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Biomedical subjects

S Miyamura

Publications and source records attributed to S Miyamura.

At least 37 records · Page 2Linked to original sources
PubMed1984

Initial detection of [3H]clonidine binding to solubilized rat brain alpha 2-adrenergic receptors: regulation by guanine nucleotides.

Alpha 2-adrenergic receptors labeled by [3H]clonidine (alpha 2-agonist) can be solubilized from the rat brain with a zwitterionic detergent, 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate (CHAPS). CHAPS-solubilized receptors have the same characteristics of membrane-bound alpha 2-receptors in the brain, and the regulation of receptor binding by guanine nucleotides is retained in the soluble state.

Adenosine Triphosphate↗
PubMed1984

Isolation of Rickettsia tsutsugamushi antigenically different from Kato, Karp, and Gilliam strains from patients.

Rickettsia tsutsugamushi strains from three recent patients of Tsutsugamushi disease in Niigata Prefecture were isolated primarily in mice and then in L cell cultures. By this procedure, low virulent strains to mice, as well as high virulent ones, could be isolated and cultivated serially in L cell cultures, suggesting the usefulness of L cells for isolation of this species of rickettsia. Each newly isolated strain was identified as a member of R. tsutsugamushi from the results of cross immunological tests and morphological observation. On the other hand, it was recognized that one of these rickettsiae showed immunological properties distinguishable from the prototype strains of Kato, Karp, and Gilliam by the cross complement fixation test, and also had low virulence in mice.

Animals↗
PubMed1984

[Fundamental study on tissue distribution of ceftriaxone in the field of obstetrics and gynecology].

Fundamental study on tissue distribution of ceftriaxone (Ro 13-9904, CTRX), a new cephalosporin parenteral antibiotic, was studied and the following results were obtained. CTRX had been administered by intravenous drip infusion with 1 g to 9 cases who received simple total hysterectomy. The level of CTRX in the cubital venous serum and uterine arterial serum was determined as well as the tissue concentration in the oviduct, ovary, endometrium, myometrium, cervix uteri and portio vaginalis. The level in the oviduct and portio vaginalis was generally high, although it was observed only in a few cases. The variation of the level in the myometrium was large. CTRX is expected to be clinically useful considering the fact that its half-life time is 8.5 hours, which is longer than that of existing cephalosporin antibiotics.

Adult↗
PubMed1983

Microbial transformation of maridomycin III by Serratia marcescens.

Two transformation products of maridomycin (MDM) III, MDM-S1 and MDM-S2 named after Serratia marcescens, were isolated by silica gel chromatography. NMR and IR analysis revealed that MDM-S1 and -S2 had no aldehyde group at C-18 on the macrolactone ring, and that the hydroxyl group at C-9 seemed to disappear. Although MDM-S1 and -S2 are less active against Gram-positive bacteria than starting MDM III they are interesting materials in view of the introduction of nitrogen into each molecule, and that the transformation products are produced by Gram-negative bacteria which are thought to be insensitive to macrolide antibiotics.

Bacteria↗
PubMed1983

M-9337, a new antistreptolysin, produced by Streptomyces sp.

A new biologically active substance, M-9337, was obtained from Streptomyces strain M-9337, a soil isolate. The producing organism was subsequently determined to be a new strain and named Streptomyces antihaemolyticus M-9337. The active substance was prepared as white yellow powder from culture broth by solvent extraction and silica gel thin-layer chromatography. It showed no antimicrobial activity and potent inhibitory activity against streptolysin, a type of hemolysin.

Antistreptolysin↗
PubMed1982

Congenital microangiopathic hemolytic anemia: report of a Japanese girl.

A 4-year-old Japanese girl had hemolytic anemia, thrombocytopenia, distorted and fragmented red cells in the peripheral blood, and megakaryocytosis in the bone marrow from the newborn period. These features were temporarily relieved and prevented by the infusion of fresh frozen plasma from healthy donors. This would indicate a deficiency in the patient's plasma of factor(s) that inhibit platelet aggregation in the blood vessels. An attempt to demonstrate possible in vitro deficiency of the platelet aggregation-prohibiting factor in the patient's plasma was unsuccessful. Laboratory examinations ruled out red cell fragility, abnormalities of red cell enzymes, abnormal hemoglobins, or immune reaction as underlying mechanisms. Our patient, together with three other patients in the literature with similar clinical features, may constitute a clinical entity that could be called "congenital microangiopathic hemolytic anemia".

Anemia, Hemolytic, Congenital↗
PubMed1980

Percutaneous transhepatic drainage: experience in 100 cases.

A two-step procedure for percutaneous transhepatic drainage (PTD) of the biliary tract was attempted on 101 patients with obstructive jaundice, 29 with benign and 71 with malignant lesions, and was successful in 100. With this procedure, marked clinical improvement, with reduction in levels of serum bilirubin, SGOT, SGPT and alkaline phosphatase, was achieved after 1-2 weeks as a preoperative step or for continuous drainage in inoperable cases. In one patient 8 dys after PTD, however, a complicating large intrahepatic hematoma proved fatal. Emergency operation was necessary in three of the four patients who developed bile peritonitis due to dislocation of the catheter and in one with intra-abdominal bleeding. Our results suggest that this procedure is very useful in the diagnosis and management of certain obstructive diseases of the biliary tract.

Adult↗