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S Ohkuma

Publications and source records attributed to S Ohkuma.

226 records · Page 13Linked to original sources
PubMed1975

Formation of phagolysosomes containing dextran and Triton WR 1339 in mouse liver.

After injection of Triton WR 1339 and dextran into mice, phagolysosomes containing both compounds were obtained from the liver regardless of the order of injection of these materials. This suggests that phagososomes containing the other material. The recoveries of various lysosomal enzymes differed in phagolysosomes after injection of Triton WR 1339 with or without dextran: recoveries of beta-glucuronidase, beta-N-acetylglucosaminidase and arylsulfatase were high, and that of acid phosphatase was low.

Acetylglucosamine↗
PubMedDate not supplied

Characteristics of nitric oxide-evoked [3H]taurine release from cerebral cortical neurons.

Pharmacological characteristics of [3H]taurine release evoked by nitric oxide (NO) were investigated using mouse cerebral cortical neurons in primary culture. NO generators such as S-nitroso-N-acetylpenicillamine (SNAP) and sodium nitroprusside (SNP) dose-dependently increased [3H]taurine release from neurons. Such stimulatory effects of NO generators were completely abolished by hemoglobin, a NO radical scavenger, indicating that these [3H]taurine releases might be due to NO liberated from SNAP and SNP. Sodium withdrawal from incubation buffer significantly inhibited the SNAP- and SNP-induced [3H]taurine releases, whereas the removal of calcium showed no alterations in the [3H]taurine release evoked by NO generators. Beta-Alanine and guanidinoethane sulfonate, inhibitors of carrier-mediated taurine transport system, inhibited the SNAP- and SNP-evoked releases of [3H]taurine in a dose-dependent manner. These results indicate that the NO-evoked [3H]taurine release from cerebral cortical neurons is mediated by the reverse process of sodium-dependent carrier-mediated taurine transport system.

Animals↗
PubMedDate not supplied

Preparation of blood components with saline-adenine-glucose-phosphate-maltose quadruple-pack system.

A saline-adenine-glucose-phosphate-maltose (SAGP-maltose) quadruple-pack system was devised to improve the conventional method of preparing blood components. One of the features of this method is that high-speed centrifugation was used as the initial spin in the two-step centrifugation of whole blood. This feature permitted the removal of the buffy coat from red cells at the same time the platelets were isolated. Since the platelets were concentrated without forming a button, the damage to the platelets due to mechanical stress in preparation was minimized. Another major feature was the use of the SAGP-maltose solution. Maltose effectively prevented hemolysis during storage in a protein-poor medium for preserving red cells. Judging from the red cell adenosine triphosphate level and morphology score, it should be possible to store red cells for 35 to 42 days in SAGP-maltose preservative. In addition, approximately 25 percent more plasma was collected in the present system than in conventional multiple-pack systems.

Blood Platelets↗