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Biomedical subjects

S Mebel

Publications and source records attributed to S Mebel.

At least 19 recordsLinked to original sources
PubMed1985

Changes within the genus Bordetella influenced by phages.

Bordetella phages give rise to changes in the important phenotypic properties of all three Bordetella species. The changes depend on the phage system used. The treatment of Bordetella parapertussis with phages from B. pertussis led to toxic clones with manifold combinations of agglutinogens specific for all three Bordetella species. Under the influence of B. pertussis phages clones are formed which differ phenotypically from the recipient strain. Some of these clones are sensitive to some phages. In addition, the converted strains were able to propagate in the brain tissues of mice and gave rise to paralytic symptoms or death. Adhesive and hemagglutinating activities were detected in the converted strains and biochemical properties of the converted strains changed in comparison to the recipient strain. The sensitivity of converted strains to antibiotics was investigated and the tolerance of converted strains to erythromycin was used as a marker of conversion. As to their most striking features the converted strains resembled wild strains of B. bronchiseptica isolated from rabbits. While it was not possible to show the ability of pertussis phages to propagate in strains of homologous species by the agar layer method, pertussis phages did adsorb maximally on strains of B. pertussis.

Animals↗
PubMed1983

[Modern bacterial vaccines].

A well founded vaccination strategy is of crucial importance for controlling communicable diseases. The WHO Enlarged Vaccination Programme of 1976 provides to protect by vaccination all children in the world against six infection diseases - diphtheria, pertussis, tetanus, measles, poliomyelitis, and tuberculosis, a sure immunization prophylaxis being possible against them. The perspectives of further development of some classical vaccines as against cholera, typhoid fever, and pertussis are reviewed. Some recent bacterial vaccines are discussed being of special significance for controlling nosocomial infections and for protecting patients in intensive therapy and/or persons with lowered immunological defence. New ways for production of vaccine matters are shown such as capsule polysaccharides, membrane proteins, subcellular fractions, and vaccines with synthetic carrier molecules. Fundamental problems concerning the initiation of vaccination and the demands for an enlarged proof of innocence of vaccines are discussed.

Aged↗
PubMed1982

[Conversion of Bordetella parapertussis serovar through lysogeny produced by pertussis phages].

Bacteriophages from Bordetella pertussis were titrated on the indicator strain B. parapertussis 17903 by using standard soft agar techniques. Secondary growth, occasionally observed in some phage plaques, was isolated and transferred onto selective media. Judging from growth on these special media and microscopic examination the isolated clones consisted entirely of Bordetellae. Determination of the agglutinogen pattern of 160 of these clones revealed that 88% contained agglutinogen 1; 87.5% agglutinogen 14, and 80.1% agglutinogen 12 (Table 3). However, none of the strains expressed the agglutinogen pattern of either the phage donor or the recipient strain. The isolated clones were lysogenic as demonstrated by phage production and immunity against superinfection (95% of the clones).--Absorption of the monospecific antisera with whole cells from lysogenic strains resulted in a drastic decrease or even complete loss of specific antibodies towards those antigens identified by slide agglutination reactions (Table 4). Cross absorption experiments with antisera against B. pertussis, B. parapertussis and strain 73 1/7 and various strains of the genus Bordetella and with a number of lysogenic strains showing various agglutination patterns allowed the conclusion that the latter ones were serologically related to B. pertussis. The lysogenic strains completely absorbed antibodies against B. bronchiseptica, and those strains that carried the agglutinogen 14 also absorbed antibodies against, B. parapertussis (Table 5). In conclusion, these results support the necessity to revise the subdivision of the genus Bordetella into three species. A change of B. parapertussis to B. pertussis within the epidemiological processes is considered.

Agglutination Tests↗
PubMed1979

Experiences with pertussis vaccination in GDR.

Because of a high pertussis morbidity, compulsory vaccination with DPT-vaccine was started in GDR in 1964. The performing of vaccination for many years has led to a 60--100 fold decrease of morbidity. The well established system of medical care for mothers and babies and the high percent of children who spend their day in nurseries and kindergardens enables satisfying control of the vaccinees. The observed side reactions give no reason to abandon the DPT-Vaccination.

Central Nervous System Diseases↗
PubMed1977

Morphology of Salmonella typhi in different inactivation techniques.

Heat-phenol treatment of Salmonella typhi results in a denaturation of the cytoplasm and a smoothing of the cell wall with numerous ruptures. Following acetone inactivation the cell wall changes similarly, the cytoplasm is shrunken and its structures remain intact. Destruction is more intensive following heat-phenol treatment than after acetone precipitation. In both cases three contrasting layers of the cell wall can be seen.

Acetone↗
PubMed1977

Pertussis vaccine: reactivity in animal and man.

Clinical investigation of the reactivity of diphtheria-tetanus-pertussis (DTP) vaccines is a routine control in the GDR. This paper shows the results of the clinical control of 26 batches of DTP vaccine in comparison with animal tests (weight-gain-test, histamine-sensitizing test, dermonecrotic test). The animal tests gave no support to innocuity. The batches were tested on 427 children and passed the requirements for reactivity. After a storage for 2 years the control of 3 different batches was repeated. Two of them showed side reactions in children in spite of the fact that the results of the weight-gain test did not change.

Animals↗