PubMed Health⌕ Search

Biomedical subjects

S Otake

Publications and source records attributed to S Otake.

At least 55 records · Page 3Linked to original sources
PubMed1992

Production of the vacuolation factor of Bacillus cereus isolated from vomiting-type food poisoning.

Vacuole response in HEp-2 cells was induced with culture supernatants of Bacillus cereus strains isolated from outbreaks of vomiting- and diarrheal-type food poisoning grown in rice flour and laboratory media. High vacuole response was obtained with culture supernatants of B. cereus strains isolated from vomiting-type food poisoning grown in cooked rice suspension or on a cooked rice plate, whereas no response was obtained with those of the same strains grown in brain heart infusion and trypto-soya broth media. The vacuole activity appeared only after spore formation of B. cereus. The activity was stable to proteolytic enzymes, heating, and exposing to pH 2.0 and 11.0. Of 124 strains isolated from B. cereus food poisoning that were tested, the vacuole activity was observed by 68 of 110 (61.8%) of the strains isolated from the vomiting-type food poisoning but not by all strains (14 strains) from diarrheal-type ones. Moreover, the vacuole response in the HEp-2 cells was found to be induced by 56 of 76 (73.7%) of the serotype H-1 strains isolated from vomiting-type food poisoning.

Bacillus cereus↗
PubMed1991

[Fast MR imaging of liver metastasis using FLASH and FISP--optimal sequences for T1- and T2*-weighted images].

This paper deals with a study to obtain the optimal sequence of gradient echo (GE) for T1- and T2*-weighted images similar to T1- and T2-weighted images of spin echo (SE). Two GE sequences, fast low angle shot (FLASH) and fast imaging with steady-state precession (FISP), were performed in 15 cases of liver metastasis in various combination of flip angle (FA), repetition time (TR), and echo time (TE). The optimal combinations were summarized as follows: 1) T1-weighted FLASH image with FA of 40 degrees, TR of 22 msec and TE of 10 msec, 2) T1-weighted FISP image with FA of 70 degrees, TR of 100 msec, TE of 10 msec, 3) both T2*-weighted FLASH and FISP images with FA of 10 degrees, TR of 100 msec and TE of 30 msec. Not only to provide the adequate T1- and T2*-weighted images but also to enable breath-holding MR imaging, GE sequences can optionally take place SE in cases of deteriorated images caused by moving artifacts. Other applications support the re-examination and further detailing when required, conveniently rather in short time.

Adult↗
PubMed1991

Anticaries effects of polyphenolic compounds from Japanese green tea.

The dental caries inhibiting effect of the extract from Japanese green tea, one of the most popular drinks in Japan, was studied both in vitro and in vivo. The crude tea polyphenolic compounds (designated Sunphenon) from the leaf of Camellia sinensis were found to effectively inhibit the attachment of Streptococcus mutans strain JC-2 (serotype c) to saliva-coated hydroxyapatide discs. Sunphenon was also inhibitory to water-insoluble glucan formation from sucrose by crude glucosyltransferase of S. mutans JC-2 (c). Among the tea catechins tested, (-)-epigallocatechin gallate and (-)-epicatechin gallate showed the most potent inhibition of the glucosyltransferase activity. Finally, significantly lower caries scores were observed in specific pathogen free rats infected with S. mutans JC-2 (c) and fed a cariogenic diet and/or drinking water containing 0.05% Sunphenon as compared with control rats not receiving polyphenolic compounds.

Adsorption↗
PubMed1991

Protection of rats against dental caries by passive immunization with hen-egg-yolk antibody (IgY).

Hen-egg-yolk antibody (IgY) was prepared against Streptococcus mutans MT8148 serotype c that was cultivated in medium containing sucrose, and it was used in passive caries-immunity studies. Specific pathogen-free rats infected with S. mutans MT8148 (c) and fed with a cariogenic diet containing more than 2% immune yolk powder developed significantly lower caries scores than did the ones infected with the same strain and fed with a diet containing only control yolk powder obtained from non-immunized hens. Similar results were obtained in an experiment with rats infected with S. mutans JC-2 (c) strain. Rats provided a diet supplemented with 0.5% immune water-soluble protein fraction containing S. mutans-specific IgY and challenged with S. mutans MT8148 exhibited significantly fewer caries lesions, compared with control rats on the normal diet.

Administration, Oral↗
PubMed1991

[Recent progress and future prospect of the prolonged extracorporeal lung assist for respiratory failure].

Extracorporeal membrane oxygenation (ECMO) is becoming an accepted therapeutic option for acute respiratory failure in both infants and adults. ECMO has been applied for relatively short-term support and numerous centers have reported satisfactory results with emphasis on patient selection, techniques of cannulation and perfusion, and prevention of complications. To use ECMO for a prolonged support, however, new type of artificial lung and system need to be developed. Most of the membrane oxygenators have the possibility of serum leakage through micropores. To prevent this problem, several dense membrane oxygenators have been developed and clinically used with good gas exchange. A low heparin dosage during ECMO results in reduced bleeding complication. Full systemic heparinization can be avoided during ECMO by using heparin-coated perfusion equipment. Respiratory support by means of pumpless PA-LA extracorporeal membrane oxygenation driven by pulmonary arterial pressure is attractive because of its simplicity and might be suitable for prolonged use. Further studies are necessary to develop an oxygenator for long-term ECMO.

Extracorporeal Membrane Oxygenation↗
PubMed1990

[MR imaging of compressive cervical myelopathy after surgery--high signal intensity of the spinal cord on T2 weighted images].

We reviewed the MR images of 32 patients with cervical myelopathy, showing lesions of high signal intensity in the spinal cord on the sagittal T2 weighted images (T2WI) after surgery: 16 with OPLL; 9 with spondylosis; 4 with disc herniation and 3 with trauma. All images were obtained on a superconducting 1.5 Tesla system. The lesions were classified into five groups, according to the shape and grade of signal intensity on the sagittal T2WI: (I) oval-shaped lesion of signal intensity less brighter than CSF with blurred margin, (II) longitudinal linear-shaped lesion of signal intensity similar to CSF, (III) spindle-shaped lesion of signal intensity similar to CSF, (IV) round-shaped lesion of signal intensity similar to CSF and (V) mixed-types lesions which consisted of group I and II. The present study was summarized as follows: 1) Oval-shaped lesions were seen in the cases of disc herniation and spondylosis with relatively short duration of the symptom, presumptively with relatively short duration of the symptom, presumptively indicative of edema. 2) Most cases of OPLL and spondylosis showed linear-shaped lesions, suggesting necrosis and/or cavitations of the central gray matter. 3) One case of spondylosis developed a spindle-shaped lesion, implicating syringomyelia. 4) Round-shaped lesions were seen in the cases of spinal trauma, suggesting posttraumatic cyst. 5) In a case of mixed-typed lesion examined pre- and postoperatively, only an oval-shaped lesion decreased in size after surgery.

Adult↗
PubMed1990

[CT-guided biopsy of thoracic mass lesions followed by fast stain technique].

We performed CT-guided needle biopsy of 92 thoracic mass lesions using a Rotex-II screw needle. Fast stain technique was performed for an immediate evaluation of the specimen in the last 39 procedures. The overall accuracy of malignancy was 93.2%, and the correct histological typing was obtained in 82.8% of malignancy proven at surgery or autopsy. The method enabled histopathological diagnosis of thoracic lesions, which could not obtained by other diagnostic modalities including fiberoptic bronchoscopy or fluoroscopy-guided biopsy. The true positive rate increased up 10.2% to 91.3% by introducing fast stain technique. A close cooperation between radiologists and cytopathologists was essential for performing this CT-guided biopsy procedure followed by fast stain technique.

Adenocarcinoma↗
PubMed1990

Primary lymphoma of the thyroid: a clinical, histological and immunohistochemical study of 20 cases.

Twenty cases of malignant lymphoma arising in the thyroid gland were studied clinically, histologically and immunohistochemically. Nineteen cases were non-Hodgkin's lymphoma (15 diffuse and four follicular lymphoma) and one was a plasmacytoma. Immunohistochemical analysis of the lymphomas using paraffin-embedded sections disclosed that 17 lymphomas were B-cell type and two were T-cell type. The plasmacytoma was of IgG kappa type. The large majority of the lymphomas were associated with an underlying chronic thyroiditis. The 5-year survival rate of the patients was 70%. An unfavourable diagnosis was more likely when the tumour was diffuse rather than follicular, when it was of diffuse large cell type or of immunoblastic type and when there was cervical lymph node involvement.

Adult↗
PubMed1990

Muscular sarcoidosis: findings at MR imaging.

Twelve patients with muscular sarcoidosis were evaluated with magnetic resonance (MR) imaging: Nine patients had the nodular type and three had the myopathic type of muscular sarcoidosis. The nodular type showed a characteristic appearance on MR images. In each patient with the nodular type, both T1- and T2-weighted axial spin-echo images showed an oval nodule consisting of a star-shaped area of low signal intensity surrounded by an area of high signal intensity. Histopathologic correlation in patients with the nodular type revealed that the central portion of the nodule was fibrotic tissue surrounded by inflammatory granulomatous tissue. In each patient with the myopathic type of muscular sarcoidosis, MR images failed to show any abnormality; the authors hypothesize that this failure correlated with the sparse distribution of epithelioid cell granulomas. Identification of the star-shaped area of low signal intensity may help in the diagnosis of muscular sarcoidosis and the differentiation of lesions associated with sarcoidosis from other soft-tissue masses.

Adult↗
PubMed1990

A clinicopathological study of so-called denture fibroma.

We investigated 60 cases of so-called denture fibroma, most of which occurred in women in their 50s to 70s. The most commonly affected areas were the upper alveolar ridge and the bottom of the alveolabial sulcus, and most of the prostheses responsible were complete dentures. Elevated and tumor-like lesions were most frequent. Histopathologically, about 60% of the lesions were of the fibromatous type. Degeneration and inflammation of the salivary glands were identified in one third of cases.

Adult↗
PubMed1990

[A survey inquiry of clinical examination for 12 years at dental hospital attached to School of Dentistry at Matsudo].

Of the total of 1,371,122 out patients who received the clinical examination in the department of Clinical Pathology. Dental Hospital, Nihon University School of Dentistry at Mastudo for 12 years during April 1, 1977 to March 31, 1989, their clinical examination data were gathered and evaluated. The following results were obtained. 1) The average number of patients who received examination was 1,582.2 per year. 2) The ratio of male to female of the examined patients was 1 to 1.04 respectively. 3) The largest number of patients examined was in their age of 40's in both sexes. 4) Among the various clinical departments, oral surgery department most frequently requested clinical examination followed by head and neck surgery, pedodontics conservative dentistry and anesthesiology departments. 5) Among 7 major clinical examination including general, hematological, biochemical, serological, bacteriological, physiological, and pathological examinations, biochemical evaluation was most frequently requested. In average, 30.5 different examinations were performed per patient. 6) Recently, among pathological and serological examinations, the request of HBs antigen and syphilis examinations were increased.

Adult↗
PubMed1990

[Mitogenic activity of lipopolysaccharide from Bacteroides intermedius against C3H/HeJ mice spleen cells].

Bacteroides intermedius is one of the periodontal disease generative bacteria. Lipopolysaccharide preparation obtained from B. intermedius ATCC 25611 and ATCC 33563 strains was investigated as to Limulus activity observed with Pyrodic (endotoxin specific) reagent, and mitogenic activity in both normal and tolerant mice induced by Escherichia coli. Limulus activities of all LPS's were nearly similar to that of E. coli. LPS's preparation from B. intermedius were found to be strongly mitogenic for C3H/HeN mice spleen cells, whereas no LPS preparations were mitogenic for thymocytes of BALB/c mice. LPS-nonresponsive C3H/HeJ mice spleen cells were found to good mitogenic responses to LPS's from B. intermedius, as well as LPS from B. gingivalis 381 strain. Polymyxin B had remarkable inhibitory effect on the mitogenicity of LPS's from B. intermedius for C3H/HeN and C3H/HeJ mice spleen cells, also that activity of B. gingivalis LPS was inhibited by polymyxin B. The mitogenicity of the LPS from B. intermedius, as measured in C3H/HeJ mice spleen cells, was considerably resistant to the proteolytic effects of proteinase K.

Animals↗
PubMed1989

[IgG, IgA and IgM serum antibodies against lipopolysaccharide from Bacteroides intermedius in periodontal health and disease].

Serum antibody titers to lipopolysaccharide from Bacteroides intermedius (B. intermedius) and Bacteroides gingivalis (B. gingivalis) were measured by enzyme-linked immunosorbent assay in serum samples from subjects with healthy gingiva and patients with periodontitis. The IgG antibody titers to LPS from B. gingivalis were significantly higher in the patients group, compared with healthy controls. No such difference was found between IgG activity to LPS from B. intermedius in the healthy and the periodonitis. Likewise, no significant difference in specific IgA level was found between the group with healthy gingiva and the patients group, both in LPS from B. intermedius and B. gingivalis. The IgM antibody levels to LPS from B. intermedius and B. gingivalis were low in subjects of patients and controls.

Antibodies, Bacterial↗
PubMed1989

[Toxicity of Bacteroides intermedius surface extracts against mouse splenic lymphocyte].

The purpose of the present study is to clarify in vitro toxicity of surface sonic extracts from Bacteroides intermedius (B. intermedius) ATCC25611, B. intermedius ATCC 33563 and Bacteroides gingivalis (B. gingivalis) 381 against mouse splenic lymphocyte. Especially the surface extracts from B. intermedius ATCC 25611 were fractionated by high performance liquid chromatography (HPLC) on a column of DEAE. The following results were obtained: 1) The surface extracts from B. gingivalis 381 and B. intermedius ATCC 25611 presented remarkable lymphotoxicity, but B. intermedius ATCC33563 did not have it. 2) The surface extracts from B. intermedius ATCC 25611 was separated to 6 fractions by HPLC. 3) Two fractions isolated from B. intermedius ATCC 25611 surface extracts observed remarkable lymphotoxicity, and the one of their fractions, possibly including fimbriae showed a remarkably higher toxicity against mouse splenic lymphocyte.

Animals↗
PubMed1989

[High resolution computed tomography of diffuse interstitial pneumonia during treatment].

HRCT was carried out in twenty patients with diffuse interstitial pneumonia: 13 cases of IIP, 3 of BOOP, 2 of drug-induced pneumonia, 1 of rheumatoid lung and acute interstitial pneumonia of unknown origin. With special attention to inflammatory activity, the patients underwent HRCT periodically during the treatment. Correlative investigation between HRCT image and grade of accumulation in 67Ga scintigraphy was also performed. Response to steroid therapy was clearly reflected on HRCT image, that was shown as decreasing pulmonary density or thinning of honeycomb wall. HRCT is considered to be useful in assessing the activity of diffuse interstitial pneumonia.

Aged↗
PubMed1989

[Roentgenologic evaluation of indrawn parietal pleura].

Among linear densities extending out from the lateral margin of a peripheral mass to the pleural surface, a thin line with a small triangular density at the pleural surface is considered to be contiguous layers of indrawn visceral pleura with a small amount of fluid between layers. We agree that the thin line represents contiguous layers of indrawn visceral pleura, but we believe that the triangular density at the pleural surface is not formed by a small amount of pleural effusion, but by indrawn parietal pleura accompanying extrapleural soft tissue of the chest wall. A trapezoid or a belt shaped soft tissue density between a peripheral mass contiguous to the visceral pleura and the chest wall are also signs of indrawn both visceral and parietal pleurae in the presence of adhesion of the pleura over the mass.

Aged↗