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Biomedical subjects

S Nagda

Publications and source records attributed to S Nagda.

8 recordsLinked to original sources

Combination of multiple microsatellite data sets to investigate genetic diversity and admixture of domestic cattle.

Microsatellite markers are commonly used for population genetic analyses of livestock. However, up to now, combinations of microsatellite data sets or comparison of population genetic parameters from different studies and breeds has proven difficult. Often different genotyping methods have been employed, preventing standardization of microsatellite allele calling. In other cases different sets of markers have been genotyped, providing differing estimates of population genetic parameters. Here, we address these issues and illustrate a general two-step regression approach in cattle using three different sets of microsatellite data, to combine population genetics estimates of diversity and admixture. This regression-based method is independent of the loci genotyped but requires common breeds in the data sets. We show that combining microsatellite data sets can provide new insights on the origin and geographical distribution of genetic diversity and admixture in cattle, which will facilitate global management of this livestock species.

Animals↗

A biomechanical analysis of the modified Tsuge suture technique for repair of flexor tendon lacerations.

Thirty-six flexor tendons from fresh frozen cadavers were randomized to three types of repairs: a Kessler-Tajima, a 4-strand modified Tsuge, and a 6-strand modified Tsuge. All repairs were accompanied by a standard epitendinous suture. The repaired tendons were then tested to initial gap and ultimate failure in an Instron machine. The average forces to ultimate failure were 31.8 N (SD, 8.8), 48.4 N (SD, 10.7), and 64.2 N (SD, 11.0) respectively. The 6-strand modified Tsuge suture was significantly stronger than the other repairs and the 4-strand modified Tsuge was significantly stronger than the 2-strand Kessler-Tajima. The 6-strand and 4-strand modified Tsuge repairs appear strong enough to withstand the forces generated during early active range of motion flexor tendon rehabilitation protocols. Clinical trials are required to evaluate the usefulness of these repairs.

Biomechanical Phenomena↗

Infant palatal obturator.

The occurrence of a congenital cleft palate greatly impedes the habituation of efficient deglutition of an infant, causing aspiration of fluids in the air passage which may lead to complications like bronchopneumonia and infection of the airway and lungs. Inadequate nourishment due to difficulty in feeding affects the health and acts as a stumbling block in the milestones of normal development. Severe nutritional deficiencies further complicate surgical closure at the right time. A palatal obturator given to the infant effectively separates the oral cavity from the nasal cavity and is of great help in feeding till the defect can be surgically repaired.

Bronchopneumonia↗

The impact of a guideline-driven computer charting system on the emergency care of patients with acute low back pain.

Federal guidelines for the treatment of acute low back pain were locally modified and made more specific. These guidelines were then programmed into a rule-based computer charting system which provides real-time advice regarding documentation, testing, treatment, and disposition of emergency department patients with this condition. In a time-series off-on experiment the system was shown to significantly improve documentation of the medical record and discharge instructions. There was little effect on the appropriateness of testing and treatment and the cost of care. These findings contrast with our previous experiment using a similar program for the care of health care workers exposed to body fluids. In that study both the appropriateness of care and the cost-effectiveness of care were substantially improved.

Acute Disease↗

EDECS: the Emergency Department Expert Charting System.

EDECS, the Emergency Department Expert Charting System, integrates clinical guidelines into the everyday practice of medicine. By generating the medical record and patient aftercare instructions, it facilitates patient care. For this reason, doctors are willing to use it. While using it, the doctors are continually presented with advice regarding documentation, testing, and treatment. Unlike guidelines that attempt to modify behavior through traditional educational methods, these computerized guidelines are seen by the physician every time she sees a patient. We have demonstrated this by directly integrating the guidelines into the process of patient care; we can increase compliance with the guidelines [1]. At present EDECS exists for the chief complaints of occupational exposure to body fluids, acute low back pain, recurrent seizure, fever in children, and males with penile discharge or dysuria. Upon examining the patient, the physician proceeds to the computer, which prompts him for essential information regarding the history and physical examination. Certain items are required for all patients with the chief complaint, others are required based on the answers to these items. Data is analyzed by the computer, which provides advice regarding testing and treatment. Once testing is completed, the system suggests a probable diagnosis and aids in patient disposition and discharge planning. Finally, EDECS prints the medical record as well as patient-specific aftercare instructions. EDECS is a user friendly system; most data is entered via mouse. It is written in the OS-based expert system shell AM(TM) and can be run on an IBM compatible PC or PC network. Rules are generally written in an "if...then" format, but more sophisticated rule structures, including Bayesian models, are used when needed. Each module contains separate subroutines for the history, physical, laboratory ordering, treatment, and disposition. These modules call each other in a dynamic fashion. The system is currently being evaluated for its effect on documentation, appropriateness of use of ancillary tests, appropriateness of use of treatments, physician satisfaction, patient satisfaction, and patient outcomes. Initial results of the system's effect on documentation and use of ancillary tests and treatments show much promise [1]. The Occupational Exposure to Body Fluids module has shown a statistically significant, and sometimes rather dramatic, increase in the level of documentation for nearly all items. Further, the advice given by EDECS has caused an increase in the appropriate use of testing and treatments. For example, unnecessary ancillary tests dropped from 1.5 per patient without the computer to 0.1 per patient with the computer's aid. EDECS facilitates quality management activities and research since it collects standardized information and stores it in an easily retrievable database format. It can also be used to educate medical students and residents about the proper care of patients with a given chief complaint. At this session, EDECS will be demonstrated, and issues regarding the development of guidelines, the encoding of guidelines in rules, and the organizational structure of the software will be presented and discussed.

Child↗

Comparison of pathology in susceptible A/J and resistant C57BL/6J mice after infection with different sub-strains of Plasmodium chabaudi.

Susceptible A/J and more resistant C57BL/6J mice were infected with Plasmodium chabaudi chabaudi 54X, P.c. chabaudi AS and Plasmodium chabaudi adami 408XZ. As expected, most C57BL/6J mice survived the infections with the different isolates. But in contrast to previous observations, not all A/J mice succumbed to infection: just over 50% of A/J mice survived infections with P.c. chabaudi 54X, while 80% survived P.c. chabaudi AS. The more virulent parasite, P.c. adami 408XZ, was able to kill all A/J mice and 20% of C57BL/6J mice after an intravenous infection with 10(5) pRBC. A detailed study of four parameters of pathology (body weight, body temperature, blood glucose and RBC counts) in both mouse strains after a P.c. adami 408XZ infection showed similar patterns to those previously reported after infection with P.c. chabaudi AS. These data suggest that environmental factors as well as parasite polymorphisms might influence the severity of malaria between susceptible and resistant mice.

Animals↗

Implementation of clinical guidelines via a computer charting system: effect on the care of febrile children less than three years of age.

OBJECTIVE: The authors have shown that clinical guidelines embedded in an electronic medical record improved the quality, while lowering the cost, of care for health care workers who incurred occupational exposures to body fluid. They seek to determine whether this system has similar effects on the emergency department care of young children with febrile illness. DESIGN: Off-on-off, interrupted time series with intent-to-treat analysis. SETTING: University hospital emergency department. SUBJECTS: 830 febrile children less than 3 years of age and the physicians who treated them. INTERVENTIONS: Implementation of an electronic medical record that provides real-time advice regarding the content of the history and physical examination and recommendations regarding laboratory testing, treatment, diagnosis, and disposition. MEASUREMENTS: Documentation of essential items in the medical record and after-care instructions; compliance with guidelines regarding testing, treatment, and diagnosis; charges. RESULTS: The computer was used in 64 percent of eligible cases. Mean percentage documentation of 21 essential history and physical examination items increased from 80 percent during the baseline period to 92 percent in the intervention phase (13 percent increase; 95 percent CI, 10-15 percent). Mean percentage documentation of ten items in the after-care instructions increased from 48 percent at baseline to 81 percent during the intervention phase (33 percent increase; 95 percent confidence interval, 28-38 percent). All documentation decreased to baseline when the computer system was removed. There were no demonstrable improvements in appropriateness of care, nor was there evidence that appropriateness worsened. Mean charges were not changed by the intervention. CONCLUSION: The intervention markedly improved documentation, had little effect on the appropriateness of the process of care, and had no effect on charges. Results for the febrile child module differ from those for the module for occupational blood and body fluid exposure (a more focused and straightforward medical condition), underscoring the need for implementation methods to be tailored to specific clinical complaints.

Child, Preschool↗