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Biomedical subjects

S M de Oliveira

Publications and source records attributed to S M de Oliveira.

13 recordsLinked to original sources

Double-outlet right ventricle associated with discordant atrioventricular connection and dextrocardia in a cat.

The prevalence of congenital cardiac disease in cats varies from 0.2 to 1 per cent, with males being more affected than females. This is probably an underestimate due to the fact that not all affected kittens are presented. Discordant atrioventricular connection (where the morphological right atrium connects to the morphological left ventricle, and the morphological left atrium connects to the morphological right ventricle) is an uncommon condition in humans and, to the authors' knowledge, has not to date been described in animals. The association between this anomaly and the double-outlet right ventricle (where both great arteries arise entirely or partially from the morphological right ventricle) is extremely rare in children. This report describes a case of a cat which presented with this association of cardiac defects.

Animals↗

Computer simulations for biological aging and sexual reproduction.

The sexual version of the Penna model of biological aging, simulated since 1996, is compared here with alternative forms of reproduction as well as with models not involving aging. In particular we want to check how sexual forms of life could have evolved and won over earlier asexual forms hundreds of million years ago. This computer model is based on the mutation-accumulation theory of aging, using bits-strings to represent the genome. Its population dynamics is studied by Monte Carlo methods.

Aging↗

Characterization of constitutive and putative differentially expressed mRNAs by means of expressed sequence tags, differential display reverse transcriptase-PCR and randomly amplified polymorphic DNA-PCR from the sand fly vector Lutzomyia longipalpis.

Molecular studies of insect disease vectors are of paramount importance for understanding parasite-vector relationship. Advances in this area have led to important findings regarding changes in vectors' physiology upon blood feeding and parasite infection. Mechanisms for interfering with the vectorial capacity of insects responsible for the transmission of diseases such as malaria, Chagas disease and dengue fever are being devised with the ultimate goal of developing transgenic insects. A primary necessity for this goal is information on gene expression and control in the target insect. Our group is investigating molecular aspects of the interaction between Leishmania parasites and Lutzomyia sand flies. As an initial step in our studies we have used random sequencing of cDNA clones from two expression libraries made from head/thorax and abdomen of sugar fed L. longipalpis for the identification of expressed sequence tags (EST). We applied differential display reverse transcriptase-PCR and randomly amplified polymorphic DNA-PCR to characterize differentially expressed mRNA from sugar and blood fed insects, and, in one case, from a L. (V.) braziliensis-infected L. longipalpis. We identified 37 cDNAs that have shown homology to known sequences from GeneBank. Of these, 32 cDNAs code for constitutive proteins such as zinc finger protein, glutamine synthetase, G binding protein, ubiquitin conjugating enzyme. Three are putative differentially expressed cDNAs from blood fed and Leishmania-infected midgut, a chitinase, a V-ATPase and a MAP kinase. Finally, two sequences are homologous to Drosophila melanogaster gene products recently discovered through the Drosophila genome initiative.

Animals↗

[Review of nursing literature on arterial hypertension in pregnancy].

Nursing literature review, related to pregnancy induced hypertension, from 1980 to 1999, with the objective of identifying the tendencies of those publications. 58 publications were found in the English, Portuguese and Spanish languages, being 52 articles from periodicals and 6 monographs, dissertations and thesis. Most of the articles was published in nursing periodicals (84.6%), prevailing the English ones (73.1%), written by academicians, with sole writing. The focuses nursing care (25.9%) and pharmacological treatment predominated (25.9%). An increment was observed in the publications starting from 1985. The national publications concerning the pregnancy induced hypertension are still incipient.

Female↗

Endocardial fibroelastosis in a dog.

Endocardial fibroelastosis is an uncommon congenital heart disease in dogs that may be manifested by signs of left-sided congestive heart failure. A three-month-old, male, Fila Brasileiro dog developed signs of generalised heart failure. Physical examination revealed normal temperature, ascites, and pale and cyanotic mucous membranes. The pup died just after radiography which revealed ascites, hepatomegaly, severe cardiac enlargement and pulmonary oedema. At necropsy, serosanguineous fluid in the thorax and abdomen, pulmonary oedema, right ventricular dilatation, hypertrophy and dilatation of the left ventricle, and mitral valve incompetence were observed. The histopathological examination demonstrated that the thickening of the endocardium of the left atrium and left ventricle was due to the presence of elastic and collagen fibres, although there were no signs of an inflammatory process.

Animals↗

[Blood pressure measurement in pregnancy].

This study deals with the review of the literature regarding the indirect blood pressure measurement in normal pregnant women. It shows the changes that happened with the blood pressure due to pregnancy. Polemical aspects in the procedure of blood pressure measurement are discussed; for example, which one of the Korotkoff phases (4 or 5) that better represent the diastolic blood pressure and the use of Ambulatory Blood Pressure Monitoring in pregnancy. The recommendations from different societies are emphasized (American Heart Association, British Hypertension Society, Australasian Society, National High Blood Pressure Education Program and World Health Organization).

Blood Pressure Determination↗

[Comparative study of the indirect measurement of arterial blood pressure in pregnant women with a cuff of correct width and with a standard cuff].

The hypertensive disorder of pregnancy is one of the most important complications of the pregnant cycle, presenting high risk of morbidity and mortality to the mother and the child. The marks of the systorical blood pressure and diastorical blood pressure are the parameters to diagnose the hypertensive disorder of pregnancy. Then, the precise measure of the blood pressure is an essential condition for the clinical evaluation of the pregnant woman during the antepartum course, in order to diagnose in advance, the hypertensive disorder of this period, since the raising in the blood pressure is normally the first clinical sign of the sickness (National High Blood Pressures Educational Program, 1990). The interest in the evolution of the blood pressure during the pregnancy is very old, since 1884, when Schedoff and Porockjakoff pointed out the attention to the association of the raising in the blood pressure with eclampsia (HENRY, 1936). Since then, many studies about the modification of the blood pressure caused by the pregnancy have been published, in order to recognize pathological diversions that characterize the hypertensive disorder of pregnancy. Although, the researchers are trying to identify an exact limit of the normal blood pressure during the pregnancy, there is a gap in the adaptation of the cuff to the circumference of the arm. In spite of the recommendation by the American Heart Association in 1951, which says that the width of the cuff should be 20% bigger than the diameter of the arm, no comparative study about the marks of the blood pressure during the pregnancy taken with a cuff of correct width and with a cuff of standard width, was found in the specific literature. (BORDLEY III et al., 1951) Comparing the indirect measure of the blood pressure taken with a cuff of correct width and taken with a cuff of standard width, ARCURI (1985) observed in 404 normotensive and not pregnant women between 17 and 59 years old underestimation of the blood pressure, especially in those women with thin arm. The co-relation is opposite to the circumference of the arm, what means that the smaller is the circumference the higher is the underestimation. Considering there is an underestimation of the levels of the blood pressure, when the large cuffs are used, it is called into question the jeopardy of the early diagnosis of high blood pressure of thin pregnant women. Based on the consideration presented, this study has the aim of evaluating the measurement of the blood pressure on pregnant women by using cuffs adequate to the circumference of the arm. The population of this study will be healthy pregnant women, with one alive fetus, registered in the antepartum course service of a maternity and of a community center in São Paulo city.

Adult↗

[Qualitative approach: a contribution to nursing].

This paper focus on some characteristics of the qualitative methodology. Some of these methods are explored such as: participatory research, phenomenology, grounded theory and ethnography critical theory Perspectives of their utilization in nursing research are examined.

Ethnology↗

[Ecological aspects of American tegumentary leishmaniasis: 8. Evaluation of the enzootic activity of Leishmania (Viannia) braziliensis, in forest and peridomiciliary environments of the Ribeira Valley region, São Paulo State, Brazil].

The evidence of mucocutaneous leishmaniasis transmission in the non-forest environment in the Ribeira Valley region, São Paulo, Brazil, has made this epidemiologic four years' study possible, viewing the enzootic activity of L. (V.) braziliensis. Furthermore, the natural infection in small mammals and the domestic dog population has been completed in collecting of phlebotomine sandflies in the forest and peridomiciliar environments. Positive test-results have only been found in resident dogs (Canis familiaris) with a rate of 5.6 and 2.4% for serological and parasitological test respectively. Among silvatic and synanthropic rodents collected, Oryzomys (Olygoryzomys) and Rattus rattus are pre-eminent having both been collected in equal proportions, in peridomiciliar environment. The sandfly Lutzomyia intermedia has contributed only with 166 feminine specimens due to insecticide (DDT) application on and in the human and domestic habitations. From the general epidemiologic standpoint the fragile L. (V.) braziliensis cycle in the unforested areas as well as the canine and small mammals roles as a domiciliar infection source are discussed, and their potencial in the dispersion of this parasite in the researched area is analysed too.

Animals↗

[Frequency of intestinal helminth eggs in public restrooms in Sorocaba, SP].

Four hundred and five water closets were investigated: 11 located at public squares, 2 at the railroad station, 4 at the bus station, 55 at bars and restaurants, 146 at state schools, 116 at municipal schools, 8 at private schools, 36 at public health centers, 16 at sports centers, 8 at orphanages and 3 at shopping malls. Microscopy slides with adhesive tape were used. The material was obtained by sticking the tape onto the following elements: 4 spots on the toilet seat, internal and external door knobs, latch, faucet handle and discharge valve (push button or pulling string). Out of the 405 water closets studied, 22 (5.43%) were contaminated. Eggs of Ascaris lumbricoides, Ancylostomatidae, Enterobius vermiculares, Taenia sp and Hymenolepis nana were found. In 2 water closets eggs of 2 parasites were found simultaneously.

Animals↗