeny gender (16 replications with two subplots); hen diet, progeny diet (0 and 50 mg L-carnitine/kg of diet) and progeny gender (16 replications with 4 subplots); and hen diet and progeny diet (high and low density; 16 replications with two subplots). (4) Females had lower growth rate and less breast meat, but greater proportions of carcase fat and breast meat than males. Growth performance measurements of progeny were not affected by hen L-carnitine, but hen L-carnitine decreased abdominal fat in progeny. Increasing diet density in the chick diets increased growth and carcase weights. Hen and progeny dietary L-carnitine interacted to increase male mortality. However, dietary hen L-carnitine decreased carcase fat and increased breast meat in progeny fed on high nutrient density diets. (5) In conclusion, L-carnitine in the diet of hens affected carcase traits of their progeny.

Animal Feed↗

Effects of S6-strain Mycoplasma gallisepticum inoculation at ten, twenty-two, or forty-five weeks of age on the performance characteristics of commercial egg laying hens.

Experimental inoculation of commercial laying hens, maintained under controlled conditions, with the S6-strain of Mycoplasma gallisepticum (S6MG) at 10 wk of age has previously been shown to affect the lengths and weights of various portions of the reproductive tract without affecting subsequent performance. Two trials were conducted to compare the effects of S6MG inoculation at 10 wk of age (prior to lay), 22 wk of age (onset of lay), and 45 wk of age (during lay) on performance characteristics in commercial layers housed and maintained under controlled conditions, as in previous studies. In each trial, BW, mortality, egg production, egg weight, eggshell weight per unit of surface area, percentage eggshell weight, percentage albumen weight, percentage yolk weight, and yolk weight per albumen weight ratio were examined at various ages throughout an entire laying cycle. Across wk 47 and 58 (age periods after the last 45 wk inoculation), eggshell weight per unit of surface area and percentage eggshell weight were significantly reduced in birds that had received an S6MG inoculation at 45 wk of age when compared with birds that had not received an S6MG inoculation or had been inoculated with S6MG at either 10 or 22 wk of age. Alterations in eggshell quality in response to S6MG may become evident only in older birds that are experiencing declines in production when housed under controlled conditions.

Aging↗

A comparison of fitting growth models with a genetic algorithm and nonlinear regression.

A genetic algorithm (GA), an optimization procedure based on the theory of evolution, was compared with nonlinear regression for the ability of the 2 algorithms to fit the coefficients of poultry growth models. It was hypothesized that the nonlinear approach of using GA to define the parameters of growth equations would better fit the growth equations than the use of nonlinear regression. Two sets of growth data from the literature, consisting of male broiler BW grown for 168 and 170 d, were used in the study. The growth data were fit to 2 forms of the logistic model, the Gompertz, the Gompertz-Laird, and the saturated kinetic models using the SAS nonlinear algorithm (NLIN) procedure and a GA. There were no statistical differences for the comparison of the residuals (the difference between observed and predicted BWs) of growth models fit by a GA or nonlinear regression. The plotted residuals for the nonlinear regression and GA-determined growth values confirmed observations of others that the residuals have oscillations resembling sine waves that are not represented by the growth models. It was found that GA could successfully determine the coefficients of growth equations. A disadvantage of slowness in converging to the solution was found for the GA. The advantage of GA over traditional nonlinear regression is that only ranges need be specified for the parameters of the growth equations, whereas estimates of the coefficients need to be determined, and in some programs the derivatives of the growth equations need to be identified. Depending on the goal of the research, solving multivariable complex functions with an algorithm that considers several solutions at the same time in an evolutionary mode can be considered an advantage especially where there is a chance for the solution to converge on a local optimum when a global optimum is desired. It was concluded that the fitting of the growth equations was not so much a problem with the fitting methodology as it is with the form of the equation.

Algorithms↗

Growth responses of male broilers subjected to increasing air velocities at high ambient temperatures and a high dew point.

This study examined live performance responses of male broilers to increasing air velocity of 120 and 180 m/min reared under high cyclic temperatures (25-35-25 degrees C) with a 23 degrees C dew point from 21 to 49 d. Birds were reared in an environmental facility containing 2 wind tunnels (4 pens/tunnel) and 6 floor pens (control). At 21 d, 53 birds were placed in each pen of the wind tunnels and control group, respectively, and growth performance was determined weekly. Increasing air velocity from 120 to 180 m/min improved BW and BW gain from 29 to 35, 36 to 42, and 43 to 49 d of age leading to a cumulative advantage of 287 g in BW gain and a 10-point difference in feed conversion from 21 to 49 d of age. Subjecting birds to air velocity improved growth rate, feed consumption, and feed conversion at each weekly interval from 28 to 49 d over the control birds. These results indicate that male broilers approximating 2.0 to 3.0 kg respond to an air velocity of 180 m/min when exposed to high cyclic temperatures.

Air Movements↗

Live performance of male broilers subjected to constant or increasing air velocities at moderate temperatures with a high dew point.

This study examined the effects of varying air velocities vs. a constant air velocity with a cyclic temperature curve of 25-30-25 degrees C and a dew point of 23 degrees C on broilers from 28 to 49 d of age. Four replicate trials were conducted. In each trial, 742 male broilers were randomly allocated to 6 floor pens or 2 air velocity tunnels, with each tunnel consisting of 4 pens. Bird density, feeder, and waterer space were similar across all pens (53 birds/ pen; 0.07 m2/bird). The treatments were control (still air), constant air velocity of 120 m/min, and increasing air velocity (90 m/min from 28 to 35 d, 120 m/min from 36 to 42 d, and 180 m/min from 43 to 49 d). Birds grown in a still air environment gained less weight, consumed less feed, and converted feed less efficiently between 28 and 49 d than birds subjected to moving air (constant or increasing). Growth responses between the air velocity treatments were similar from 28 to 35 and 36 to 42 d of age. Increasing air velocity to 180 m/min improved (P < or = 0.02) the growth rate of broilers from 43 to 49 d of age over birds receiving an air velocity of 120 m/min, but the incidence of mortality was not affected. These results provide evidence that increasing air velocity from 120 to 180 m/min is beneficial to broilers weighing 2.5 kg or greater when exposed to moderate temperatures.

Air Movements↗

Stocking density effects on growth performance and processing yields of heavy broilers.

This study examined responses of male broilers during a 49-d production cycle to 4 placement densities in 2 trials. Trials were pooled because no treatment x trial interaction occurred. In each trial, 1,488 male chicks were randomly placed into 32 floor pens to simulate final densities of 30 (37 chicks/pen), 35 (43 chicks/ pen), 40 (50 chicks/pen), and 45 (56 chicks/pen) kg of BW/m2 of floor space based on a projected final BW of 3.29 kg. Growth rate and nutrient utilization were similar (P > or = 0.05) among the treatments from 1 to 32 d of age. From 1 to 49 d, BW gain (P = 0.011) and feed consumption (P = 0.029) were adversely affected by increasing the placement density from 30 to 45 kg of BW/m2 of floor space. The reduction in cumulative BW gain due to placement density can be partially explained by less feed consumption as evidenced by 95.4% of the sums of squares of BW gain being attributable to feed consumption. Litter moisture content (P = 0.025) and foot pad lesion score (P = 0.001) increased linearly with increasing placement density. Upon processing, whole carcass and breast meat yields relative to BW were not affected (P > or = 0.05) as density increased from 30 to 45 kg/m2. The proportion of whole carcasses with scratches, but not tears, on the back and thighs increased (P = 0.021) as density increased. These results indicate that increasing the density beyond 30 kg/m2 elicited some negative effects on live performance of heavy broilers.

Animal Husbandry↗

A self-propelled, constant-speed spray vaccinator for commercial layer chickens.

Vaccination of commercial layer chickens is labor intensive and often results in poor rates of seroconversion, which, in turn, generally correlate with decreased flock uniformity and performance. Attempts to improve the vaccination process include numerous variations of individual shop-built vaccinators in use by the layer sector of the poultry industry. Each of these vaccinators has limitations that contribute to poor vaccinations. Major problems include the nonuniform speed of the applicator system and pressure fluctuations at the spray nozzles, which contribute to sporadic dispersion of the vaccine as the vaccinator is pushed or carried past the cages. A battery-powered, self-propelled, constant-speed vaccinator was designed and constructed that operates with constant nozzle pressure. In field use, this vaccinator has resulted in both labor savings (reduction of manpower from five to one to vaccinate 75,000 chickens) and time savings (from 45 min to 7.5 min/poultry house) as well as improved vaccination results (higher positive seroconversion rates) against the poultry pathogen Mycoplasma gallisepticum (MG), a bacterium associated with losses of 15.7 eggs/hen over a 45-wk laying period in MG-infected layers as compared with layers maintained free from infection with MG.

Animal Husbandry↗

Effects of fasting on serum lipids and lipoprotein profiles in the egg-laying hen (Gallus domesticus).

The effects of a 24-h fast on serum lipids and lipoprotein profiles in commercial laying hens were investigated. Blood was analyzed at 34 and 46 weeks of age from Single Comb White Leghorn hens that had been either fed ad libitum or had been fasted for 24 h prior to collection. At 12 weeks, birds were divided into 16 biological isolation units, with 8 replicate units assigned to each treatment group. Four birds out of 10 in each unit were tagged for bleeding. Parameters evaluated included total serum cholesterol and triglycerides, mean diameters of very low density lipoproteins (VLDLs) for the 10th, 50th, and 90th percentiles of serum total VLDL, mean total population VLDL particle diameter (MPD), and percentage serum cholesterol recovered in VLDL, low density lipoprotein (LDL), and high density lipoprotein (HDL) fractions. Fasting led to decreases in total serum cholesterol and triglycerides, and a decrease in mean serum VLDL particle diameter in the 90th population percentile. At Week 34, percentage serum cholesterol recovered from LDL was increased, whereas percentage serum cholesterol recovered from HDL was decreased due to fasting. At Week 46, MPD and percentage serum cholesterol recovered from VLDL were decreased, whereas percentage serum cholesterol recovered from HDL was increased due to fasting. It was concluded that a 24-h fast decreased serum lipids (cholesterol and triglycerides) and the size of VLDL particles in the 90th population percentile in commercial laying hens. Furthermore, bird age influenced the effects of a 24-h fast on MPD and the redistribution of serum cholesterol among VLDL, LDL, and HDL particles.

Animals↗

Atmospheric ammonia is detrimental to the performance of modern commercial broilers.

Atmospheric ammonia inhibits broiler performance. Quantified effects are based on older genetic stock with a BW of 2000 g at 7 wk. In contrast, modern genetic stock reaches 3200 g at 7 wk of age. To assess the impact on present day broilers, 2 trials were conducted exposing male broilers to graded levels (0, 25, 50, and 75 ppm) of aerial ammonia from 0 to 4 wk of age. Sixty, 1-d-old chicks were placed in environmentally controlled chambers, weighed weekly as a group, and processed with yield determined at 7 wk of age. Final BW was significantly depressed by 6 and 9% for the 50 and 75 ppm concentrations of ammonia as compared with 0 ppm. Also, mortality was significantly greater at the 75 ppm ammonia concentration, 13.9% compared with 5.8% for the 0 ppm treatment. Percentage yield of deboned meat per bird decreased slightly with increasing exposure to ammonia but was not statistically significant. Although current genetic stock reaches growout weights that are approximately 60% greater than those 2 decades ago, the relative quantified effects of ammonia exposure were similar. Additionally, statistical analysis of the results provided a simple equation, presented herein, for predicting the decline in BW of male broilers after exposure to ammonia.

Ammonia↗

Optimization of phase feeding of starter, grower, and finisher diets for male broilers by mixture experimental design: forty-eight-day production period.

A mixture experiment, a variant of response surface methodology, was designed to determine the proportion of time to feed broiler starter (23% protein), grower (20% protein), and finisher (18% protein) diets to optimize production and processing variables based on a total production time of 48 d. Mixture designs are useful for proportion problems where the components of the experiment (i.e., length of time the diets were fed) add up to a unity (48 d). The experiment was conducted with day-old male Ross x Ross broiler chicks. The birds were placed 50 birds per pen in each of 60 pens. The experimental design was a 10-point augmented simplex-centroid (ASC) design with 6 replicates of each point. Each design point represented the portion(s) of the 48 d that each of the diets was fed. Formulation of the diets was based on NRC standards. At 49 d, each pen of birds was evaluated for production data including BW, feed conversion, and cost of feed consumed. Then, 6 birds were randomly selected from each pen for processing data. Processing variables included live weight, hot carcass weight, dressing percentage, fat pad percentage, and breast yield (pectoralis major and pectoralis minor weights). Production and processing data were fit to simplex regression models. Model terms determined not to be significant (P > 0.05) were removed. The models were found to be statistically adequate for analysis of the response surfaces. A compromise solution was calculated based on optimal constraints designated for the production and processing data. The results indicated that broilers fed a starter and finisher diet for 30 and 18 d, respectively, would meet the production and processing constraints. Trace plots showed that the production and processing variables were not very sensitive to the grower diet.

Animal Feed↗