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Biomedical subjects

S Obara

Publications and source records attributed to S Obara.

71 records · Page 4Linked to original sources
PubMed1981

Myocardial oxygen extraction rate under general anesthesia.

Myocardial oxygen extraction rates (R) were measured in dogs anesthetized with eight kinds of anesthetic agents to study the balance between myocardial oxygen consumption and its supply under general anesthesia. The R value for basal anesthesia (pentobarbital), original neuroleptanesthesia (Thalamonal), modified neuroleptanesthesia (droperidol and pentazocine), morphine anesthesia or halothane anesthesia was not significantly different from each other, and similar to those for conscious dogs reported by Merin or Spencer. On the other hand, the R values for ether anesthesia, methoxyflurane anesthesia and enflurane anesthesia were significantly lower than those for the above mentioned anesthesia.

Anesthesia, General↗
PubMed1980

Inotropic effects of non-depolarizing muscle relaxants in isolated canine heart muscle.

The inotropic effects of five non-depolarizing muscle relaxants were examined using an isolated canine heart muscle preparation. Except for fazadinium, all drugs were studied in their commercially available forms. d-Tubocurarine chloride (dTc) and metocurine iodide (MTC) produced dose-dependent decreases in isometric force (F) and the maximum velocity of force development (dF/dt) at concentrations greater than 22.5 x 10(-3) g/L for dTc and greater than 15.0 x 10(-3) g/L for MTC, concentrations which are 3 and 6 times higher than estimated clinical serum concentrations, respectively. Myocardial depression was about 3 times less with MTC than with dTc at equipotent concentrations. The degree of depression in F and dF/dt produced by MTC was almost identical with that produced by phenol, a preservative of MTC, indicating that MTC-induced myocardial depression may be due to the effect of the preservative. Pancuronium bromide (PC) produced a dose-dependent increase in F and dF/dt and decrease in the time to peak force. PC-induced changes in F, dF/dt, and time to peak force were inhibited by administration of propranolol 10(-6) M. The results indicate that PC possesses a positive inotropic effect mediated by beta-adrenergic stimulation. Alcuronium chloride did not change F or dF/dt at concentrations from 5.0 x 10(-3) to 60.0 x 10(-3) g/L. Frazadinium bromide increased F and dF/ dt slightly at a low concentration (1.875 x 10(-2) g/L), but further increases in its concentration returned the values of F and dF/dt to control levels. F and dF/dt were not altered in vitro by concentrations of relaxants that would be anticipated in plasma in vivo in patients given clinically effective doses of 0.3 mg/kg of dTc, 0.1 mg/kg of MTC or PC, 0.2 mg/kg of alcuronium chloride, or 0.75 mg/kg of fazadinium bromide.

Alcuronium↗
PubMed1979

A low-cost, high-precision scope calibrator. A delayed pulse generator with an isolated output.

A simple scope calibrator for voltage and time calibration is described. A square pulse of calibrated amplitude and pulse-width can be triggered in synchrony with CRO sweep, and displayed, with an adjustable delay, simultaneously with biological signals on CRO screen. The output of the device is isolated from ground and has a low output impedance (less than 50 omega for most ranges). Both the amplitude (10 muV--1 V) and pulse-width (0.2--1000 ms) of the outputs cover wide ranges in 1--2--5 steps with an accuracy of less than +/- 1%. The output drifts against changes in power supply and temperature are negligibly small. The circuit is designed to give the high performance at reasonably low cost, and to be built with only the components readily available in the market.

Calibration↗
PubMed1979

Influence of running pace upon performance: effects upon oxygen intake, blood lactate, and rating of perceived exertion.

Three different techniques of middle distance running (fast/slow, slow/fast, and steady pace) have been compared in terms of oxygen intake, blood lactate and ratings of perceived exertion (RPE). The subjects (10 middle distance and long distance male runners) carried out the three patterns of running on the laboratory threadmill according to a randomly ordered sequence, on each occasion covering 1400 m in 4 min. The fast/slow protocol resulted in a rapid and sustained on-transient of oxygen intake, less lactate accumulation, and a lower rating of perceived exertion during the final 2 min of th4 min run. Physiological data thus support coaching impressions of the superiority of the fast/slow protocol.

Adult↗
PubMed1972

Mode of operation of ampullae of Lorenzini of the skate, Raja.

Ampullae of Lorenzini are sensitive electroreceptors. Applied potentials affect receptor cells which transmit synaptically to afferent fibers. Cathodal stimuli in the ampullary lumen sometimes evoke all-or-none "receptor spikes," which are negative-going recorded in the lumen, but more frequently they evoke graded damped oscillations. Cathodal stimuli evoke nerve discharge, usually at stimulus strengths subthreshold for obvious receptor oscillations or spikes. Anodal stimuli decrease any ongoing spontaneous nerve activity. Cathodal stimuli evoke long-lasting depolarizations (generator or postsynaptic potentials) in afferent fibers. Superimposed antidromic spikes are reduced in amplitude, suggesting that the postsynaptic potentials are generated similarly to other excitatory postsynaptic potentials. Anodal stimuli evoke hyperpolarizations of nerves in preparations with tonic activity and in occasional silent preparations; presumably tonic release of excitatory transmitter is decreased. These data are explicable as follows: lumenal faces of receptor cells are tonically (but asynchronously) active generating depolarizing responses. Cathodal stimuli increase this activity, thereby leading to increased depolarization of and increased release of transmitter from serosal faces, which are inexcitable. Anodal stimuli act oppositely. Receptor spikes result from synchronized receptor cell activity. Since cathodal stimuli act directly to hyperpolarize serosal faces, strong cathodal stimuli overcome depolarizing effects of lumenal face activity and are inhibitory. Conversely, strong anodal stimuli depolarize serosal faces, thereby causing release of transmitter, and are excitatory. These properties explain several anomalous features of responses of ampullae of Lorenzini.

Action Potentials↗
PubMed1969

Acceleratory synapses on pacemaker neurons in the heart ganglion of a stomatopod, Squilla oratoria.

The pacemaker neurons of the heart ganglion are innervated from the CNS through two pairs of acceleratory nerves. The effect of acceleratory nerve stimulation was examined with intracellular electrodes from the pacemaker cells. The major effects on the pacemaker potential were an increase in the rate of rise of the spontaneous depolarization and in the duration of the plateau. The aftereffect of stimulation could last for minutes. No clear excitatory postsynaptic potential (EPSP) was observed, however. On high frequency stimulation, a small depolarizing response (the initial response) was sometimes observed, but the major postsynaptic event was the following slow depolarization, or the enhancement of the pacemaker potential (the late response). With hyperpolarization the initial response did not significantly change its amplitude, but the late response disappeared, showing that the latter has the property of the local response. The membrane conductance did not increase with acceleratory stimulation. The injection of depolarizing current increased the rate of rise of the spontaneous depolarization, but only slightly in comparison with acceleratory stimulation, and did not increase the burst duration. It is concluded that the acceleratory effect is not mediated by the EPSP but is due to a direct action of the transmitter on the pacemaker membrane.

Animals↗
PubMed1968

Effects of lithium on different membrane components of crayfish stretch receptor neurons.

Unlike several other varieties of input membrane, that of the crayfish stretch receptor develops a generator potential in response to stretch when all the Na of the medium is replaced with Li. However, Li depolarizes the receptor neuron, the soma membrane becoming more depolarized than that of the axon. During exposure to Li the cell usually fires spontaneously for a period, and when it becomes quiescent spike electrogenesis fails in the soma but persists in the axon. These effects are seen in the rapidly adapting as well as the slowly adapting cells. The block of spike electrogenesis of the soma membrane is only partly due to the Li-induced depolarization and a significant role must be ascribed to a specific effect of Li.

Animals↗
PubMed1968

Effects of some organic cations on generator potential of crayfish stretch receptor.

The generator potential of both slowly and rapidly adapting crayfish stretch receptor cells can still be elicited by mechanical stimuli when all the Na of the bathing medium is replaced by various organic cations. In the presence of tris(hydroxymethyl)aminomethane (Tris), the generator potential is particularly large, about 30-50 % of that in the control saline, while spike electrogenesis of the cell is abolished. Persistence of the generator response is not due to retention of Na by a diffusion barrier, and ionic contributions to the electrogenesis by Ca and Cl can also be excluded. Thus, whereas the electrogenesis of the generator membrane must be due to an increased permeability to monovalent cations, the active receptor membrane appears to be less selective for different monovalent cations than is the receptor component of some other cells, or the conductile component of the stretch receptor neuron.

Acetates↗
PubMed1968

Inhibitory synapses on pacemaker neurons in the heart ganglion of a stomatopod, Squilla oratoria.

The pacemaker neurons of the Squilla heart ganglion are innervated from the CNS through three pairs of extrinsic nerves. One of them, the alpha-nerve, is inhibitory to the heart beat. The effect of alpha-nerve stimulation on the pacemaker potential was examined with intracellular electrodes. Without extrinsic nerve stimulation the membrane potential of the pacemaker cell fluctuated spontaneously. On application of a tetanic train of stimuli to the alpha-nerve the membrane potential was shifted and fixed to a steady level, which with K(2)SO(4)-filled electrodes was near the peak of hyperpolarization after a spontaneous burst, but was less negative with KCl-filled electrodes. The shift of the membrane potential was due to the summated IPSP's. By changing the level of the membrane potential with injection of the polarizing current the IPSP could be reversed in sign, and the size of the IPSP was linearly correlated with the membrane potential level. During inhibition the membrane conductance increased. The increase depended on divalent cation concentrations in the outside medium. In Ca-rich saline the IPSP was greatly enhanced. In Mg-rich saline it was suppressed. The amplitude of antidromic spikes was reduced during inhibition especially when the spike frequency was high.

Aminobutyrates↗
PubMed1967

Electrical properties of the pacemaker neurons in the heart ganglion of a stomatopod, Squilla oratoria.

In the Squilla heart ganglion, the pacemaker is located in the rostral group of cells. After spontaneous firing ceased, the electrophysiological properties of these cells were examined with intracellular electrodes. Cells respond to electrical stimuli with all-or-none action potentials. Direct stimulation by strong currents decreases the size of action potentials. Comparison with action potentials caused by axonal stimulation and analysis of time relations indicate that with stronger currents the soma membrane is directly stimulated whereas with weaker currents the impulse first arises in the axon and then invades the soma. Spikes evoked in a neuron spread into all other neurons. Adjacent cells are interconnected by electrotonic connections. Histologically axons are tied with the side-junction. B spikes of adjacent cells are blocked simultaneously by hyperpolarization or by repetitive stimulation. Experiments show that under such circumstances the B spike is not directly elicited from the A spike but is evoked by invasion of an impulse or electrotonic potential from adjacent cells. On rostral stimulation a small prepotential precedes the main spike. It is interpreted as an action potential from dendrites.

Action Potentials↗
PubMed1967

Pacemaker potentials for the periodic burst discharge in the heart ganglion of a stomatopod, Squilla oratoria.

From somata of the pacemaker neurons in the Squilla heart ganglion, pacemaker potentials for the spontaneous periodic burst discharge are recorded with intracellular electrodes. The electrical activity is composed of slow potentials and superimposed spikes, and is divided into four types, which are: (a) "mammalian heart" type, (b) "slow generator" type, (c) "slow grower" type, and (d) "slow deficient" type. Since axons which are far from the somata do not produce slow potentials, the soma and dendrites must be where the slow potentials are generated. Hyperpolarization impedes generation of the slow potential, showing that it is an electrically excitable response. Membrane impedance increases on depolarization. Brief hyperpolarizing current can abolish the plateau but brief tetanic inhibitory fiber stimulation is more effective for the abolition. A single stimulus to the axon evokes the slow potential when the stimulus is applied some time after a previous burst. Repetitive stimuli to the axon are more effective in eliciting the slow potential, but the depolarization is not maintained on continuous stimulation. Synchronization of the slow potential among neurons is achieved by: (a) the electrotonic connections, with periodic change in resistance of the soma membrane, (b) active spread of the slow potential, and (c) synchronization through spikes.

Animals↗