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Biomedical subjects

S Leeson

Publications and source records attributed to S Leeson.

At least 19 recordsLinked to original sources

Layer performance of four strains of Leghorn pullets subjected to various rearing programs.

Two experiments were conducted using four strains of Leghorn pullets, namely Babcock, DeKalb, H & N, and Shaver. Pullets were grown on conventional or low protein diets fortified with additional amino acids. At 18 wk of age, 64 pullets from each strain and diet treatment were transferred to individual laying cages, using eight replicate groups of four adjacently caged birds. In a second experiment, pullets from the four strains were selected based on body weight at 18 wk of age (approximately 1,270 vs 1,650 g). Each weight group and strain was again represented by eight replicate groups of four birds. In Experiment 1, there were no strain or rearing diet effects on egg production (P > 0.05). Rearing diet had little long-term effect on any adult characteristics. There were significant (P < 0.01) strain effects on body weight, feed intake, and egg weight, although these were independent of rearing diet. In Experiment 2, regardless of bird strain, the pullets with smaller body weight matured more slowly (P < 0.01) and produced less total egg mass to 70 wk age (P < 0.05). These smaller birds ate less feed and produced smaller eggs (P < 0.01). There were strain effects, independent of 18-wk body weight, for egg weight and eggshell quality (P < 0.01). It is concluded that minor strain differences exist with respect to response to juvenile nutrition, although such effects are only evident in early lay. All strains of bird remain small, 18-wk body weight is reduced, and these birds subsequently eat less feed and produce smaller eggs.

Aging

Influence of dietary levels of fat, fiber, and copper sulfate and fat rancidity on cecal activity in the growing turkey.

Two experiments were conducted with 6- to 10-wk-old turkeys. In Experiment 1, 6-wk-old turkeys were fed diets varying in level of fat (4.4 to 10%) or fiber (2.5 to 9.0%). The diets also contained extra copper as copper sulfate at either 0.1 or 0.2% of the diet. At 8 wk of age, 15 replicate birds were housed in individual cages and all excreta was collected. Excreta was separated as being "regular" or "cecal" in origin based on appearance. At 10 wk of age, 10 birds per treatment were killed and cecal contents removed under anaerobic conditions. Cecal contents were assayed for various nutrients and viscosity was measured. In a second comparable study, turkeys were fed animal-vegetable fat or regular or rancid canola oil (60.25 vs 120.24 ng/g malonaldehyde, respectively). In Experiment 1, feeding copper sulfate had the most noticeable effect on various cecal parameters. There was an increase (P < 0.01) in dry matter cecal droppings produced and the cecal contents were of increased viscosity (P < 0.05). Copper had no effect on pH or microbial colony count of the cecal contents. Feeding copper resulted in a significant increase in the high molecular weight (> 300,000) fraction of cecal contents and this fraction was of higher viscosity. Feeding copper sulfate resulted in a dramatic increase in copper content of cecal contents (280 to 11,848 ppm), although the copper content of regular excreta was also increased (17 to 1,008 ppm). The various levels of fiber and fat generally had no effect on cecal parameters. Feeding rancid canola oil did result in increased viscosity of cecal contents, compared to the situation seen with fresh canola oil. Fat rancidity per se, however, failed to influence other parameters such as total mass of cecal material produced and composition of cecal material. Feeding copper sulfate or rancid fat will increase the viscosity of cecal material, which may contribute to litter management problems.

Animals

A problem with characteristics of the thin albumen in laying hens.

Industry observations indicate that a proportion of shell eggs exhibit an uncharacteristic spreading of the thin albumen when they are broken-out for fast-food preparation. Because the height and characteristics of the thick albumen are unaffected, the eggs are classified as Grade A. Preliminary observations discounted effects of egg storage or contaminated feed. Two experiments were conducted with Leghorn hens over a full laying cycle, and involved different levels of protein (14, 16, 18, or 20%) and associated amino acids, or different degrees of acid-base balance (150, 200, 250, or 300 mEq/kg). In each trial, treatments were represented by eight replicate groups of four adjacently and individually caged birds. Diet treatment had some effects on conventional production parameters, although there were no major effects on the area of the thin albumen produced when eggs were broken out. In Experiment 1, birds fed the highest level of protein produced eggs with the smallest thin albumen area; however, there was considerable bird to bird variation with thin albumen area varying from 69 to 122 cm2. Ten birds with compact (x 69 cm2) and 10 birds with spreading thin albumen (x 112 cm2) were inseminated with semen from a single, unrelated rooster. Offspring from these hens had significantly (P < 0.01) different thin albumen characteristics corresponding to those of their dams. Offspring producing eggs with the spreading thin albumen had the thickest eggshells as assessed by deformation measurement (P < 0.05). It is proposed that the excessively large spreading thin albumen area is caused by eggs spending more time in the shell gland.

Acid-Base Equilibrium

Nutrition of the broiler chicken around the period of compensatory growth.

Three experiments were conducted with cage-reared broilers to 21 d following nutrient restriction from 6 to 12 d age. In Experiment 1, birds were full-fed from 6 to 11 or given 50% of ad libitum intake on a daily basis, or 100% of ad libitum intake on a daily basis when the diet was diluted 50% with oat hulls. Birds were not able to fully recover body weight depression by 21 d, although birds previously restricted, by whatever method, were more efficient (P < 0.01) in overall energy intake:body weight gain. Prior feed restriction had no effect on ability to metabolize diet energy (P > 0.05), although these birds did exhibit increased nitrogen retention compared to birds full-fed from 6 to 11 d. In a second experiment, birds were fed diets with 1.25, 1.38, 1.51, 1.63, 1.76, or 1.88% lysine in the realimentation diet from 12 to 21 d. Lysine level had no effect on growth rate or feed efficiency (P > 0.05) for full-fed birds; however there was a linear (P < 0.05) decline in growth rate from 12 to 21 d in response to extra dietary lysine for the birds previously feed restricted from 6 to 12 d. In a third experiment, birds were fed diets varying in energy (3,000 to 3,300 kcal/kg) or protein (22 to 29% CP) from 12 to 21 d following ad libitum vs 50% feed restriction from 6 to 11 d age. Protein level of the diet had little effect on performance traits to 21 d, although there was an indication of improved growth in response to the higher energy concentration. Birds full-fed from 6 to 11 d showed increased liver size at 21 d when fed more protein, although the converse was true for the restricted birds (P < 0.05). The growth response to diet energy was associated with increased carcass fatness. In general, there does not seem to be any advantage to manipulating diet formulation during realimentation of birds previously nutrient-restricted.

Abdomen

Broiler response to energy or energy and protein dilution in the finisher diet.

Two experiments were carried out with male broiler chickens in which diets from 35 to 49 d of age were diluted by oat hulls and sand. In one experiment only energy was diluted; in the second experiment, both energy and protein (amino acid) levels were affected. Each diet was tested with four replicate floor pens each containing 25 1-d-old birds. All birds received a standard starter diet to 16 d and then grower diet to 35 d. In Experiment 1, dilution of only energy resulted in a significant linear (P < 0.01) reduction in body weight at 42 d, although there was growth compensation after this time such that all birds weighed the same at 49 d. Diet energy dilution resulted in increased feed intake, although energy intake was not maintained (P < 0.01). Diet energy dilution generally had little effect on carcass weight or yield of breast meat, although there was less abdominal fat (P < 0.01). In Experiment 2, dilution of both energy and protein had a comparable effect on growth rate as described in Experiment 1; however, in this study there was a linear decrease in carcass weight and breast meat yield as the diet was diluted. These results suggest that the male broiler chicken can grow quite well on very low energy diets but that a period of at least 7 d is required for adjustment to feed intake. Even with compensatory increase in feed intake, however, the bird is unable to maintain its energy intake when fed such diluted diets.

Amino Acids

Broiler response to diet energy.

Male broiler chickens were fed corn-soybean diets providing 2,700, 2,900, 3,100 or 3,300 kcal ME/kg. In all experiments, each treatment was tested with three replicate groups of 30 birds grown to 49 d of age. In Experiment 1, birds consumed the various diets ad libitum whereas in Experiment 2, all birds received identical and restricted quantities of feed so as to ensure variable intakes of energy. In a third experiment, after 7 d of age, broilers had access to feed in two feeders that contained only the highest level of energy, or the 3,300 kcal ME/kg diet in combination with one of the other diets previously described. Providing diets of 2,700 to 3,300 kcal ME/kg for ad libitum consumption had no effect on growth rate (P > 0.05) and energy intake was constant; however, reducing the energy level of the diet did result in reduced carcass fatness (P < 0.01). When feed intake was controlled in Experiment 2, there was reduced growth (P < 0.01) rate as energy level of the diet was reduced. This reduced growth was associated with dramatic reduction in carcass fatness (P < 0.01), although breast meat yield was not affected. When broilers were offered a choice of diets, they showed remarkably precise control of intake, such that energy intake was again constant across all treatments. However, even though energy intake was constant, broilers consuming the choice diets involving the lower energy content diets tended to have less carcass fat. It is concluded that the broiler still possesses a good ability to control its feed intake based on desire to normalize energy intake. As energy intake is decreased, or there is increased protein intake, the bird deposits less carcass fat.

Animals

Changes in body composition and adipocyte cellularity of male broilers subjected to varying degrees of early-life feed restriction.

Three experiments were conducted to determine growth performance, body composition changes, and adipocyte characteristics of male broilers subjected to early-life feed restriction. The purpose of Experiment 1 was to determine 42-d growth performance and body composition changes. Treatments used were full-fed control and 50% physical feed restriction during the period 6 to 12 d of age. Experiment 2 was conducted to determine the adipocyte characteristics during and after feed restriction to 42 d of age. An additional treatment involved gradual refeeding following the feed restriction period. Experiment 3 was designed to test the effect of feeding different types of dietary fat during realimentation on the abdominal fat pad (AFP) size, and lasted until 18 d of age. Three types of fats were tested, namely animal-vegetable (A-V) blend, flax oil, and animal tallow, each represented by full-fed and feed-restricted treatments. Complete compensatory growth by feed-restricted birds relative to controls was not achieved by 42 d in either Experiments 1 or 2. Early feed restriction resulted in lower body fat percentage at 12 d (P < 0.05), although by 42 d a higher rate of fat deposition mainly by hypertrophy of adipocytes resulted in no difference in fatness. Gradual reintroduction into ad libitum feeding did not alter such adipocyte hypertrophy nor improve compensatory growth response during refeeding. There was consistent improvement in feed efficiency associated with early-life feed restriction (P < 0.05). The use of different types of dietary fat did not effect the AFP when expressed as percentage body weight. Birds that were fed A-V blend during the refeeding period had bigger AFP (grams per bird), a situation that is attributable to differences in body weight. Attainment of growth compensation by the feed-restricted broilers apparently requires a more prolonged growth period or a less severe feed restriction program than that used here. Saturation of dietary fat has no effect on realimentation characteristics of the broiler.

Adipocytes

Effect of dietary fat, fiber, and monensin on cecal activity in turkeys.

Two experiments were conducted to determine the physical, chemical, and microbial properties of turkey cecal droppings and relate them to intake of common dietary components, namely fat, fiber, and the anticoccidial, monensin. Experiment 1 involved collection and analysis of physical and chemical properties of cecal and regular droppings from commercial turkey flocks. Experiment 2 tested the effect of dietary fat, fiber, and monensin on growth performance and cecal activity in male turkeys. Compared to regular excreta, cecal droppings analyzed in Experiment 1 were higher in viscosity and fat content, and lower in dry matter, nitrogen, and fiber content (P < 0.05). High dietary fiber and fat significantly (P < 0.05) improved growth performance in Experiment 2. Prolonged feeding of monensin significantly (P < 0.05) reduced cecal evacuation. Results of chemical composition showed that higher dietary fat and fiber significantly (P < 0.01) reduced the fat percentage of cecal contents, whereas prolonged feeding of monensin increased the fat in both cecal contents and droppings. There was no significant effect of any of the treatments on pH, viscosity, and microbial counts of cecal contents. There is the need to identify and characterize the compound responsible for the high viscosity of cecal droppings.

Animals

alpha-Linolenic acid- and docosahexaenoic acid-enriched eggs from hens fed flaxseed: influence on blood lipids and platelet phospholipid fatty acids in humans.

This study was undertaken to examine the effects that consumption of eggs from hens fed diets containing flaxseed would have on plasma and platelet lipids of male volunteers. Feeding diets containing 0%, 10%, and 20% ground flaxseed to Leghorn pullets provided a marked progressive increase in n-3 fatty acid content as alpha-linolenic acid (alpha-LNA) (28, 261, and 527 mg/egg) and docosahexaenoic acid (DHA) (51, 81, and 87 mg/egg) but no alteration in the cholesterol concentration of the egg yolk. Twenty-eight male volunteers, divided into three groups, were fed four eggs per day for 2 wk according to a cyclic Latin-square design. No statistically significant changes were observed in total cholesterol, high-density-lipoprotein cholesterol, or plasma triglyceride concentrations. Significant increases in total n-3 fatty acids and in DHA content (which rose from 1.5 to 2.0% by wt or 33% overall), and a significant decrease in ratio of n-6 to n-3 fatty acids were found in platelet phospholipids of subjects consuming eggs from flaxseed-fed hens. Health and Welfare Canada in 1990 set recommended intakes for dietary n-3 fatty acids and for the ratio of n-6 to n-3 fatty acids, which are not being met currently by the overall population. Eggs modified by the inclusion of flaxseed in the laying hens' diet could provide an important nutritional source of n-3 fatty acid.

Adult

Relation between functional deficit and intraneural local anesthetic during peripheral nerve block. A study in the rat sciatic nerve.

BACKGROUND: During peripheral nerve block, local anesthetic (LA) penetrates within and along the nerve to produce the observed functional deficits. Although much is known about the kinetics and steady-state relation for LA inhibition of impulse activity in vitro in isolated nerve, little is known about the relation between functional loss and intraneural LA content in vivo. This study was undertaken to investigate the relation of functional change to intraneural LA. METHODS: A sciatic nerve block was performed in rats with 0.1 ml 1% lidocaine radiolabeled with 14C. The total intraneural uptake of LA was determined at different times after injection, and the distribution of lidocaine along the nerve was assayed at different stages of functional block. Drug content was also compared with equilibrium lidocaine uptake in the isolated rat sciatic nerve. RESULTS: Total intraneural lidocaine in vivo increased to near steady-state in about 3 min, stabilizing at approximately 14.3 nmol/mg wet tissue for about 12 min before decreasing to zero at 70 min after injection. Although intraneural lidocaine was 1.6% of the injected dose during full block, only 0.3% was left when deep pain sensation returned and 0.065% was still detected when functions fully recovered. Despite these large differences in total lidocaine content, the longitudinal distribution remained constant. Intraneural lidocaine concentrations obtained at full block and partial recovery could be achieved in vitro by equilibration in 0.7-0.9 and 0.2-0.3 mM lidocaine, respectively. CONCLUSIONS: During peripheral nerve block only a small amount of injected LA penetrates into the nerve. The intraneural content of LA correlates with the depth of functional block.

Animals

D-arabitol metabolism in Candida albicans: construction and analysis of mutants lacking D-arabitol dehydrogenase.

Candida albicans produces large amounts of the acyclic pentitol D-arabitol in culture and in infected animals and humans, and most strains also grow on minimal D-arabitol medium. An earlier study showed that the major metabolic precursor of D-arabitol in C. albicans was D-ribulose-5-PO4 from the pentose pathway, that C. albicans contained an NAD-dependent D-arabitol dehydrogenase (ArDH), and that the ArDH structural gene (ARD) encoded a 31-kDa short-chain dehydrogenase that catalyzed the reaction D-arabitol + NAD <=> D-ribulose + NADH. In the present study, we disrupted both ARD chromosomal alleles in C. albicans and analyzed the resulting mutants. The ard null mutation was verified by Southern hybridization, and the null mutant's inability to produce ArDH was verified by Western immunoblotting. The ard null mutant grew well on minimal glucose medium, but it was unable to grow on minimal D-arabitol or D-arabinose medium. Thus, ArDH catalyzes the first step in D-arabitol utilization and a necessary intermediate step in D-arabinose utilization. Unexpectedly, the ard null mutant synthesized D-arabitol from glucose. Moreover, 13C nuclear magnetic resonance studies showed that the ard null mutant and its wild-type parent synthesized D-arabitol via the same pathway. These results imply that C. albicans synthesizes and utilizes D-arabitol via separate metabolic pathways, which was not previously suspected for fungi.

Alleles

Response of broiler breeders to low-protein diets. 1. Adult breeder performance.

Four hundred female and 50 male commercial strain breeders were reared separately from 1 d to 18 wk of age, with all birds receiving the same diet. At 18 wk, all pullets were weighed and the extreme weights removed from the group. The remaining hens were then randomly sorted into four treatment groups each represented by four replicate groups. Treatment involved feeding different levels of CP (16, 14, 12, and 10%), supplemented with synthetic lysine and methionine in order to maintain constant lysine and TSAA levels, respectively. Diets were isoenergetic and all birds received the same quantity of feed daily. Dietary protein had no effect on egg production (P > .05). However, breeders fed 10% CP were lighter (P < .01) in weight than birds fed 16% CP. Eggs from birds fed 10 and 12% CP were consistently smaller (P < .01), and this resulted in reduced chick weight at hatching. Low-protein diets also resulted in less nitrogen excretion. These data suggest that it is possible to reduce the CP intake of broiler breeders while maintaining intake of critical amino acids without affecting performance.

Amino Acids

Response of broiler breeders to low-protein diets. 2. Offspring performance.

Broiler breeders were allocated at random to one of four experimental diets containing various levels of CP (16, 14, 12, or 10%), at constant methionine+cystine and lysine levels of .59 and .82%, respectively. Diets were isoenergetic and all birds received the same quantity of feed daily to 64 wk of age. Eggs from birds fed 10 and 12% CP were consistently smaller (P < .01) and in two trials involving breeders at 30 and 52 wk of age this resulted in reduced chick weight at hatching, although no lasting effect was observed on weight of offspring at 48 d. Better feed efficiency (P < .05) was observed from broilers hatched from hens fed lower CP diets. These data suggest that it is possible to reduce the CP intake of broiler breeders while maintaining intake of critical amino acids without adversely affecting offspring performance.

Animals

Utilization of fats and fatty acids by turkey poults.

Two experiments were carried out with young, Large White male turkey poults maintained in either floor pens or metabolism cages. In Experiment 1, poults were fed isoenergetic diets containing either no supplemental fat, or 5% of either tallow, corn oil, soybean oil, animal-vegetable blend fat, or canola oil. Poults generally ate less of the fat-supplemented diets and showed improved feed utilization, although weight gain was little affected. There was improved fat retention when vegetable oils were used (P < .01) and this was reflected in a slight improvement in diet energy level (P > .05). Poults fed tallow or animal-vegetable blend fat also excreted most fat. Diet had no effect (P > .05) on apparent retention of calcium or phosphorus, although retention of magnesium was less with more saturated fats. In Experiment 2, poults were fed diets containing palmitic acid, oleic acid, or a 50:50 (wt/wt) mixture of these fatty acids. There was a reduction (P < .05) in apparent retention of nitrogen, magnesium, calcium, and fat for poults fed palmitic acid, oleic acid, or the mixture. Mixing palmitic acid with oleic acid corrected some of these problems. However, reduced mineral retention was not reflected in any change in levels of bone ash, calcium, or phosphorus. Feeding palmitic acid did result in the most dramatic reduction of bone magnesium content (P < .05). It is concluded that turkey poults, like chicks, are less able to digest saturated fatty acids, and that such undigested fats can lead to reduced retention of some minerals through increased soap formation. However, there is no direct evidence that such soap formation causes a major change in bone calcium or phosphorus content or in gross bone development or poult well-being.

Animals

Effect of varying period of early nutrient restriction on growth compensation and carcass characteristics of male broilers.

An experiment was conducted to determine growth and carcass characteristics of male broilers subjected to varying periods of early nutrient restriction. Nutrient restriction was achieved by feeding a starter diet in which the major ingredients were replaced with 50% oat hulls. Five treatments involved feeding this diluted diet for 6 d, either continuous or split into shorter periods in different ways, all starting at 6 d of age. On completion of the 6 d of nutrient restriction, all birds received undiluted starter diet, followed by grower and finisher diets to trial termination at 49 d. All birds fed the diluted diet exhibited reduced nutrient intake. Complete growth compensation by all birds that previously received the diluted diet was attained by 35 d, due largely to their superior (P < .05) feed efficiencies relative to the control birds during realimentation. Varying the period of nutrient restriction did not affect growth compensation. Birds that received the diluted diet for 6 continuous d tended to be leaner at 42 d compared with those that consumed the diluted diet for shorter periods. This latter effect may relate to variable ME intake. There was no statistical difference in other carcass characteristics measured at both 42 and 49 d of age.

Animals

Strain comparison of turkey egg components.

Two experiments were conducted to quantify the composition of turkey hatching eggs in relation to strain of bird and age of breeder. In one experiment, 20 Nicholas and British United Turkeys of America (BUTA) eggs produced at 3, 12, and 24 wk of production were examined for physical and chemical components. Eggs from Nicholas hens were always some 4 g heavier (P < .05) due essentially to a larger albumen content. Although absolute components increased over time (P < .05), there were few changes in proportional composition (P > .05). Eggs from BUTA turkeys initially contained more yolk protein (P < .05). At 12 and 24 wk of production, eggs from Nicholas hens changed most rapidly and at these ages contained more yolk protein than the BUTA strain eggs (P < .05). At the end of the production cycle, BUTA eggs contained less lipid and protein in the yolk (P < .05). For both strains there was an increase in stearic acid content of yolk lipid with increased breeder age. In a second experiment, comparable studies were conducted on eggs obtained from male and female line BUTA breeding stock. Although male line eggs were heavier (87 vs 78 g), the smaller female line eggs exhibited proportionally more yolk (P < .05), as absolute yolk weight was similar for both strains (22.4 vs 22.5 g). It was concluded that only minor differences exist in components of eggs from different turkey strains.

Age Factors

Laying hen performance as influenced by protein intake to sixteen weeks of age and body weight at point of lay.

White Leghorn chicks were fed corn-soybean meal diets containing 20, 17, 14, and 11% protein, with similar levels of energy, from hatch to 16 wk of age. Body weights at 16 wk were similar for pullets fed the 20 and 17% protein diets, but were reduced by 11 and 27% for birds fed the 14 and 11% protein diets, respectively, as compared with the 20% protein diet. At 16 wk of age, all birds were placed on a common 17% protein laying diet. Pullets fed the 14 and 11% protein laying diets were slightly slower coming into production, however, by 28 wk of age egg production was similar for all four growing treatment groups and remained so until the end of the experiment. Average egg weight was similar for pullets fed the two higher levels of protein during the growing period and significantly lower for those pullets fed the 11% grower diet for all except the 28- and 32-wk periods. Pullets fed the 14% grower diet produced eggs with average weights significantly lower than those for the higher protein diets from 40 to 58 wk of age. Although the results might be interpreted as indicating that higher protein growing diets result in body protein reserves that subsequently enhance egg size, it is more likely that the pullets consuming lower protein diets produce smaller eggs because they have smaller body weights.

Age Factors