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Biomedical subjects

S McIntire

Publications and source records attributed to S McIntire.

11 recordsLinked to original sources
PubMed1993

Acute noncrescentic poststreptococcal glomerulonephritis presenting with pulmonary hemorrhage.

We report a child with acute poststreptococcal noncrescentic glomerulonephritis and pulmonary hemorrhage. This patient demonstrates that: (1) poststreptococcal noncrescentic glomerulonephritis in children can be associated with pulmonary hemorrhage, (2) an expeditious renal biopsy in patients with acute or rapidly progressive glomerulonephritis and pulmonary hemorrhage can establish an early diagnosis and provide timely guidance for treatment, and (3) although not proven by controlled studies, the intravenous administration of methylprednisolone in our patient may have been effective in the treatment of pulmonary hemorrhage.

Acute Disease↗
PubMed1988

Purification and properties of the major nuclease from mitochondria of Saccharomyces cerevisiae.

The vast majority of nuclease activity in yeast mitochondria is due to a single polypeptide with an apparent molecular weight of 38,000. The enzyme is located in the mitochondrial inner membrane and requires non-ionic detergents for solubilization and activity. A combination of heparin-agarose and Cibacron blue-agarose chromatography was employed to purify the nuclease to approximately 90% homogeneity. The purified enzyme shows multiple activities: 1) RNase activity on single-stranded, but not double-stranded RNA, 2) endonuclease activity on single- and double-stranded DNA, and 3) a 5'-exonuclease activity on double-stranded DNA. Digestion products with DNA contain 5'-phosphorylated termini. Antibody raised against an analogous enzyme purified from Neurospora crassa (Chow, T. Y. K., and Fraser, M. (1983) J. Biol. Chem. 258, 12010-12018) inhibits and immunoprecipitates the yeast enzyme. This antibody inhibits 90-95% of all nuclease activity present in solubilized mitochondria, indicating that the purified nuclease accounts for the bulk of mitochondrial nucleolytic activity. Analysis of a mutant strain in which the gene for the nuclease has been disrupted supports this conclusion and shows that all detectable DNase activity and most nonspecific RNase activity in the mitochondria is due to this single enzyme.

Cations, Divalent↗
PubMed1987

Garlic burns: a naturopathic remedy gone awry.

We report the case of a child who sustained partial thickness burns from a garlic-petroleum jelly plaster, which had been applied at the direction of a naturopathic physician. A review of the literature reveals that "garlic burns" have not previously been reported, although medicinal properties of garlic have been investigated by physicians and biochemists. The pediatrician caring for children in an area where naturopathic medicine is routinely practiced should be aware of the potential side effects of plasters, poultices, and other "natural" remedies in children.

Burns, Chemical↗
PubMed1985

Epinephrine accumulation in rat brain after chronic administration of pargyline and LY 51641--comparison with other brain amines.

Concentrations of biogenic amines and metabolites were measured in regions of rat brain following administration of monoamine oxidase (MAO) inhibitors for 21 days. Epinephrine concentrations were increased from 350 to 500% following chronic administration of LY 51641, a selective inhibitor of MAO type A. Norepinephrine, dopamine and serotonin showed much less relative accumulation. The marked relative accumulation of epinephrine may be related to the efficacy of inhibitors of MAO type A in the treatment of depression.

3,4-Dihydroxyphenylacetic Acid↗
PubMed1980

Transfer-deficient cointegrates of Flac and lambda prophage.

Twelve transfer-deficient mutants of the plasmid Flac were obtained by insertion of prophage lambda into secondary attachment sites within the transfer region. Insertions into eight different tra genes were identified. These mutations were strongly polar on expression of tra genes previously mapped "downstream", and thus confirmed that the genes traA through traD form a single operon. However, some continued expression of traI suggested that this was transcribed in part from a promoter located between traD and traI, and in part from the transfer operon promoter. One insertion early in the transfer operon produced a plasmid-specific tra mutation not complemented by R100-1 or R1-19: this insertion was into a new gene (traY), located before traA as the first member of the transfer operon. Partial tra deletion mutants were obtained as 42 degrees C--survivors from several of the Flac tra::ED lambda 4 plasmids, and their properties are described.

Bacteriophage lambda↗
PubMed1979

The exploitation of lambda tra transducing phages in the study of bacterial conjugation.

A set of lambda tra transducing phages have been isolated from an abnormal lysogen in which the lambda prophage was inserted into the traB gene of Flac, and characterised both genetically and physicochemically. The phages were used in several ways to facilitate studies of bacterial conjugation: (a) they aided the identification and mapping of four new transfer genes, traU, traN, traV and traW: (b) they gave information about the location and orientation of the promoters for traM, traJ and traI: (c) a more precise physical map of the transfer region was developed from the lengths of the tra segments that they carried, and (d) the molecular weights of the traM and traJ proteins synthesised by a lambda ptraMJALEK phage were determined.

Bacteriophage lambda↗
PubMed1978

Plasmid cointegrates of Flac and lambda prophage.

Fifteen cointegrates of the plasmid Flac and prophage lambda that had suffered no detectable change in plasmid phenotype were isolated and characterized. The locations of the prophage insertions were determined by genetic analysis of deletion mutants obtained from each cointegrate as survivors of growth at 42 degrees C. In 11 cointegrates, the prophage was inserted between traI and lac, although probably in more than one location; in 3 others, it was on one side or the other of lac; and in 1 it was between lac and pif. Deletions covering all or part of the transfer region, as well as of lac and of pif, were obtained in the course of this analysis. Deletion mutants that had lost all known transfer genes were also oriT, but they retained the capacity to recircularize after transfer. Attempts were made to isolate lambda transducing phages for nearby plasmid genes from the cointegrates, and lambdaptraGD, lambdaptraD, lambdaptraI, and lambdadtraDI phages were obtained.

Coliphages↗
PubMedDate not supplied

Bar codes and the clinical laboratory: adaptation perspectives.

This paper provides an overview of the functional capabilities of bar-code technology within the laboratory environment and assesses the problems and solutions encountered during its introduction in our laboratory. An idea of the potential costs and personnel savings is offered, demonstrating that we have saved an entire full-time equivalent (FTE) medical technologist in clinical chemistry with an associated improvement in the accuracy of patient identification and a marked improvement in turnaround time for both chemistry and hematology routine procedures. We have estimated the savings of two FTE medical technologists while improving service and accuracy. Hardware costs should be recovered in 2 years.

California↗