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Biomedical subjects

S L Fubini

Publications and source records attributed to S L Fubini.

At least 37 records · Page 2Linked to original sources

Right displacement of the abomasum and abomasal volvulus in dairy cows: 458 cases (1980-1987).

Clinical and surgical findings in 458 dairy cows with right displacement of the abomasum or abomasal volvulus (AV) were analyzed to determine the association between these variables and the outcome (productive, salvaged, or terminal) of the affected cow. Heart rate at initial examination was higher in the salvaged and terminal groups of cows (P less than 0.0001) than in the productive group. The plasma Na+ concentration was lower in the salvaged and terminal groups of cows (P less than 0.0001) than in the productive group. The plasma C1- concentration was lower in the salvaged group of cows than in cows with either productive or terminal outcomes (P less than 0.0001). Anion gap was significantly higher (P less than 0.0001) in the salvaged and terminal groups than in cows classified as productive. On the basis of surgical findings, cattle with right displacement of the abomasum were more likely to survive than cows with AV. Cows that required fluid decompression of the abomasum via abomasotomy were less likely to survive than those in which gaseous decompression was performed. Cows with evidence of vascular compromise of the abomasal wall were less likely to survive than those with normal abomasal serosa. Preoperative heart rate and anion gap had positive correlations with the presence of abomasal necrosis, whereas temperature, venous blood pH, plasma bicarbonate, and base excess concentrations had negative correlations. Cows in terminal stages of AV were likely to have a mixed primary acid-base disturbance, consisting of metabolic alkalosis with superimposed metabolic acidosis. These findings were highly correlated with abomasal necrosis.

Abomasum↗

Replacement of chloride deficit by use of 1.8% NaCl to correct experimentally induced hypochloremic metabolic alkalosis in sheep.

Five adult 40- to 50-kg female sheep were surgically fitted with a reentrant cannulae placed in the proximal part of the duodenum just distal to the pylorus. By diversion of abomasal outflow, this model has been shown to produce hypochloremic metabolic alkalosis accompanied by dehydration, hypokalemia, and hyponatremia. Each sheep was subjected to 3 separate, 12-hour IV treatment trials, in each case preceded by a control period of 48 hours, and a diversion period of 36 to 96 hours, during which a hypochloremic (Cl- less than or equal to 60 +/- 2 mEq/L) metabolic alkalosis with hypokalemia and hyponatremia was produced. Treatment 1, consisting of 6 L of isotonic Na gluconate, was designed to replace volume without replenishing the Cl-1 deficit. Although hydration improved, plasma Cl- decreased further, and the sheep became increasingly weak and depressed. Treatment 2, consisting of 2 L of 1.8% NaCl, was designed to replace the Cl- deficit without replacing total volume. Plasma Na+ and Cl- concentrations returned to normal during the 12 hours of treatment; acid-base balance and plasma K+ concentrations returned to normal within 36 hours of treatment. During treatment 3 (control, no treatment), measured metabolic values changed minimally. We concluded that the IV replacement of Cl- without K+ is effective in the correction of experimentally induced hypochloremic metabolic alkalosis in sheep.

Alkalosis↗

Formation of N-trimethyl-N-nitrosourea in the gastric lumen of the fistulated pig.

The formation of N-trimethyl-N-nitrosourea (TMNU) in the stomach of full-sized gastric-fistulated pigs was determined. Nitrite was added to the stomach in amounts that have been reported to occur in the human stomach (25-375 mumol). The vehicle was an 'artificial meal' made up of synthetic gastric juice. The total amount of TMNU formed (not concentration) was estimated by determining the concentration and gastric volume using multiple additions of a nonabsorbable marker (polyethylene glycol). At an initial pH of 3, 23-1000 micrograms of TMNU were found after 10-25 min. The rate of loss of polyethylene glycol and TMNU from the stomach were the same, indicating that TMNU was not directly absorbed. These data suggest that formation of TMNU under conditions similar to those found in the human stomach is possible.

Animals↗

Accuracy of radiography of the reticulum for predicting surgical findings in adult dairy cattle with traumatic reticuloperitonitis: 123 cases (1981-1987).

Radiographic and surgical findings were compared in 123 cattle suspected of having traumatic reticuloperitonitis. Radiography of the reticulum proved to be a sensitive test for detection of a foreign body (FB). An abnormal FB position on a radiograph was a good predictor of FB perforation. If an FB was fully attached to a magnet, it was unlikely to be perforating the reticular wall. When abnormal reticulum size, abnormal reticulum location, and gas shadows adjacent to the reticulum were found simultaneously on a radiograph, hepatic or perireticular abscess was likely. Reticular radiography proved to be a useful diagnostic aid in cattle suspected of having traumatic reticuloperitonitis.

Animals↗

Surgery of the bovine large intestine.

Cecal dilatation and volvulus are common entities in dairy cattle, whereas cecal torsion is not often encountered. These diseases have been related to excessive volatile fatty acid production. Obstruction of the remainder of the large colon may be simple or strangulating in nature. These obstructions are relatively rare. Clinical signs, surgical management, and surgical complications of diseases of the cecum and large intestine are discussed.

Animals↗

Surgery of the neonatal bovine digestive tract.

Many disorders of the calf's gastrointestinal tract require surgical intervention if a successful outcome is to be obtained. The most common abnormalities in this category are abomasal volvulus, abomasal ulcers, small intestinal accidents, and atresia of the spiral colon. These can be differentiated by the age of the animal at presentation and a careful physical examination. Special considerations in neonatal gastrointestinal surgery include: ensuring adequate serum immunoglobulin status, rapid treatment of dehydration and hypoglycemia, and consideration of the inheritability of any corrected defects. Prompt attention to metabolic disturbances and correction of the abnormalities are essential for a successful outcome.

Animals↗

Quantitative estimates of N-nitrosotrimethylurea formation in the porcine stomach.

Gastric formation of N-nitrosotrimethylurea (NTMU) was quantified by repeated sampling of the gastric contents of full-sized fistulated pigs after the addition of 250 mumol trimethylurea (TMU) and 25-125 mumol nitrite. Maximum gastric nitrite concentration was 100-500 microM based on the gastric volume and amount of added nitrite, which is within the range reported in human gastric contents. The total amount of NTMU formed over time was quantified by estimating both the NTMU concentration and gastric volume using multiple additions of polyethylene glycol. Administration of 25 mumol of nitrite resulted in the formation of 320 nmol of NTMU. The total amount of NTMU formed was linearly related to nitrite dose. Conversion of nitrite to NTMU was of the order of 1-3%. Potassium thiocyanate (200-2000 mumol) had no effect on NTMU formation when given immediately preceding 75 mumol nitrite. Ascorbic acid at 225 and 341 mumol (0.66 and 1.0 of the recommended dietary allowance) inhibited nitrosation of TMU by an average of 54 and 84%. A pig with an average gastric pH of 1.9 formed 4.5-fold more NTMU than did a second pig at pH 4.8. These results suggest that low micromolar amounts of N-nitrosoureas can be formed in the normal stomach when nitrite is consumed in amounts to which humans are commonly exposed.

Animals↗

Use of a multiple logistic regression model to determine prognosis of dairy cows with right displacement of the abomasum or abomasal volvulus.

Data at admission and at surgery were collected on 458 cows with right displacement of the abomasum or abomasal volvulus, to derive multiple logistic regression models for predicting postsurgical outcome (productive, salvaged, or terminal). The derived models contained few and easily obtained variables. The weight associated with each variable was determined objectively. Three admission variables (heart rate, base excess, and plasma chloride concentration), and 5 surgical variables (heart rate, base excess, diagnosis, method of decompression used, and appearance of abomasal serosa) were used in the final models. Predicted outcomes that used the admission and surgical models were closely related with actual outcomes. Total correct classification for satisfactory (productive) versus unsatisfactory outcome (salvaged and terminal) was 78.2% for the admission model and 82.7% for the surgical model. Combining data on cows with productive and salvaged outcomes as satisfactory outcome, and terminal as unsatisfactory outcome, total correct classification was 90.7% for the admission model and 93.2% for the surgical model. Using predicted probabilities, the market value of productive and salvaged cows, and the medical and surgical costs, one can calculate the expected economic value of each outcome. Treatment can be justified if the sum of the expected value of productive and salvaged outcome exceeds the sum of the medical and surgical costs and the expected salvaged value of the cow that was not treated surgically.

Abomasum↗

Failure of omasal transport attributable to perireticular abscess formation in cattle: 29 cases (1980-1986).

During a 7-year period, failure of omasal transport attributable to a perireticular abscess was diagnosed in 29 cows. Affected cattle were examined because of anorexia, hypogalactia, and bilateral abdominal distention. The cows were all female Holsteins, 15 months to 10 years old. The abscess was identified during exploratory celiotomy and rumenotomy or at necropsy. Traumatic reticuloperitonitis was believed to be the cause. Twenty-seven cattle (93%) were treated surgically. The abscess was drained into the reticulum or omasum in 25 cows (86%). Twenty-four cows (83%) survived to the time of discharge from the hospital, and 20 (69%) survived for at least 1 year and became productive members of the herd. This is a better survival rate than that reported for other causes of vagal indigestion.

Abscess↗

Pyloric obstruction after toggle-pin fixation of left displaced abomasum in a cow.

One potential complication of blind abomasopexy techniques, including the toggle-pin technique, is the possibility of creating pyloric outflow obstruction. Cows that have had left displaced abomasum corrected by this method should be observed closely for the first 48 postsurgical hours for signs of deterioration. If pyloric obstruction is suspected and the securing sutures are cut before fibrous adhesions have formed, permanent damage may be minimized.

Abomasum↗

Fistulous withers in horses: 14 cases (1978-1987).

Over an 8-year period, 14 horses were admitted to the New York State College of Veterinary Medicine for evaluation of fistulous withers. Of the 14 horses, 11 were treated surgically and 3 were not treated. Surgical techniques involved radical excision of the affected soft tissue and spinous processes of affected vertebrae. Recurrence rate was 30%, and these horses required additional surgery to resolve the problem.

Animals↗

Attempts to prevent equine post neurectomy neuroma formation through retrograde transport of two neurotoxins, doxorubicin and ricin.

Digital neurectomies, performed to relieve pain and lameness, are often complicated postoperatively by formation of painful neuromas. In this study attempts were made to deliver lethal doses of neurotoxin to the cell bodies of the transected digital nerve fibres via long-distance retrograde axon transport and, thereby, prevent the regenerative changes that lead to neuroma formation. After applying doxorubicin in various ways to the digital nerve stumps of ponies, degenerating or necrotic neurones appeared only sporadically in the spinal ganglia. Although doxorubicin was largely ineffective in retrograde destruction of cell bodies, when absorbed in pledgets on the stumps it exerted a sustained action which prevented Schwann cell proliferation and axon sprouting. Ricin, in contrast to doxorubicin, was effective in retrograde destruction of sensory neurons. Many affected neurons were devoid of polysomes but packed with mitochondria; others had advanced to various stages in cytolysis. Despite its effectiveness, ricin cannot be recommended because of its extreme toxicity. The clinical use of retrograde transport in equine neurectomy will probably depend on future development of hybrid toxins with high neural specificity and low systemic toxicity.

Animals↗

Urinary bladder rupture in a two-year-old horse: sequel to a surgically repaired neonatal injury.

After routine cryptorchid castration, a 2-year-old Thoroughbred colt was admitted 72 hours later because of depression, abdominal distention, and pollakiuria, with production of small quantities of urine. A diagnosis of a ruptured bladder was made on the basis of a large volume of abdominal fluid and a disparity between the urea nitrogen and creatinine concentrations in the serum (70 mg/dl and 8.4 mg/dl, respectively) and in the abdominal fluid (154 mg/dl and 43 mg/dl, respectively). The colt had undergone surgical correction of a ruptured urinary bladder at 4 days of age, and a 5-cm tear through one of the previous scars was identified and repaired during exploratory celiotomy. The previous injury to the bladder was extensive and may have left an inherent weakness in the bladder wall. Evidence of adhesion formation or urethral obstruction was not found. The combination of a full bladder and the trauma associated with induction of anesthesia may have contributed to the recurrence of bladder rupture.

Animals↗

Cecal volvulus in a dairy cow: partial resection of the proximal portion of the ascending colon.

Cecal volvulus in a dairy cow with vascular compromise of the proximal portion of the ascending colon was corrected surgically with preservation of the ileocecocolic junction. Partial cecectomy was combined with resection of the proximal portion of the ascending colon and anastomosis of the remaining cecum to the distal portion of the ascending colon. The procedure was done with the use of mechanical suturing devices while the cow was standing. Previously, general anesthesia and relocation of the ileocecocolic junction had been suggested.

Animals↗

Abomasal fistulas in dairy cows.

Abomasal fistulas were corrected surgically in 9 adult Holstein cows. Six cows had a history of right paramedian abomasopexy (RPA), and 2 cows had had a percutaneous abomasopexy using the blind-stitch technique (BSA). Fistulas developed from 2 weeks to 12 months after the RPA or BSA. In 1 cow, history was not available beyond 2 years, and no surgery had been performed in that time. All fistulas were in the right paramedian area and were draining blood and/or ingesta from necrotic abomasal tissue (8 cows) or ruminal tissue (1 cow). Surgery was performed immediately in cows with consistent hemorrhage from the fistulated tissue (4 cows). Surgery was delayed to reduce rumen contents if bleeding was absent or considered negligible (4 cows) or to stabilize the patient (1 cow). General anesthesia was used in 7 cows, local anesthesia in 2 cows. Outcome following the fistula resection was successful in the 7 cows discharged from the clinic. The occurrence of abomasal fistulation is probably related to nonabsorbable sutures penetrating the abomasal lumen during the abomasopexy procedure resulting in infection of the incision or body wall.

Abomasum↗

Cecal dilatation and volvulus in dairy cows: 84 cases (1977-1983).

The records of 84 cows with cecal dilatation or cecal volvulus were reviewed. The diagnosis was made by exploratory laparotomy or necropsy in 21 cows with cecal dilatation, and in 45 cows with cecal volvulus. Eighteen of the cows with cecal dilatation responded to medical management and the diagnosis was made by physical examination alone. The most useful criteria for determining that surgery was necessary were the presence of a high heart rate, apparent abdominal pain, scant or absence of feces, and the apex of the cecum rotated cranially on rectal examination. The prognosis for cattle with cecal dilatation and volvulus was good following medical therapy or decompression at surgery unless there was severe vascular compromise of the cecum. There was a 10% recurrence rate, and in these cows a partial typhlectomy was performed.

Animals↗

Tracheal rupture in two horses.

Two horses were admitted for evaluation of diffuse SC emphysema. Diagnosis of ruptured trachea was made by tracheoscopy and radiography. The tracheal cartilages were not involved in either case. One of the defects was small and responded to medical management. The other defect was large and was apposed surgically. Both horses recovered without complications.

Animals↗