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S Nowicki

Publications and source records attributed to S Nowicki.

At least 73 records · Page 4Linked to original sources
PubMed1994

Decay-accelerating factor is expressed in the human endometrium and may serve as the attachment ligand for Dr pili of Escherichia coli.

PROBLEM: We evaluated the hypothesis that different tissue substructures in uteri may express decay accelerating factor (DAF), a complement regulatory protein that also may serve as ligand for bacterial attachment. METHOD: Purified Dr pili, anti-Dr pili IgG, anti-DAF (SCR-3) IgG, and fluorescein-isothiocyanate-conjugated secondary IgG were used for binding and inhibition experiments. RESULT: We observed staining of endometrial glands, spiral arterioles, and myometrial arteries with Dr adhesin (pili) and anti-DAF (SCR-3) IgG, and found variation in distribution and amount of Dr ligands in different individuals. Anti-DAF (SCR-3) IgG blocked the binding of Dr pili to the endometrium. CONCLUSION: Presence of DAF in endometrium may protect tissues from complement-induced damage. Differences between individuals in DAF density in the endometrium may affect sensitivity to attachment of Dr-bearing E. coli and/or complement activation.

Adhesins, Bacterial↗
PubMed1993

Short consensus repeat-3 domain of recombinant decay-accelerating factor is recognized by Escherichia coli recombinant Dr adhesin in a model of a cell-cell interaction.

A bacterial pathogen that is important in both urinary tract and intestinal infections is Escherichia coli which expresses Dr or related adhesins. In this report, we present a model for testing cell-cell interaction, using both molecularly characterized laboratory cells that express recombinant molecules of human decay-accelerating factor (DAF), and recombinant bacterial Dr colonization factors. Dr adhesin ligand was identified as DAF (CD55), a membrane protein that protects autologous tissues from damage due to the complement system. Structure-function studies mapped the adhesin-binding site on the DAF molecule. A single-point substitution in the third short consensus repeat domain, Ser165 to Leu, corresponding to the Dra to Drb allelic polymorphism, caused complete abolition of adhesin binding to DAF.

Adhesins, Escherichia coli↗
PubMed1993

Kinematics of birdsong: functional correlation of cranial movements and acoustic features in sparrows.

The movements of the head and beak of songbirds may play a functional role in vocal production by influencing the acoustic properties of songs. We investigated this possibility by synchronously measuring the acoustic frequency and amplitude and the kinematics (beak gape and head angle) of singing behavior in the white-throated sparrow (Zonotrichia albicollis) and the swamp sparrow (Melospiza georgiana). These birds are closely related emberizine sparrows, but their songs differ radically in frequency and amplitude structure. We found that the acoustic frequencies of notes in a song have a consistent, positive correlation with beak gape in both species. Beak gape increased significantly with increasing frequency during the first two notes in Z. albicollis song, with a mean frequency for note 1 of 3 kHz corresponding to a gape of 0.4 cm (a 15 degrees gape angle) and a mean frequency for note 2 of 4 kHz corresponding to a gape of 0.7 cm (a 30 degrees gape angle). The relationship between gape and frequency for the upswept third note in Z. albicollis also was significant. In M. georgiana, low frequencies of 3 kHz corresponding to beak gapes of 0.2-0.3 cm (a 10-15 degrees break angle), whereas frequencies of 7-8 kHz were associated with flaring of the beak to over 1 cm (a beak angle greater than 50 degrees). Beak gape and song amplitude are poorly correlated in both species. We conclude that cranial kinematics, particularly beak movements, influence the resonance properties of the vocal tract by varying its physical dimensions and thus play an active role in the production of birdsong.

Animals↗
PubMed1993

Involvement of renal dopamine synthesis in the diuretic effect of furosemide in normohydrated rats.

The present study was designed to investigate whether the modification of dopamine synthesis affects furosemide responses. Experiments were performed on pentobarbital-anesthetized rats. Basal urine flow was approximately 3 microliters/min-1/g-1 of kidney weight (k.w.); furosemide (0.2 mg/kg-1 i.v.) induced a rapid diuretic effect (19.3 +/- 1.4 microliters/min-1/g-1 of k.w.). The dopadecarboxilase inhibitor, benserazide (25 mg/kg-1 i.v.), reduced furosemide-induced diuresis to 8.3 +/- 2.1 microliters/min-1/g-1 of k.w., whereas levo-dihydroxyphenylalanine (L-dopa; 1 micrograms/kg-1/min-1 infused for 60 min) increased it to 41.4 +/- 6.1 microliters/min-1/g-1 of k.w. Natriuretic response and fractional Na+ excretion induced by furosemide were significantly lower in benserazide-treated and higher in L-dopa-treated animals. Urine dopamine (DA) excretion was enhanced by furosemide from 0.44 +/- 0.05 to 0.98 +/- 0.22 ng/min-1/g-1 of k.w. and was markedly reduced in benserazide-pretreated animals, whereas both basal DA excretion and that induced by furosemide were increased significantly during L-dopa infusion. However, in benserazide- or L-dopa-treated animals, basal urine flow was not different from the control group. Urine furosemide excretion was reduced by 60% by benserazide treatment and increased by 62% during L-dopa infusion. The results are consistent with the suggestion that although endogenous DA is apparently unimportant in the maintenance of basal urine output, it is involved in furosemide-induced diuresis. The diuretic response can be altered by acute administration of substances that affect dopamine synthesis.

Animals↗
PubMed1991

Role of dopamine1-receptors in mediating renal responses to furosemide in the rat.

1. Dopamine (DA) infusion enhanced the diuresis, natriuresis and kaliuresis evoked by furosemide, but this increase was significantly lower in the presence of SCH 23390, a selective DA1-dopaminergic antagonist. 2. Water, Na+ and K+ excretion induced by furosemide were reduced by haloperidol, and SCH 23390, whereas they were not affected by +/- sulpiride, a preferentially DA2-dopaminergic antagonist. 3. The treatments used did not modify the mean blood pressure during the experiments. 4. Our data show that renal responses to furosemide are attenuated by the DA1-dopaminergic antagonist. It is possible that endogenous DA, stimulating DA1 receptors in the rat kidney may be an important factor involved in the Na+, K+ and water excretion evoked by furosemide.

Animals↗
PubMed1991

Factors affecting the accuracy of facial affect recognition.

The effect of subjects' locus of control of reinforcement and self-monitoring orientations on their ability to identify the emotional meaning of facial expressions was evaluated under competitive and noncompetitive instructions. On the basis of social learning principles, it was predicted that internally controlled, high-self-monitoring American college students would be the most accurate; the results confirmed the prediction. The impact of locus of control and self-monitoring orientations was greatest on male subjects who had received competitive instructions. In addition, female subjects were more accurate than male subjects, and positive emotions were more often correctly identified than negative ones.

Adult↗
PubMed1990

Diuretic and natriuretic effect of a brain soluble fraction that inhibits neuronal Na+,K(+)-ATPase.

The separation by Sephadex G-50 of two subfractions, peak I and II, from the brain soluble fraction has been previously described. These fractions were able to stimulate and inhibit synaptosomal membrane Na+,K(+)-ATPase, respectively (Rodríguez de Lores Arnaiz and Antonelli de Gómez de Lima, Neurochem. Res. 11, 933-948, 1986). Experimental evidence indicates that the alteration of Na+,K(+)-ATPase activity may result in changes of renal and cardiovascular parameters. In the present study, we have analyzed the effect of peak I and II fractions prepared from rat cerebral cortex on water and sodium excretion and on heart rate and arterial pressure in normotensive anesthetized rats. It was observed that water and sodium excretion were not modified by the administration of peak I fraction but that they were increased by peak II fraction. The cardiovascular parameters were not significantly modified by either of the fractions. The results indicate that brain soluble factor (s) which is (are) present in peak II fraction may modify some aspects of renal physiology after systemic administration.

Animals↗
PubMed1989

Testosterone induction of song in photosensitive and photorefractory male sparrows.

Song in male songbirds is activated by the sex steroid testosterone (T). Using male song sparrows (Melospiza melodia), we compared effects of T in the normal spring state of photosensitivity (i.e., when the pituitary-gonadal axis is sensitive to stimulation by increasing daylength) and in the late summer-early fall state of photorefractoriness (i.e., when they are insensitive to increasing daylength). Photosensitive males experienced short days for 8 weeks and then long days for another 22 weeks to induce photorefractoriness. T implants were given to the birds twice, first when on short days and photosensitive, and second when on long days and photorefractory. Song rates were compared among 5 conditions: (1) photosensitive, short days, low T titers; (2) photosensitive, short days, high T titers; (3) photosensitive, long days, high T titers; (4) photorefractory, long days, low T titers; and (5) photorefractory, long days, high T titers. Plasma levels of T were monitored throughout the experiment by radioimmunoassay. T was equally effective in inducing song in both the photosensitive and photorefractory conditions. Thus, no seasonal change was found in the sensitivity to hormone action of the neural target sites mediating this behavior in song sparrows. Photosensitive birds sang at a higher rate when on long days than when on short days, however, even though there was no concomitant increase in plasma levels of T. This finding suggests that environmental factors can alter the expression of song activated by similar levels of T.

Animals↗
PubMed1989

The relation of hostile nonverbal communication styles to popularity in preadolescent children.

Some assumptions of interpersonal theory were tested among a population of preadolescent children. Children were videotaped while interacting with a same-sex peer, and their verbal and nonverbal communications were judged by other peers who were not participants in the study. Children rated as socially unattractive by their peers had communication styles characterized by nonverbal visual hostility. These results support the assumption that negative nonverbal communications are associated with relationship difficulties.

Child↗
PubMed1989

Discrimination of facial expressions of emotion by depressed subjects.

A frequent complaint of depressed people concerns their poor interpersonal relationships. Yet, although nonverbal cues are considered of primary importance in interpersonal communication, the major theories of depression focus little attention on nonverbal social perception. The present study investigated the ability of depressed, disturbed control, and normal American adults to make rapid discriminations of facial emotion. We predicted and found that depressed subjects were slower than normal subjects in facial emotion discrimination but were not slower in word category discrimination. These findings suggest that current theories of depression may need to address difficulties with nonverbal information processing. There were also no significant differences between depressed and disturbed control subjects, suggesting that the unique social-behavioral consequences of depression have yet to be identified.

Adolescent↗
PubMed1986

Bilateral syringeal interaction in vocal production of an oscine bird sound.

The vocal organ, or syrinx, of oscine birds has two parts, each of which has generally been presumed to operate independently of the other. A significant counter-example is now demonstrated in the production of a common vocalization by the black-capped chickadee (Parus atricapillus), in which the two acoustic sources interact in a nonlinear fashion. This bird produces a sound with multiple frequency components that are heterodyne products resulting from cross-modulation between two signals, thus providing evidence that avian phonation can involve cooperative coupling between the two syringeal sources.

Animals↗
PubMed1986

The circumplexity of Leary's Interpersonal Circle: a multidimensional scaling perspective.

To investigate the circulant structure of the 16 scales comprising the Interpersonal Checklist (ICL; Laforge & Suczek, 1955), intercorrelation matrices for mate and female undergraduate ICL scores were subjected to multidimentional scaling analysis. Results showed the following: (a) while the 16 ICL scales fall in a rough circular array, measurement gaps exist in the friendly-dominant and hostile-submissive quadrants; (b) sixteenths A-B-P and L-M-N were misarranged; (c) stress coefficients for a three-dimensional solution were in an acceptable range. Implications of these findings for future research and anchoring of the ICL to Kiesler's (1983) "1982 Interpersonal Circle" are discussed.

Journal Article↗
PubMed1986

Bilateral syringeal coupling during phonation of a songbird.

The syrinx of oscine birds ("true songbirds") is a double vocal organ, and each side has generally been presumed to function independently under separate neural control during phonation. A significant counterexample is demonstrated here in the production of a common vocalization by the black-capped chickadee (Parus atricapillus), in which the 2 acoustic sources of the syrinx interact in a nonlinear fashion. The chickadee produces a sound with multiple frequency components that superficially resemble harmonics. An analysis of vocal production after unilateral and bilateral syringeal denervation shows instead that these frequency components are sum and difference frequencies, or heterodyne frequencies, resulting from cross-modulation between the 2 syringeal sides. A limited form of this bilateral coupling may be achieved after unilateral denervation of either syringeal side but not after bilateral denervation. Unilaterally denervated birds are capable of significant improvement in coupling after 10 d, too short a period for neural regrowth. These results suggest that coupling arises from a passive physical interaction between the 2 syringeal sources which is activated or regulated in some fashion by neural control from either side.

Animals↗