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Biomedical subjects

S O'Driscoll

Publications and source records attributed to S O'Driscoll.

8 recordsLinked to original sources

Analysis of type 1 ryanodine receptor-12 kDa FK506-binding protein interaction.

Although dissociation of the 12 kDa FK506 binding protein (FKBP12)-type 1 ryanodine receptor (RyR1) complex by macrolide immunosuppressants is well documented, effects of many solutes and drugs have not been quantitated. In the current study, the influence of these on binding between solubilised RyR1 and an FKBP12-glutathione-S-transferase fusion protein was analysed using a novel assay. Association between these two proteins is stable, and is not greatly altered by changes in temperature, pH, cations, and endogenous solutes over physiological ranges. Ascomycin, an FK506 analogue, was identified for the first time as a drug which can disrupt the FKBP12-RyR1 complex.

Animals↗

Abduction moment arm of transposed subscapularis tendon.

OBJECTIVE: The purpose of this study was to analyze the effects of the procedure of superior transposition of the subscapularis on the biomechanics of glenohumeral abduction. DESIGN: The abduction moment arms of the subscapularis muscle for the normal attachment and transposed tendon were measured on 10 cadaver shoulders and compared to that for the normal supraspinatus tendon for which it is intended to substitute. BACKGROUND: Superior transposition of the subscapularis tendon has been recommended for surgical repair of massive tears of the rotator cuff, but the effect of this procedure on shoulder biomechanics has not been reported. METHODS: The moment arm about an instantaneous center of rotation was derived, based on the slope of tendon excursion-glenohumeral angle curve. To simulate the insertion of the transposed subscapularis tendon, pseudo-insertion sites were created. RESULTS: Superior transposition of the subscapularis tendon significantly increased its abduction moment arm. The effect was optimal when the simulated insertion site was lateral rather than medial and, to a lesser extent, anterior versus posterior. CONCLUSIONS: The results provided a biomechanical rationale for subscapularis tendon transposition in restoring the loss of abduction strength of the shoulder in a massive cuff tear.

Adult↗

Calmodulin sensitivity of the sarcoplasmic reticulum ryanodine receptor from normal and malignant-hyperthermia-susceptible muscle.

Ca2+ release from sarcoplasmic reticulum (SR) of malignant-hyperthermia-susceptible (MHS) muscle is hypersensitive to Ca2+ and caffeine. To determine if an abnormal calmodulin (CaM) regulation of the SR Ca(2+)-release-channel-ryanodine-receptor complex (RYR1) contributes to this hypersensitivity, we investigated the effect of CaM on high-affinity [3H]ryanodine binding to isolated SR vesicles from normal and MHS pig skeletal muscle. CaM modulated [3H]ryanodine binding in a Ca(2+)-dependent manner. In the presence of maximally activating Ca2+ concentrations, CaM inhibited [3H]ryanodine binding with no differences between normal and MHS vesicles. In the absence of Ca2+, however, CaM activated [3H]ryanodine binding with a 2-fold-higher potency in MHS vesicles. Significant differences between normal and MHS tissue were observed for CaM concentrations between 50 nM and 10 microM. A polyclonal antibody raised against the central region of RYR1 specifically inhibited this activating effect of CaM without affecting the inhibition by CaM. This indicates that the central region of RYR1 is a potential binding domain for CaM in the absence of Ca2+. It is suggested that in vivo an enhanced CaM sensitivity of RYR1 might contribute to the abnormal high release of Ca2+ from the SR of MHS muscle.

Animals↗

Rugby injuries.

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Athletic Injuries↗

Loose-hinge total elbow arthroplasty. An experimental study of the effects of implant alignment on three-dimensional elbow kinematics.

A previous study suggested that the kinematics of a loose-hinge total elbow arthroplasty (TEA) are those of a truly semiconstrained joint. This study addresses the effects of malposition of the implant. The three-dimensional elbow kinematics during simulated active motion were studied in six cadaver specimens using an electromagnetic tracking device. In addition to simulated active elbow flexion, flexion arcs were obtained under an elbow varus or valgus moment, to calculate the structural varus-valgus laxities. The results after four different Coondrad-Morrey TEA positions of implantation were compared with those of the intact elbow. The flexion-extension amplitudes were not significantly decreased after TEA implantation, except with external rotation of the ulnar component, which resulted in a loss of extension. In the intact elbow and after TEA implantation in any position, the mean varus-valgus deviations throughout elbow flexion were in a narrower range than the structural limits imposed by the ligaments (intact elbow) or the TEA hinge design. With internal malrotation of the humeral component over 10 degrees, however, the valgus structural limit was reached and, conversely, the varus limit with external rotation over 10 degrees. The clinical improvement observed with the semiconstrained TEA is derived from the benefits of the less constrained articulation. The proximodistal changes of TEA implantation have no consequence on the kinematic pattern. Rotational malpositioning of either humeral or ulnar component should be avoided, the first because it changes the kinematic pattern toward the structural limits of the implant and, therefore, may lead to excessive stresses at the bone-cement-implant interfaces and to early loosening, and the latter because it causes loss of extension.

Biomechanical Phenomena↗

Osteomalacia in rheumatoid arthritis.

Fifty-four patients with rheumatoid arthritis and severe osteomalacia were found to have considerable bone and general deficiency problems. In 46 of them 14 stress fractures occurred and 32 minimal trauma fractures necessitating admission to hospital. Radiological abnormalities of absorption were found in nearly 25% of the total, implying that dietary factors alone are not always responsible for osteomalacia in patients with rheumatoid arthritis. A high index of suspicion is necessary in the diagnosis of osteomalacia in patients with rheumatoid arthritis, so they may benefit from treatment.

Aged↗