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Biomedical subjects

S Ofuji

Publications and source records attributed to S Ofuji.

At least 19 recordsLinked to original sources
PubMed1992

The ultrastructural histopathology of eosinophilic pustular folliculitis.

The follicular skin lesions of a patient with eosinophilic pustular folliculitis were investigated by electron microscopy. Pustules in the outer root sheath contained acantholytic keratinocytes with numerous microvilli and features of desmosomal cleavage. The infiltrating eosinophils and neutrophils exhibited autolytic or degenerative changes rather than degranulation. The aggregated tubulo-vesicular structures were associated with the debris of autolytic eosinophils. Multiple, tiny, bubble-like structures enclosed within a membrane were frequently seen in the intercellular space. The intercellular space of the outer root sheath was widened with decreased desmosomal adhesion between the keratinocytes, but no intracellular edema was detectable. The infiltrating lymphocytes, predominantly T-cells with convoluted nuclei, extended cytoplasmic processes to adjacent keratinocytes. Apposition of T-lymphocytes and Langerhans cells was noted. Some keratinocytes in the outer root sheath contained large, sebaceous lipid droplets. No obvious virus particles or other pathogenic agents were detected. It is possible that T-lymphocytes and other immunosurveillance cells are involved in the pathomechanism of eosinophilic pustular folliculitis.

Adult↗
PubMed1990

Papuloerythroderma.

Explore the source record for details and available documents.

Dermatitis, Exfoliative↗
PubMed1979

Binding specificity of rabbit anti-guinea pig epidermal cell sera: comparison of their receptors with those of concanavalin A and pemphigus sera.

Rabbit anti-guinea pig epidermal cell sera (AES) which were prepared by immunizing rabbits with enzymatically dispersed viable guinea pig epidermal cells were shown to react to the intercellular substances of stratified squamous epithelia of guinea pigs and monkeys in a pattern similar to that seen with concanavalin A (ConA) or pemphigus sera (PS) by immunofluorescence. Reciprocal blocking tests were performed on guinea pig lip mucosa after removal of the non-specific binding substances between preincubated substances and subsequently incubated ones. No definite inhibition was noted in the subsequent reactions upon preincubation with AES or PS. However, preincubation with ConA weakened the subsequent reaction with PS, but did not block the reaction with AES. Effects of solvents on the receptors for AES, ConA and PS were also examined. ConA receptor was resistant to both saline solution and ethanol (95% and 99%). PS receptor was labile to both saline solution and ethanol, while AES receptor was labile to ethanol, but resistant to saline solution. These observations suggest that the receptor for AES differs from that for ConA or PS.

Aged↗
PubMed1978

Ultrastructural studies of epidermis in acute radiation dermatitis. Basal lamina thickening and coated vesicles.

Fine structural changes of keratinocytes by X-ray were examined in normal skin area around a lesion of Bowen's disease. The area was exposed to about 6000 r over 28 days. The findings were: a decreased number of desmosomes and microvilli, formation of cytoplasmic vaculoes with or without membrane, perinuclear aggregation of tonofibrils, intracytoplasmic desmosomes and gap junction, cytoplasmic occurrence of dense bodies, lipid droplet and glycogen particles, changes in mitochondria, endoplasmic reticulum and Golgi complex, and deep invagination of the nuclear membrane. Besides these, conspicuous in the present study were, changes in the basal lamina which consisted of multiplication, thickening and occasional detachment from basal cells, and frequent occurrence of coated vesicles along with the basal lamina alteration. Possible exocytotic nature of coated vesicles in X-ray irradiated keratinocytes is discussed.

Aged↗
PubMed1978

Early fine structural changes in human epidermis following application of croton oil.

Early epidermal changes in human subjects following application of 10% croton oil in acetone were studied by electron microscopy. The prominent changes observed at 1 and 5 hours were degeneration of epidermal cells and intercellular edema in the basal and spinous layers. One type of degeneration was homogenization and aggregation of the tonofilaments of keratinocytes at 1 hour, which may explain the presence of dyskeratotic cells at 5 hours. Another type was of a cytolytic nature as evidenced by severe perinuclear edema of keratinocytes and destruction of Langerhans' cells with outpouring of Birbeck's granules in the intercellular spaces.

Cell Membrane↗
PubMed1978

An ultrastructural study of the epidermis in hyperkeratosis lenticularis perstans.

A 60-year-old Japanese man contracted hyperkeratosis lenticularis perstans (Flegel). Biopsy specimens of the keratotic papules on the dorsa of the feet were studied with electron microscopy. An increase in the number of membrane coating granules, keratohyalin granules and tonofilaments was the most prominent feature of the keratinocytes underlying the keratotic papule. However, in the area just above the cone-shaped dermal infiltration, membrane coating granules were absent and keratohyalin granules and tonofilaments were poorly developed.

Arm↗
PubMed1978

A leukotactic factor in the stratum corneum of pustulosis palmaris et plantaris: a possible mechanism for the formation of intra-epidermal sterile pustules.

Previous studies on the chemotactic properties of soluble substances from psoriatic scales demonstrated the presence of complement-derived chemotactic factors (anaphylatoxins) that seem to play a crucial role in the production of typical psoriasiform tissue changes. The crude extract from the stratum corneum of lesions of pustulosis palmaris et plantaris (PPP) was also noted to contain highly chemotactic components for polymorphonuclear leukocytes (PMN's) as compared with a crude callus extract in the present study. The leukotactic factors contained in the major chemotactic fractions isolated by Sephadex G-75 chromatography revealed in vitro characteristics and in vivo biological activities very similar to those of the leukotactic factor derived from psoriasis scale reported in earlier work, i.e. complement cleavage products (C3a and C5a). On the basis of these findings we have reason to believe that pustule formation in PPP occurs through a mechanism that has a strong resemblance to that in pustular psoriasis. We hypothesize further that similar mechanism may be operative in other dermatoses in which the formation of sterile subcorneal pustules is a characteristic feature.

Acrodermatitis↗
PubMed1977

Ultrastructural studies of epidermal lesions in pityriasis lichenoides chronica: occurrence of tubular aggregates and intracytoplasmic desmosomes.

Very early skin lesions from four patients with pityriasis lichenoides chronica were examined by electron microscopy. Aggregates of tubular structures, which resembled those reported in endothelium in lupus erythematosus and are currently being observed in an increasing variety of conditions, were noticed in the epidermal cells of all patients. These cells also showed intracytoplasmic desmosomes, and isolated mitochondria and myelin-like figures in intercellular spaces, in addition to the occurrence of vacuoles and lysosomes in the cytoplasm, dilatation of endoplasmic reticulum and alteration of mitochondria. Occasionally, epidermal cells were seen to be individually filled with fibrils. These ultrastructural findings seem to indicate that in pityriasis lichenoides chronica some injury to epidermal cells occurs at the early stage of pathological processes, as suggested by light microscope studies.

Adult↗
PubMed1977

Characterization of a leukotactic factor derived from psoriatic scale.

A study was made on the in vitro characteristics and in vivo biological activities of a leukotactic factor derived from psoriatic scale (PLF). Sephadex G-75 chromatography showed that PLF eluted in the region of the 12,500 dalton protein marker. It resisted heating, lyophilization, acidity (pH 3) and alkalinity (pH 11), but was destroyed by trypsin digestion. The substance attracted polymorphonuclear leukocytes (PMNs) in vitro; this ability was inhibited by antiserum to C3a and was partially reduced by antiserum to C5. Although PLF showed strong chemotactic activity for PMNs, its activity for mononuclear cells was only mild. Intradermal injection of PLF into man or guinea-pig produced an erythematous response with dense tissue neutrophilia, the time course of which was similar to that of the Arthus response. PLF also provoked increased vascular permeability in guinea-pig skin. Moreover, repeated intradermal injection of PLF to the same site in guinea-pigs led to moderate acanthosis of the overlying epidermis. These observations strongly suggest that transepidermal migration of leukocytes in psoriatic lesions is provoked by PLF, whose activity appears to reflect that of complement cleavage products, particularly C3a, presumably produced by complement activation in the subcorneal region of the epidermis. Furthermore, it is postulated that PLF may be a factor which plays a crucial role in the production of typical psoriasiform tissue changes.

Animals↗