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S Lafitte

Publications and source records attributed to S Lafitte.

42 records · Page 3Linked to original sources

[Comparison of echocardiographic automatic border detection and magnetic resonance imaging. Measurements of the left ventricle in normal subjects].

Echocardiographic automatic border detection (ABD) has been the object of several studies with diverging results. The aim of this study was to verify the validity of ABD measurements by comparison with magnetic resonance imaging (MRI). Twenty healthy subjects underwent measurement of end systolic surface (ESA) and end diastolic surface areas (EDA) and fraction of surface variation (FSV), end systolic volume (ESV), end diastolic volume (EDV and ejection fraction (EF). These results were compared with the same parameters measured by cine MRI and a study of the variability of interpretation was performed on the echocardiographic parameters. An ABD analysis was possible in 80% of the study population. The correlations were satisfactory between the EDA and EDV (EDA; r = 0.84; SD = 1.9 cm2; EDV; r = 0.90; SD = 12 ml) with acceptable confidence intervals (CI) (EDA; [-4.02; 1.19 cm2]/EDV; [26; +7.9 ml]) and an underestimation of ABD values (EDA; -9%/EDV: -10%). With regards to the end systolic measurements, the correlations were not as good (ESA: r = 0.68; SD = 1.5 cm2/EDV: r = 0.59; +12 ml) with a more important measurement error (ESA: -2.05; +3.45 cm2)/EDA: (-9; +27 ml) and an overestimation of the ABD values (ESA; +10%; ESV; +18%). No correlation was observed between the FSV and EF. The intra and inter-observer errors were compared with those of conventional echocardiography (intra-observer error; 10.7-16.9%/inter-observer error; 10.8-16.6%). The authors conclude that ABD has a non-negligeable measurement error which limits its application in clinical practice. New transducers, automatisation of gain adjustment and new technologies should improve ABD measurements.

Adult↗

[Contrast echocardiography and myocardial perfusion: what is the current status?].

Progress in the field of echocardiographic contrast agent combined with progress in imaging techniques (second harmonic imaging, intermittent imaging, Doppler Energy) should allow a real revolution in the field of noninvasive cardiac imaging, and one of the main advantages will probably be myocardial perfusion imaging in ischaemic heart disease.

Contrast Media↗

Expression of a Schistosoma mansoni 28-kilodalton glutathione S-transferase in the livers of transgenic mice and its effect on parasite infection.

Schistosomiasis is a debilitating tropical disease for which an effective vaccine is needed. A 28-kDa glutathione S-transferase from Schistosoma mansoni (Sm28GST) has been shown to induce protective immunity. Sm28GST possesses significant sequence identity to mammalian GST isoforms. In order to study self-reactivity in mice immunized with Sm28GST and the concomitant phenomena of immune tolerance and epitope suppression, as well as their consequences for the protective immunity induced by this vaccination, we developed transgenic (Tg) mice that express Sm28GST under the control of a part of the mouse transferrin gene promoter. A study of (P28)Tg mice showed that the expression of Sm28GST was strictly localized in pericentrolobular hepatocytes. No histological change, inflammatory infiltrates, or modification of seric L-aspartate: 2-oxoglutarate aminotransferase concentration was observed over an 18-month period, despite a cross-reactivity between Sm28GST and a mouse molecule of 30 kDa. The immunoglobulin G anti-Sm28GST response of (P28)Tg mice immunized with recombinant Sm28GST was lower (P < 0.001) than that observed in non-(P28)Tg littermates and inversely proportional of Sm28GST liver expression. The response of non-(P28)Tg mouse spleen cells to Sm28GST stimulation was greater (P < 0.01) than that observed with (P28)Tg mouse spleen cells. (P28)Tg mice infected with 40 S. mansoni furcocercariae harbored more worms (P < 0.05) than did non-(P28)Tg control mice. The increase in the level of infection in (P28)Tg mice was reflected in concomitant increases in the numbers of adult worms and schistosome eggs found in livers and intestines after whole-body perfusion at 56 days postinfection, but no relative increase in the fertility of individual female worms was observed. The results obtained argue for the involvement of Sm28GST in reducing levels of infection and support the view that this enzyme has a central role in the maintenance of parasite viability, at least during its migration through host tissues.

Animals↗

[Role of echocardiography in the diagnosis of acquired diseases of the thoracic aorta].

The diagnosis and follow-up of acquired thoracic aortic disease have greatly improved with advances in transthoracic and transoesophageal echocardiographic techniques. In emergency situations, transoesophageal echocardiography is the key diagnostic investigation for dissection, significantly speeding up surgical referral. Atherosclerosis of the aorta is the second clinical situation in which transoesophageal echocardiography confirms its superiority over other imaging techniques for the recognition of intra-aortic debris carrying a high embolic risk.

Adult↗

Immunoglobulin A response in murine schistosomiasis: stimulatory role of egg antigens.

The immunoglobulin G (IgG) and IgA antibody responses to different Schistosoma mansoni antigens have been determined in chronically infected mice as well as in unisexually infected animals. With a panel of enzyme-linked immunosorbent assays (ELISAs), soluble antigens from furcocercariae, adult worms, and eggs were probed with sera collected at 3-week intervals. Bisexually infected animals developed significant IgG and IgA antibody responses to the antigens tested, which increased after egg deposition. In unisexual infections no significant differences were recorded in the IgG antibody profile for furocercaria and adult worm antigens, whereas the IgA antibody response was impaired. Both the IgA and IgG antibody responses toward egg antigens were reduced compared with those in a bisexual infection. Furthermore, a specific mucosal IgA antibody response was observed only in the bisexually infected animals. Histological analysis performed on bisexually infected mice led to the observation of eggs and granulomatous lesions within the Peyer's patch follicles, which are essential sites for the induction of mucosal immunity in the intestine. These data suggest a relationship between egg deposition and the induction of the IgA antibody response toward schistosomes.

Animals↗