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Biomedical subjects

S Nii

Publications and source records attributed to S Nii.

At least 73 records · Page 4Linked to original sources
PubMed1983

Electron microscopic study on vaccinia virus release.

FL cells infected with the IHD-W strain of vaccinia virus were studied by scanning and transmission electron microscopy. A large number of naked virus particles were found to accumulate beneath the host cell plasma membrane and to protrude from the cell surface. It was seen in some cases that naked viral particles were released by budding not only from the cell surface but also from the surface of cytoplasmic packets which were seen along the cell periphery.

Amnion↗
PubMed1983

Co-existence of malignant squamous cells and herpes simplex virus type 2-infected cells.

A case is presented in which malignant squamous cells and herpes virus infected cells were recognized concomitantly at routine cytologic examination for detection of cervical cancer. Further examinations on admission revealed Stage Ib carcinoma of the cervix and characteristic pathologic changes of herpetic infection in the tumor cell nests. Virus was isolated from the cancerous tissues in the ectocervix following radical hysterectomy, and was identified as herpes simplex virus type 2 (HSV-2). The typical HSV-2 particles were detected by electron microscopic observation on the infected FL monolayer cells with isolation. Neutralizing antibody levels against HSV-2 declined and remained low after hysterectomy. The HSV-2, isolated and identified, might be considered as "passenger" virus. The role of this isolated virus as an etiologic agent of the cervical cancer was not clear.

Carcinoma, Squamous Cell↗
PubMed1983

Giant cell-forming variants of the Miyama strain of type 1 herpes simplex virus which differ in fusion activity.

During serial passages of a non-giant cell-forming variant (-GCr) of the Miyama strain of type 1 herpes simplex virus, a new giant cell-forming variant named +GC(81) was isolated. CPE induced by this isolate was compared with that by -GCr and also by +GC(LPV), a derivative strain of the +GC variant of the Miyama strain. Rounding of single cells was observed after infection with -GCr. Remarkable syncytial formation was induced by +GC(LPV), the syncytia containing hundreds of nuclei, while small giant cells were formed by +GC(81). The reason for the appearance of +GC variants that differ in fusion capacity is discussed.

Cell Fusion↗
PubMed1983

Two sublines of a lymphoblastoid cell line associated with Marek's disease virus.

From MDCC-MSB1, the original Marek's disease virus-associated lymphoblastoid cell line, two sublines named MDCC-MSB1-33C and MDCC-MSB1-41C were differentiated. The MDCC-MSB1-33C cell line grew well at 33 degrees, but grew poorly and finally stopped growing at 41 degrees. On the other hand, the MDCC-MSB1-41C cell line grew actively at 41 degrees, but at 33 degrees, continuous cultivation was difficult to achieve. MDCC-MSB1-33C grew suspended in the liquid medium, as did the original producer MDCC-MSB1 cell line, while MDCC-MSB1-41C grew attached to the substrate. Moreover, in soft agar, MDCC-MSB1-33C formed loose colonies, but MDCC-MSB1-41C formed packed colonies. Neither Marek's disease viral antigens nor herpes-type particles could be detected in either subline, although a Marek's disease tumor-associated surface antigen was found on the surface of both.

Animals↗
PubMed1981

Consistent appearance of microtubules in cells productively infected with various strains of type 2 herpes simplex virus.

Electron microscopic examination showed microtubular structures in FL cells infected with all 18 strains of HSV-2 examined, but not in cells infected with 9 strains of HSV-1. These structures were also detected in other cultured cells (Vero and Earle's L cells) infected with HSV-2, and also in vivo in cells, such as neuronal cells of the spinal ganglia and liver cells, of one-day-old suckling mice (DDD strain) infected with this type of virus. Thus the microtubules were consistently detected in cells productively infected with HSV-2. No other herpesviruses so far examined produced microtubular structures such as those observed in HSV-2 infected cells.

Animals↗
PubMed1980

Experimental studies on genital herpetic infection in mice.

Female ICR mice were infected with HSV-1 and HSV-2 by inserting a cotton pellet soaked in viral solution (10(7-8) PFU/m1) into the vagina. The appearance of giant cells and formation of intranuclear inclusions were detected in the epithelial layer of the uterus 24 h after intravaginal inoculation. These histopathological changes were pronounced 3 to 4 days after virus inoculation and then gradually disappeared in the next few days. Results of fluorescent antibody studies on the appearance of viral antigens in infected uterine tissues and results of viral infectivity titrations of emulsified samples of infected uteri coincided well with the histopathological observations on the general course of virus infection. The degree of histopathological involvement caused by HSV-1 was somewhat less than that caused by HSV-2, and the laboratory strains of HSV-1 so far examined (HF and Miyama) were found to be especially weakly pathogenic.

Animals↗
PubMed1977

Ultrastructural study of cell cultures infected with swinepox and orf viruses.

Monolayer cells of a porcine kidney cell line were infected with the PP-1 strain of swinepox virus, while secondary or third subcultured monolayer cells of African green monkey kidney were inoculated with the Iwate BT-9 strain of orf virus. Those infected cells were fixed when CPE became remarkable in the monolayers and examined by electron microscope. In the cytoplasm of cells infected with both viruses, various immature forms of viral particles in different developmental stages were observed. Micells were detected very close to the opening of immature particles. Mature particles asssociated with a double membrane were frequently observed. From those observations it was suggested that the developmental sequence of both viruses is essentially the same as that of vaccinia (Dales, 1973). Besides various virus forms as well as factories, the following ultrastructural changes were noted in swinepox infected cells, i.e. 1) intranuclear inclusions which consist of very fine filaments, 2) fibrillar structures with cross striations located in the nuclear inclusions, and 3) similar striated fibrillar structures in or just adjacent to virus factories (B type inclusions) in the cytoplasm. Those observations made with in vitro cells are in good accordance with the descriptions by previous investigators on in vivo materials. Accordingly those ultrastructural changes characterize the swinepox infection.

Animals↗
PubMed1976

Electron microscopic observations of FL cells infected with herpes simplex virus. III. Dense bodies.

FL cells infected with the -GCr Miyama strain of herpes simplex virus at an adsorbed multiplicity of approximately 10 were fixed at late stages of infection and examined by electron microscopy. Dense bodies containing electron-dense material and surrounded by a limiting membrane were occasionally observed in the perinuclear disternae and in intranuclear vacuoles. Budding of electron-dense material to the cisternae with acquisition of a limiting membrane at the inner nuclear membrane was also occasionally observed. These findings are in constrast with observations that in cells infected with cytomegalovirus numerous dense bodies and their budding process were observed only in the cytoplasmic area.

Amnion↗