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Biomedical subjects

S O Knowles

Publications and source records attributed to S O Knowles.

12 recordsLinked to original sources
PubMed2006

Dose response effects of long-acting injectable vitamin B12 plus selenium (Se) on the vitamin B12 and Se status of ewes and their lambs.

AIM: To determine the effect of increasing doses of long-acting injectable vitamin B12 plus selenium (Se) given pre-mating on the vitamin B12 and Se status of ewes and their lambs from birth to weaning. METHODS: Four groups of 24 Poll Dorset ewes each were injected 4 weeks pre-mating with different doses of a long-acting vitamin B12 + Se product, containing 3 mg vitamin B12 and 12 mg Se per ml. The treatment groups received 5 ml (15 mg vitamin B12 + 60 mg Se), 4 ml (12 mg vitamin B12 + 48 mg Se), 3 ml (9 mg vitamin B12 + 36 mg Se), or no vitamin B12 or Se (control). Twelve of the twin-bearing ewes per group were selected for the study. Efficacy of the product was evaluated from changes in the concentrations of vitamin B12 in serum and liver, and of Se in blood, liver and milk in the ewes during gestation and lactation, and in their lambs from birth to weaning. Pasture samples in paddocks grazed by the ewes and lambs were collected at about 2-monthly intervals from 200-m transects. RESULTS: The flock was Se-deficient, as the mean initial concentration of Se in the blood of ewes was 182 (SE 20.3) nmol/L. Compared with untreated controls, all doses significantly (p < 0.01) increased concentrations of Se in the blood of ewes for at least 300 days. Selenium concentrations in milk were likewise increased throughout lactation, as were those in the blood and liver of lambs. The mean concentration of vitamin B12 in the serum of ewes was initially > 1,000 pmol/L, but this decreased within 28 days to < 460 pmol/L. Treatment with the 5-ml and 4-ml doses raised serum vitamin B12 concentrations of ewes for at least 176 days (p < 0.01), while their lambs had significantly greater concentrations of vitamin B12 in serum and liver for less than 37 days after birth. Tissue concentrations and duration of elevation of both vitamin B12 and Se were proportional to the dose administered. The mean concentrations of Se and cobalt (Co) in the pastures were 32 and 74 microg/kg dry matter (DM), respectively. CONCLUSIONS: Injecting ewes from a Se-deficient flock 4 weeks prior to mating with 48 or 60 mg Se and 12 or 15 mg vitamin B12 increased and maintained the Se status of ewes for at least 300 days, and of their lambs from birth to weaning. The vitamin B12 status of ewes was increased for at least 176 days and that of their lambs for less than 37 days. Due to the proportional nature of the response to increasing dosage, the dose rate of the formulation tested can be adjusted according to the severity of Se and Co deficiency in a flock. CLINICAL SIGNIFICANCE: A single subcutaneous injection of vitamin B12 + Se administered pre-mating to Se-deficient flocks is likely to prevent Se deficiency in ewes and their lambs until weaning, as well as increase the vitamin B12 status of ewes and their lambs until 5 weeks after lambing.

Animals↗
PubMed2004

Copper oxide needles administered during early pregnancy improves the copper status of ewes and their lambs.

AIM: To determine the effect of copper oxide (CuO) needles administered orally to ewes in early pregnancy on the copper (Cu) status of ewes throughout gestation and lactation, and of their lambs from birth to weaning. METHODS: In mid-April, after mating, 12 twin-bearing ewes were given an oral capsule containing 5 g CuO needles while 12 others served as untreated controls. Changes in Cu status were monitored by determining serum and liver Cu concentrations on Days 1, 62, 117, 153, 185 and 216 in the ewes, and at 1, 36, 68 and 99 days of age for lambs. Pasture herbage samples were collected at about 60-day intervals for Cu, molybdenum (Mo), iron (Fe) and sulphur (S) determinations. RESULTS: Copper status of the ewe flock was adequate, as initial mean serum and liver Cu concentrations were 15 micromol/L and 1,060 micromol/kg fresh tissue, respectively. The CuO needles did not affect serum Cu concentrations of the ewes or their lambs. Mean serum Cu concentration of all lambs at birth was about half that of ewes (8 vs 17 micromol/L), regardless of Cu supplementation, and not until at least 68 days of age was it similar to the dams'. Liver Cu concentrations of lambs at birth were also lower than that of the ewes (380 vs 640 micromol/kg fresh tissue among the controls), but changed little over time. CuO treatment increased liver Cu concentration in ewes for at least 185 days and in lambs for 36 days (p<0.05). Among untreated ewes, there was a seasonal decline in mean liver Cu concentrations, which were highest in autumn and lowest in early spring (1,060 vs 370 micromol/kg fresh tissue). The mean pasture mineral concentrations were Cu 5.7, Mo 0.48, Fe 194 and S 2,900 mg/kg dry matter (DM). CONCLUSIONS: CuO needles administered to ewes in early pregnancy increased their Cu status through gestation and early lactation, and the Cu status of their lambs for 36 days from birth. Serum Cu concentration was not affected by treatment but a marked rise was observed in all lambs between birth and 10 weeks of age. CLINICAL RELEVANCE: Copper deficiency in young lambs may be conveniently and effectively prevented by treating ewes with CuO needles during early pregnancy. The serum Cu concentration in lambs <8 weeks old may not reflect the Cu status of the flock.

Journal Article↗
PubMed2004

Adding nutritional value to meat and milk from pasture-fed livestock.

Staple meat and milk provide excellent nutrition, but when traditional foods and ingredients are tailored to meet the particular nutritional or lifestyle demands of a population they become even more attractive and valuable. These foods can be considered as delivery systems for health-promoting nutrients. Nutritional improvement of meat and milk can be achieved several ways, preferably by making desirable changes on-farm to directly improve the food without subsequent manipulations. Scope for these changes is limited by animal homeostasis, but alternatives may be less desirable. Methods in vivo that suit typical pastoral farming practice and can complement the solving of animal health and production problems include: selection of traits or phenotypes; specialty diets; long-acting parenteral supplements; and modification of ruminal microflora. Successful techniques to increase the concentration of calcium, selenium, iodine and iron in milk or meat are described. Manipulations to change composition are only one part of bringing tailored foods to market. Commercial realisation of these new products needs the initiative and collaboration of scientists, veterinarians, growers and producers responding to market pull. The uptake of future biotechnologies to capture more value inside the farm gate will also be required if the pastoral industry in New Zealand is to sustain a global competitive advantage.

Journal Article↗
PubMed2003

Growth response to increasing doses of microencapsulated vitamin B12 and related changes in tissue vitamin B12 concentrations in cobalt-deficient lambs.

AIM: To investigate growth response of cobalt deficient lambs to increasing doses of microencapsulated vitamin B12, and to measure associated changes in serum and liver vitamin B12 concentrations over 243 days. METHODS: From a flock grazing pastures that had low cobalt (Co) levels (about 0.06 mg Co/kg dry matter), 4-6-week-old lambs (n=137) were assigned to four groups and received either no treatment or a subcutaneous injection of 3.0, 4.5 or 6.0 mg of microencapsulated vitamin B12 on Day 1. At approximately monthly intervals, all lambs were weighed and blood samples were collected from a selection (n=10) of monitor animals, up to Day 243. Liver biopsies were also carried out on the monitor lambs (n=8) on Days 1, 124 and 215. RESULTS: The vitamin B12-treated lambs grew significantly faster (p<0.001) than untreated animals. Liveweights after 243 days were 28, 45, 45 and 47 kg for the untreated, 3.0, 4.5 and 6.0 mg vitamin B12-treated lambs, respectively. Of the initial group of untreated lambs, 68% had to be removed before the end of the trial because of substantial weight loss, but none of the treated animals were similarly afflicted. Serum vitamin B12 concentrations increased in all vitamin B12-treated lambs, reaching a peak at Day 25, and those of the 4.5 and 6.0 mg vitamin B12-treated lambs remained significantly higher (except at Day 124) than the untreated lambs to Day 187. However, at Day 124, but not Day 215, the liver vitamin B12 concentrations of treated lambs were two to three times higher than those of controls. CONCLUSIONS: The growth rates of Co deficient lambs were markedly improved by injection of 3.0, 4.5 or 6.0 mg of microencapsulated vitamin B12, and liveweights were maintained for at least 243 days. Serum vitamin B12 concentrations were related to this growth response; concentrations of <220 pmol vitamin B12/l were associated with a 95% probability that lambs were Co deficient and would thus respond to Co/vitamin B12 supplementation. Based on these data, the current New Zealand reference criteria for Co deficiency should be reviewed. CLINICAL SIGNIFICANCE: An injection of 3 mg microencapsulated vitamin B12 given to lambs at tailing will treat Co deficiency and will increase and maintain liveweights in a flock for up to 8 months.

Journal Article↗
PubMed2002

A reference curve using blood selenium concentration to diagnose selenium deficiency and predict growth responses in lambs.

AIMS: To quantify the relationship between blood selenium (Se) concentration and growth response to Se supplementation in lambs, in order to assess the reliability of current reference ranges and to predict the magnitude of likely responses to supplementation. METHODS: Data from 24 Se-supplementation growth-response trials which used the following general protocol were collated and analysed: in each trial, weaned lambs (n=20-30) were allocated randomly to untreated or to Se-supplemented groups. Supplemented groups received 5 mg Se/month as Na2SeO3 in 20 trials, and 1 mg Se/kg liveweight as BaSeO4 in 4 trials. The duration of the trials was 150-196 days, during which time all lambs were weighed every 4-6 weeks and blood samples were taken at various intervals from 10 lambs/group. RESULTS: Among the 24 trials, flock mean blood Se concentrations of the untreated lambs ranged from 32-569 nmol/l, and growth rate of supplemented lambs was -3 to 63 g/day higher than that of untreated lambs. The relationship between blood Se concentration (X) and growth response (Y) was described by the following hyperbolic-decay equation: Y = -2.6 (SE 3.6) + 1850 (SE 230) / X; (R2=0.74). CONCLUSIONS: For Se-deficient lambs, the potential for a growth response to Se supplementation was strongly related to blood Se concentration. Economically significant liveweight gains of 10 g/day were observed when initial blood Se concentrations were 130 nmol/l. CLINICAL RELEVANCE: Blood Se concentrations can be used to diagnose Se deficiency in lambs and to predict the magnitude of likely growth responses to Se supplementation.

Journal Article↗
PubMed1999

Significance of amount and form of dietary selenium on blood, milk, and casein selenium concentrations in grazing cows.

Organic selenized yeast enriched with selenoamino acids or inorganic sodium selenate (Na2SeO4) was administered per os three times weekly as a drench for 133 d to previously unsupplemented cows that were grazing low Se pastures. Treatment groups received the equivalent of 2 or 4 mg of Se/d of either supplement form. Control cows did not receive a drench. Samples of blood and milk were collected regularly throughout the trial. Selenium concentrations in blood, milk, casein, and liver and glutathione peroxidase activity in blood and liver are reported as responses per milligram of Se intake. Mean blood Se concentrations in treated cows increased steadily and, by d 133, were 4.7 to 8.8 times that in controls. Selenized yeast was 2 to 3 times more effective than was Na2SeO4, and low Se intakes were 27% more efficient per milligram of Se administered than were high Se intakes at increasing milk Se concentration. Casein Se content mirrored that of milk; among all treated and control cows and throughout the trial, the molar ratio of Se in casein as a percentage of the Se in whole milk was constant at 71 +/- 1.2%. The Se concentration in liver biopsies taken on d 133 was indicative of total Se intake during the trial and ranged from 920 to 3920 nmol of Se/kg of fresh weight. These results demonstrate the differing efficacy of organic and inorganic Se dietary supplements to increase dairy cow Se status and to enhance Se content of milk and casein.

Animals↗
PubMed1998

Changes in fatty acid composition of lipids from birds, rodents, and preschool children exposed to lead.

Chronic treatment with inorganic lead (Pb) has been shown to increase the proportion of arachidonic acid (ArA), as well as the arachidonate/linoleate (ArA/LA) ratio, in the fatty acids of lipids from a variety of avian tissues. Changes in two fatty acid-mediated phenomena, peroxidation of membrane lipids and synthesis of eicosanoid cytokines, are associated with this enhanced ArA content. The authors are not aware of any reports in the literature in which these effects of Pb have been described for any animals other than birds. In the current study, the authors investigated the effect of Pb on lipid metabolism in three species: avian, rodent, and human. The group of children identified as suffering environmental Pb exposure were from a Pb-surveillance program and had blood Pb concentrations (PbB) averaging 23 microg/dL. Turkey poults fed 100 ppm dietary Pb as Pb acetate-trihydrate for 19 d had a PbB of 46 microg/dL. Gastric intubation of rats with 80 mg Pb/kg/d for 10 d resulted in a PbB of 74 microg/dL. We analyzed fatty acid composition of whole blood from children, poults, and virgin rats. Low-dose (nongrowth inhibitory) Pb exposure resulted in significantly increased ArA concentration and ArA/LA ratio in blood from all species. Also analyzed were plasma and liver of poults, virgin rats, and pregnant rats and their fetuses. In plasma and liver from Pb-treated poults and virgin rats, ArA and the ArA/LA ratio were again enhanced. Pb intoxication also affected omega3 composition, increasing the concentrations of all long-chain omega3 fatty acids of fetuses from Pb-treated pregnant dams. The authors propose that altered fatty acid metabolism may be responsible for some indications of Pb poisoning. Possible consequences mediated through lipid peroxidation and production of ArA-derivative eicosanoids are considered.

Animals↗
PubMed1996

Dietary lead alters fatty acid composition and membrane peroxidation in chick liver microsomes.

Inorganic Pb acetate is a pro-oxidant, and peroxidation damage to cellular membrane lipids, leading to membrane fragility and permeability, is a likely consequence of Pb poisoning. In addition to the systemic peroxidation that occurs in vivo, Pb-contaminated feedstuffs can contribute preformed peroxides. Treatments with dietary Pb that have been shown to increase tissue peroxide levels in animals may be related to the consumption of preformed peroxides from the diet. In the current study, we evaluated the possible separate effects of feed and systemic peroxides by administering equivalent doses of Pb acetatetrihydrate to chicks via either 1,500 ppm Pb in the diet or via gastric intubation. Peroxidation of lipids in hepatic microsomal membranes (assessed as malonyldialdehyde production) from birds intoxicated with Pb by either route of administration was more than double that of untreated controls. Also, both routes of Pb exposure doubled the concentration of hepatic microsomal arachidonic acid, a peroxidizable polyunsaturated fatty acid. In the data reported here, we show that tissue peroxide levels are unaffected by the method of oral Pb administration and thus, by inference, independent of peroxide content of the feed.

Administration, Oral↗
PubMed1993

Is lead toxicosis a reflection of altered fatty acid composition of membranes?

1. The premise of this review is that many of the biological effects of Pb are reflection of tissue peroxidation. 2. Enhanced tissue levels of arachidonic acid in Pb toxicosis appear to be involved in the peroxidative changes. 3. The altered arachidonate metabolism may be related to changes in membrane structure and function. 4. The induction of enhanced glutathione levels in animal tissues by Pb may afford protection from peroxidative damage.

Animals↗
PubMed1990

Dietary modification of lead toxicity: effects on fatty acid and eicosanoid metabolism in chicks.

1. The influence of nutrition and chronic lead (Pb) administration on the level of eicosanoids and their precursors was evaluated in serum and liver from chicks fed defined diets. 2. Dietary Pb consistently increased arachidonic acid, the arachidonate/linoleate ratio, and hepatic non-protein sulfhydryl concentration. 3. Hepatic microsomal fatty acid elongation activity was decreased by Pb. 4. Cottonseed oil, calcium and methionine interacted with Pb, affecting liver and serum fatty acid profiles. 5. Tissue levels of leukotriene C4 rose significantly with Pb or correlated with Pb-compromised body weight in only one experiment. Serum prostaglandins E2 and F2 alpha were unchanged by treatments. 6. The results suggest that an increase in precursors is not consistently reflected by tissue levels of eicosanoids.

Animals↗
PubMedDate not supplied

Lead disrupts eicosanoid metabolism, macrophage function, and disease resistance in birds.

Lead (Pb) affects elements of humoral and cell-mediated immunity, and diminishes host resistance to infectious disease. Evidence is presented supporting a hypothesis of Pb-induced immunosuppression stemming from altered fatty acid metabolism, and mediated by eicosanoids and macrophages (MO). Chronic Pb exposure increases the proportion of arachidonate (ArA) among fatty acids in lipid from avian tissues, and this change provides precursors for eicosanoids, the oxygenated derivatives of ArA that mediate MO acute inflammatory response. In the current study, we showed that the concentration of ArA in phospholipids of MO elicited from turkey poults fed 100 ppm dietary Pb acetate was twice that of controls. In vitro production of eicosanoids by these MO was substantially increased, and this effect was most pronounced following lipopolysaccharide stimulation: prostaglandin F2 alpha was increased 11-fold, thromboxane B2 increased threefold, and prostaglandin E2 increased by 1.5 times. In vitro phagocytic potential of these MO was suppressed, such that the percentage of MO engulfing sheep red blood cell (RBC) targets was reduced to half that of control MO. In vivo susceptibility of Pb-treated and control birds to Gram-negative bacteria challenge was also evaluated. The morbidity of chicks inoculated with Salmonella gallinarum and fed either control or 200 ppm Pb acetate-supplemented diets was similar, except early in the course of the disease when mortality among Pb-treated birds was marginally greater. In these studies, effects of Pb that could influence immunological homeostasis were demonstrated for MO metabolism of ArA, for production of eicosanoids, and for phagocytosis. There was also the suggestion that these in vitro indices of immune function are related to in vivo disease resistance.

Animals↗