Biomedical subjects

S Othman

Publications and source records attributed to S Othman.

39 records · Page 3Linked to original sources

PubMed1987

Effects of food and gender on the pharmacokinetics of methotrexate in children.

The effects of food and gender on the oral absorption of methotrexate (MTX) in 16 Jordanian children were investigated. Plasma levels of MTX were measured by a fluorescence polarization immunoassay. The extent of absorption and the rate of elimination of the drug were determined in both males and females, before and after meals and data were statistically analyzed using two-way analysis of variance. The results showed great variability in individual MTX Cmax, t1/2, Kel. and AUC. Although no statistically significant differences were found in the pharmacokinetic parameters of the drug among the groups, apparent differences were observed in the mean values of these parameters.

Administration, Oral↗

PubMed1986

Effect of forskolin on cAMP accumulation and ketogenesis in isolated hepatocytes.

The effect of forskolin on ketogenesis and cAMP accumulation was studied in hepatocytes from euthyroid and hypothyroid rats. Forskolin stimulated ketogenesis, cAMP production and potentiated glucagon-stimulated cAMP accumulation on both euthyroid and hypothyroid groups. The ketogenic effect of glucagon was inhibited by forskolin in both groups. Also, forskolin, glucagon and methylisobutylxanthine (MIX) combined did not significantly increase ketogenesis.

1-Methyl-3-isobutylxanthine↗

PubMedDate not supplied

Liquid chromatographic determination of glibenclamide in human plasma.

The oral hypoglycemic agent glibenclamide was determined in human plasma by liquid chromatography (LC). Samples, with internal standard added, are extracted with dichloromethane. The organic phase is evaporated, and the residue is reconstituted in mobile phase for injection onto the LC column. Intra- and inter-day variability of the method was assessed at high and low levels of the drug. Although coefficients of variation were similar for both intra- and inter-day studies at both levels, CVs were smaller at the higher concentration level. Recovery of the drug was good at both high and low levels. The minimum level of detection was 5 ng/mL.

Chromatography, Liquid↗