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Biomedical subjects

S M Amin

Publications and source records attributed to S M Amin.

11 recordsLinked to original sources
PubMed1992

A study on experimental cryptosporidiosis.

Cryptosporidium oocysts were isolated from stools of infected patients diagnosed as cryptosporidiosis. Suckling Swiss albino mice were infected orally by these oocysts in a dose of 10(6) oocysts/mouse. Mice were divided into 9 groups according to the duration of infection 2, 3, 4, 5, 6, 7, 10, 10, 15, 21 days postinfection, corresponding non infected control groups were included. Stools of infected mice were examined twice daily to determine; infection rate, prepatent period and intensity of infection. Mice were sacrificed according to the schedule and the whole intestine was isolated and processed for histopathological examination by H & E and modified Ziehl Nielsen stain, and histochemical examination for acid and alkaline phosphatase activity. The results revealed that the infection rate was 90% and prepatent period 3-5 days and the course of infection was 21 days. The maximum pathological changes were in the ileum. These changes were reversible.

Animals↗
PubMed1989

A trial to produce an anti-schistosomal vaccine using heterophyid antigens.

Heterophyid metacercariae, crude and partially purified heterophyid antigens were given prior to S. mansoni infections in an attempt to produce a potent antischistosomal vaccine. Three main groups of albino mice were used. Each group after receiving the appropriate heterophyid antigen prior to S. mansoni infection was studied parasitologically as regards worm load and tissue egg count and histopathologically as regards granulomata number, size and cellular constituents as well as immunologically by the indirect immunofluorescent test. The results showed a reduction in worm burden recoveries and egg load resulting in lesser number of granulomata and diminution in size of granulomata as well as acceleration in their reaction together with inhibition of fluorescence. These results were more obvious in the group infected one month post heterophyid infection, as well as in mice immunized with the highest dose of crude heterophyid antigen (200 micrograms protein). However, a low dose of 50 micrograms of partially purified fractionated antigen gave the most evident results.

Animals↗
PubMedDate not supplied

[Laboratory diagnosis and antibacterial therapy in joint diseases].

The indication for arthrocentesis is a difficult medical decision. Once obtained, however, puncture fluid is unique and important examination material which should always be thoroughly analyzed for diagnostic purposes, and also stored in a serum bank. In addition to the total protein and/or albumin fractions, the individual immunoglobulins in particular have proved to be a key parameter: they enable mechanical, hydro, inflammatory, and chronic-inflammatory processes to be distinguished. Especially in combination with the serum value, CRP is the best marker for acute inflammatory and traumatic changes. Determination of complement factors C1, C4, and C3, also of total complement activity, CH50 and of rheumatism factors, enables chronic-immunologic/rheumatic conditions to be diagnosed and their course evaluated. Bacteriologic study of punctates should always be included--not least as confirmation for the examiner--as also should cytologic and histologic examination of the sediment. Since, with bacterial etiology, the infections concerned are usually in their later stages, e.g., gonorrhea, yersinia, rubella, arthritis, antibody assays should always be performed in the serum and the joint in addition to these tests. Controversy still surrounds the question of local binding of immunoglobulin antibody complement components in the synovia itself; the present authors' findings hitherto also fail to furnish any definitive data on this issue.

Arthritis, Infectious↗