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Biomedical subjects

S Ling

Publications and source records attributed to S Ling.

48 records · Page 3Linked to original sources
PubMed1994

Culture shock in a university health service: a case and commentaries.

The detailed experience of one international student provides a case history in the use of college health services. His journey through the campus health center and outside referrals reveals important facets of student healthcare. It also offers some insights into foreign students' feelings as they contrast their health encounters with expectations carried from home. Four commentaries on the case attempt to draw inferences and to explain from various points of view what the student found in the course of seeking a diagnosis for rectal bleeding.

Adult↗
PubMed1992

Sister-chromatid exchange analysis in a rural population of Mexico exposed to pesticides.

Cytogenetic damage was evaluated by means of the analysis of sister-chromatid exchange (SCE) in a rural population of Tlaxcala, Mexico, in occupational contact with pesticides. We studied 170 men, 94 exposed and 76 not exposed. It was shown that SCE followed a normal distribution and Student's t test did not present differences between the two groups (P = 0.4). The frequency of SCE was not correlated with the duration of exposure of the rural workers (r = -0.06), the multiple covariance analysis applied to the data of duration of exposure, tobacco intake and alcohol ingestion demonstrated a lack of statistical significance. In the exposed people we observed no symptoms provoked by these compounds.

Adolescent↗
PubMed1990

Biochemical and immunochemical characterisation of strains of Treponema hyodysenteriae.

The protein composition of 18 clinical isolates of Treponema hyodysenteriae from pigs with swine dysentery in Australia were compared by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblot analysis. Coomassie Blue stained SDS-PAGE-profiles of whole cell and outer membrane (OM) proteins demonstrated the same gel pattern among the T. hyodysenteriae isolates, particularly the OM proteins in the molecular mass (Mr) range of 30 kDa to 40 kDa. The T. hyodysenteriae isolates were categorised into two distinct groups (A and B) based on the strain-variability in the 37 kDa OM protein. Immunoblotting of whole cell proteins after SDS-PAGE using serum from rabbits and pigs immunised with known T. hyodysenteriae serotypes revealed a number of common immunoreactive bands in all isolates. LPS typing of the T. hyodysenteriae isolates by immunoblotting with the rabbit antiserum revealed one additional serotype emphasising the LPS heterogeneity among the strains isolated from geographic locations in Australia, Great Britain and the U.S.A. Immunoblotting of the OM preparations revealed several common immunoreactive polypeptides corresponding to Mr values of 34 kDa to 30 kDa among the T. hyodysenteriae and T. innocens isolates but a distinct 39 kDa found only in the T. hyodysenteriae isolates. Trypsin proteolysis of intact. T. hyodysenteriae cells caused selective loss of these and other major abundant proteins identifying the location of the 39 kDa, 36 kDa, 34 kDa and 30 kDa proteins on the cell surface and suggesting a possible role of these proteins in the pathogenesis of swine dysentery.

Animals↗
PubMed1985

Intestinal membrane lipid composition and fluidity during development in the rat.

We have characterized microvillus membrane (MVM) lipid composition and derived estimates of membrane fluidity using fluorescence polarization [with 1,6-diphenyl-1,3,5-hexatriene (DPH) as the probe] in MVM from the jejunum and ileum of suckling (day 14-20) and mature postweaning (day 28-49) rats. The anisotropic values are lower (i.e., increased fluidity) in MVM from jejunum and ileum of suckling rats compared with values from MVM of mature rats, suggesting a more disordered molecular environment in MVMs of suckling animals, although the ileal MVM was relatively less fluid than jejunal membranes in both groups. Anisotropic determinations in protein-free MVM liposomes demonstrate that protein-lipid interactions are major determinants of fluidity estimates. Arrhenius plots of DPH anisotropies in MVM and MVM liposomes indicate a thermotropic lipid-phase transition at 23 degrees C in mature rat jejunum and ileum; however, no clear transition point was observed in 14- to 20-day animals. In addition, both the jejunal and ileal MVM in suckling animals contained greater amounts of total lipid, cholesterol, and phospholipid per milligram protein than did mature MVM preparations. Membrane phospholipid composition changed with age, with a decrease in sphingomyelin and an increase in phosphatidylcholine in the jejunum of the postweaning animal and with similar weight ratios of saturated to unsaturated fatty acids in both age groups. The data demonstrate that the small intestinal MVM manifests both age-dependent and regional proximal-distal differences in lipid composition and biophysical properties.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1975

Diet, growth and cultural food habits in Chinese-American infants.

This study was composed of one hundred Chinese-American mothers and infants in the Chinatown district of San Francisco. The mothers were interviewed with regard to infants' dietary intakes and past dietary patterns, infant health, maternal pre-natal history and diet, cultural habits, and socio-economic status. Infants' growth patterns were recorded and compared to those of American and Chinese babies. Their growth patterns were noted to be dissimilar to both American and Chinese growth patterns, but to be very similar to infant growth patterns in underdeveloped countries. The cultural influences on the dietary intakes were commented upon.

California↗