PubMed Health⌕ Search

Biomedical subjects

S M Klimenko

Publications and source records attributed to S M Klimenko.

At least 19 recordsLinked to original sources
PubMed1992

Localization by immunogold labelling of HIV-1 structural proteins on Lowicryl embedded HIV-1 infected cell ultrathin sections.

Using several HIV-1-specific antibodies and the immunogold labelling technique, we have detected and localized distinct viral proteins on ultrathin sections of HIV-1 infected cells embedded in the Lowicryl K4M resin. Monoclonal antibodies (MAbs) against p24, p17 and gp160/gp41 showed a preferential labelling on viral formations still attached to the cell membrane (budding process) or free in the extracellular space. The anti-p24 and the anti-p17 MAb yielded a gold labelling not only on mature but also on immature virions where the gold particles were associated with the ring-like electron-dense material. The three HIV-1-specific antibodies against p24, p17 and p55 yielded a cross-reaction with HIV-2 in agreement with the conservation of the internal antigenic determinants of both viruses. In all instances, there was no specific immunogold labelling over the cells, suggesting that once the virus structural proteins were synthesized, they were promptly utilized for virus assembly at the plasma membrane level.

Acrylic Resins↗
PubMed1989

Detection by immunogold techniques of HIV antigens in Lowicryl ultrathin sections of infected cells.

A post-embedding technique for immunocytochemical analysis at the ultrastructural level was used to detect and localize HIV antigens on ultrathin sections of Lowicryl K4M-embedded HIV-infected cells. With serum from an AIDS patient, specific immunogold labelling was obtained exclusively on mature viral extracellular structures. The more intense reactivity was obtained with core antigens. The present immunoelectron microscopy method provides several advantages - high sensitivity of immunodetection, good preservation of cellular morphology, easy preparation procedure - which could lead to the use of this method for HIV-infected human tissues.

Acquired Immunodeficiency Syndrome↗
PubMed1986

Studies on the genetic determinants of influenza virus pathogenicity for mice with the use of reassortants between mouse-adapted and non-adapted variants of the same virus strain.

The original influenza virus strain A/USSR/90/77 (H 1 N 1) and its mouse-adapted variant, differing in their reactivity with anti-hemagglutinin monoclonal antibodies HC 22 and HC 124, were crossed in MDCK cells and in chicken embryos, and 21 clones were isolated by non-selective random cloning. In all the clones the virulence for mice was found to be linked to the antigenic specificity of hemagglutinin (HA). An independent marker, formation of filamentous forms, was reassorted with an expected frequency. In the crosses between UV-irradiated mouse-adapted variant and live non-adapted strain, with selection of clones by a mixture of monoclonal antibodies discriminating between HA of the two variants, virulence also was linked to HA gene. On the contrary, in the experiments with A/Aichi/2/68 (H 3 N 2) strain and its mouse-adapted highly virulent variant these two characteristics--virulence and HA antigenic specificity--could be dissociated. A pathogenic clone having HA of the non-adapted strain was readily obtained; its virulence, however, was weaker than that of the mouse-adapted parent. In the inter-subtypic crosses between A/USSR/90/77 and A/Aichi/2/68 the transfer of the HA gene of the mouse-adapted A/Aichi/2/68 did not confer virulence to the reassortant. The results are discussed in terms of the genetic basis of virulence acquired in the course of influenza virus adaptation to a new host.

Animals↗
PubMed1985

[Effectiveness of incorporation of spermine-condensed viral DNA into liposomes. Interaction of liposomes with cells].

DNA from bacteriophage lambda or monkey adenovirus type 7 have been condensed with spermine and included into three types of liposomes: ethereal, obtained by inverted phases technique and by Ca++ fusion. Compact DNA form presents a tor with 100 nm external diameter and 430 nm width. It can be included into 100 nm or larger liposomes. Total preparation contains 15-18% of the required liposomes. Liposomal fractions with included DNA were separated from empty liposomes by step gradient of ficoll 400. Liposomal fraction having included DNA contains 15-18% of common lipid. Liposomal interaction with monkey cell cultures has been studied.

Animals↗
PubMed1981

Investigations on the structure and morphogenesis of some arboviruses isolated in the U.S.S.R.

The morphology and some stages of morphogenesis in suckling mouse brains of hitherto serologically unclassified viruses Kaspiy (LEIV-A-63), Zavashan (LEIV-Ap-6158), Artashat (LEIV-A-2366), and Paramushir (LEIV-C-2268), isolated in the U.S.S.R., of GM-710 virus isolated in Scotland, and of Sokuluk (LEIV-K-400) virus belonging to the genus Flavivirus (family Togaviridae) were studied. Virion sizes were determined and changes in infected cells described. Based on their structure and morphogenesis, the viruses Kaspiy, Artashat, Zavashan and Paramushir were referred to the family Bunyaviridae and GM-710 virus was referred to the genus Orbivirus (family Reoviridae).

Animals↗
PubMed1981

Investigation about the surface structure of some viruses by the freeze-etching and freeze-drying methods.

An electronmicroscopic study of a suspension of Venezuelan equine encephalomyelitis (VEE) virus by freeze-drying and freeze-etching methods showed that glycoprotein peplomers are located on the surface of the lipoprotein shell. These peplomers are trimeric in shape and form a regular icosahedral surface lattice corresponding to T = 4. The modes of glycoproteins clustering for the two clones of VEE are different. Venezuelan equine encephalomyelitis (VEE) virus belongs to the alpha-viruses of the toga-virus family. Virions of VEE virus have the icosahedral nucleocapsid surrounded by a lipoprotein envelope (peplos) and contain single-stranded RNA. During budding, alpha-virus nucleocapsid acquires a host cell lipid membrane in which virus specific glycoproteins are built in (Bonsdorff et al. 1975, Harrison et al. 1974). Earlier the surface peplomer clustering was investigated only for the sindbis virus (Bonsdorff et al. 1975, Bonsdorff et al. 1978).

Enterovirus↗
PubMed1980

The surface structure of Venezuelan equine encephalomyelitis virus.

An electronmicroscopic study of a suspension of Venezuelan equine encephalomyelitis (VEE) virus by freeze-drying and freeze-etching methods showed that glycoprotein peplomers are located on the surface of the lipoprotein shell. These peplomers are organized with trimer clustering in a T = 4 icosahedral surface lattice. The mode of glycoprotein clustering for the two clones of VEE are different.

Encephalitis Virus, Venezuelan Equine↗
PubMed1980

Incorporation of influenza virus M-protein into liposomes.

M-protein from influenza virus vaccine was purified by sodium dodecyl sulfate-gel chromatography and incorporated into liposomes by solubilization with octylglucoside and subsequent dialysis. Liposomes containing M-protein formed a distinct population with a density of 1.22 g/ml on sucrose-gradient centrifugation, regardless of the net charge on the liposomes. Treatment of the liposomes by freeze-fracture followed by electron microscopic examination showed multilamellar structures in those liposomes without M-protein; liposomes containing M-protein were mulberry-like structures which appeared unilamellar. These studies show incorporation of M-protein into the lipid bilayer.

Chemical Phenomena↗
PubMed1978

Khasan virus, a new ungrouped bunyavirus isolated from Haemaphysalis longicornis ticks in the Primorie region.

Strain LEIV-776P, possessing no haemagglutinating activity was isolated in 1971 in the Primorie region (U.S.S.R.) from Haemaphysalis longicornis Neumann 1091 tick. Complement fixation (CF) tests revealed no antigenic relationship with 24 antigenic groups of arboviruses or 21 ungrouped viruses isolated from ticks. The virus isolate contains RNA and is relatively sensitive to ether and sodium deoxycholate; it is pathogenic for suckling mice and two-week-old white mice by the intracerebral route, and replicates in primary cultures of chick and duck fibroblasts and green monkey kidneys and in a continuous line of pig embryo kidney cells without any cytopathic effect. According to electron microscopy, its size is 90-110 nm. Its morphological properties permit to classify it as a member of the family Bunyaviridae.

Animals↗
PubMed1978

Comparison of influenza viruses isolated from man and from whales.

Four isolates of influenza virus strains from Moscow and Habarovsk that caused outbreaks of influenza in November and December 1977 in several cities of the USSR were studied and their haemagglutinins and neuraminidases were compared with those of other human and animal influenza viruses including A/whale/Pacific Ocean/76. In H1 tests these isolates, designated A/USSR/77, reacted with immune serum against A/FM/1/47 (H1N1) to the homologous titre, and with antiserum against A/whale/PO/19/76 virus to 1/8 of the homologous titre. In neuraminidase inhibition tests all A/USSR/77 isolates showed the presence of human N1 type neuraminidase, more closely related to A/sw/New Jersey/76 (Hsw1N1) than to A/FM/1/47 (H1N1) virus. The haemagglutinin of A/whale/Pacific Ocean/19/76 virus occupies an intermediate position between H0 and H1, but its neuraminidase is close to Nav2. The virus from whales multiplies better at low (28 degrees C) and at high (40 degrees C) temperatures than do the viruses of human origin that were tested.

Adult↗
PubMed1978

Sikhote-Alin virus, a new member of the cardiovirus group (Picornaviridae) isolated from Ixodes persulcatus ticks in Primorie Region.

A virus, designated Sikhote-Alin, was isolated in 1970 from Ixodes persulcatus ticks collected from a wild boar in the Primorie region (U.S.S.R.) Sikhote-Alin virus showed no haemagglutinating activity and no antigenic relationships with arboviruses of 12 antigenic groups, 17 ungrouped tick-borne arboviruses, porcine enteroviruses and coxsackie A (types 1-18) viruses. An one-way antigenic relationship was demonstrated by complement fixation with cardioviruses (Mengo and Columbia-SK strains). The virus contains RNA, is resistant to lipid solvents, highly thermostable in the presence of 1 M MgCl2 and its size is over 20 nm but less than 25 nm. All these properties make it possible to consider it as a new member of the cardiovirus group (genus Enterovirus; Picornaviridae).

Animal Population Groups↗
PubMed1978

Razdan virus, a new ungrouped bunyavirus isolated from Dermacentor marginatus ticks in Armenia.

A virus, designated Razdan, was isolated from Dermacentor marginatus ticks in the Armenian S.S.R. in 1973. The complement fixation tests revealed no antigenic relationships to 74 tick-borne arboviruses. The size of the virus is about 100 nm; it agglutinates goose erythrocytes at pH 5.5--7.0, is pathogenic for newborn, 14-day-old and adult white mice and multiplies in primary and continuous cell cultures. Morphological properties of the virus permit its classification as a member of the family Bunyaviridae.

Animals↗
PubMed1976

Virus "Tamdy"--a new arbovirus, isolated in the Uzbee S.S.R. and Turkmen S.S.R. from ticks Hyalomma asiaticum asiaticum Schulee et Schlottke, 1929, and Hyalomma plumbeum plumbeum Panzer, 1796.

Eleven virus strains were isolated from ticks Hyalomma asiaticum asiaticum Schulce et Schlottke, 1929, and Hyalomma plumbeum plumbeum Panzer, 1796,collected in 1971-1974 in desert regions of the Uzbee S.S.R. and the Turkmen S.S.R. According to CF test the strains were closely related to each other and not antigenically connected with viruses from antigenic groups A, B, California, CHF-Congo, Bakau, Bunyamwera, Gajam, Kaisodi, Qalyub, Kemerovo, Quaranfil, Simbu, Turlock, Hughes, Uukuniemi, Tete and 21 ungrouped viruses isolated from ticks. The virus was named "Tamdy" after the place of isolation of a prototype strain LEIV-1308 Uz. The virus does not agglutinate goose erythrocytes, it is pathogenic for suckling mice and 3 weeks old mice by intracerebral infection. Replication of a virus with CPE in cell cultures--L, Rh, A1--and without CPE--in pig embryo kidney cell cultures--was demonstrated. According to ultrafiltration and electron microscope data the size of the virus is about 90 nm. It is rather sensitive to lipid solvents and is an RNA-virus. Morphologically the virus resembles the Bunyaviridae.

Animals↗
PubMed1976

"Karshi" virus, a new flavivirus (Togaviridae) isolated from Ornithodoros papillipes (Birula, 1895) ticks in Uzbek S.S.R.

Three identical strains of an arbovirus were isolated from 475 Ornithodoros papillipes ticks collected in June, 1972, in burrows of the great gerbil (Rhombomys opimus Licht., 1882) in the environs of Beshkent, Karshinsk steppe, Uzbek S.S.R. The isolate was found to range among flaviviruses. Complement-fixation, agar diffusion precipitation and neutralization tests is tissue culture and mice indicated a one-way antigenic relationship between the isolate and West Nile virus. However, the pattern of differences between them made it possible to consider the isolated agent as a new virus, "Karshi" virus. The results of electron microscopic studies of this virus are presented.

Animals↗
PubMed1976

Persistent SV5 virus infection in continuous cell cultures.

A continuous line of guinea pig kidney cells (CGPK/H) and a continuous line of mouse fibroblasts (L/H) spontaneously infected with parainfluenza virus SV5 were found. These cultures showed no enhanced cell degeneration or symplast formation, nor was haemagglutinin accumulation or infectious virus demonstrated in them. However, regular reproduction of ribonucleoprotein (RNP) characteristic of parainfluenza viruses, morphologically complete virions and antigens producing antibody to SV5 virus were found in the cells. Focal haemadsorption neutralized by antiserum to SV5 virus was also demonstrated. The infection persisted in the cell populations for over 2 years (the observation period) under standard conditions of cell dispersion and subcultivation.

Animals↗