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S M Hunt

Publications and source records attributed to S M Hunt.

9 recordsLinked to original sources
PubMed1992

Induced expression of a Drosophila hsp70 promoter-fusion transgene is reduced after repeated heat shocks.

Levels of transcripts produced by a heat shock protein 70 (hsp70)-antisense white transgene in Drosophila were measured after single and multiple heat shocks to determine whether the hsp70 promoter could produce sustained high levels of transgene transcripts. A single heat shock resulted in typical highly inducible levels of RNA, but the amount of antisense RNA was substantially reduced after multiple heat shocks. Endogenous hsp70 mRNA levels were also less abundant after multiple heat shocks as compared to a single heat shock. The hsp70 promoter is unsuitable for use in fusion gene constructs for long term expression studies where repeated heat shocks are required.

Animals↗
PubMed1979

Hypnosis as obedience behaviour.

Obedience to authority has been implicated in hypnotic behaviour from the earliest theories. However, no exact formulation of a model of obedience was available until Milgram's experiments in the 1960s. Milgram's model can usefully be applied to hypnotic behaviour in terms of antecedent and immediate antecedent variables, binding and strain factors and the resolution of conflict. An experiment was carried out to test the hypothesis that the presence of a 'disobedient' hypnotic subject in the same room as another hypnotic subject would lower the susceptibility to hypnosis of the second subject. Results showed that when one subject disobeyed by leaving the hypnotic situation, susceptibility was significantly less than that of control subjects. It is suggested that hypnosis can be viewed as an 'agentic state' whereby the subject gives up autonomy and relinquishes responsibility for his actions to the hypnotist, whilst remaining responsible to the hypnotist for his performance as an hypnotic subject.

Adolescent↗
PubMedDate not supplied

Preservation of human granulocytes. III. Liquid preservation studied by electronic sizing.

Human granulocytes were isolated from blood either by counterflow centrifugation in a Beckman JE-6 rotor or by sedimentation of the red blood cells with dextran and centrifugation of the granulocyte-rich plasma. The stability of the granulocytes was assessed during storage in the liquid state by measurements of granulocyte loss, volume distribution characteristics, and ability to produce fluorescein in their cytoplasm and to exclude ethidium bromide from their nuclei. After storage at 4 C for 2 days in phosphate-buffered saline with a pH of 7.1 containing 5 g/dl human albumin + 0.46 g/dl dextrose or 1 g/dl Physiogel + 0.46 g/dl dextrose, the granulocytes were adequately preserved from the in vitro measurements.

Blood Preservation↗