Biomedical subjects

S P Navarro

Publications and source records attributed to S P Navarro.

4 recordsLinked to original sources

PubMed1998

K phenotyping using a PK-7200 automated analyzer.

K (Kell) is one of the most immunogenic of the red blood cell (RBC) antigens. In order to select K- RBC units, we developed K phenotyping on the Olympus PK-7200 equipment to save labor, time, and costs. The Olympus PK-7200 is fully automated equipment used primarily for blood typing and syphilis screening. We tested 3,587 blood donor samples in EDTA using a commercial anti-K serum diluted in HP Hemagen Power Solution(1:40). The equipment was set to prepare a 1.7% RBC suspension in bromelain and to dispense 25 microL of the mixture (diluted serum and HP Hemagen Power Solution) in terraced microplates. After mixing, the microplates were incubated for 1 hour at 30 degrees C. Reading was performed by a C.C.D. camera and the results were automatically transferred to the mainframe computer. We found 185 K+ blood samples and 3,402 K- samples. Four samples, K+ by the PK-7200, were confirmed as K- by tube test. The use of bromelain with the PK-7200 may have caused the falsely positive tests. The Olympus PK-7200, used for K phenotyping, saves labor time and costs. It also reduces handling and thus promotes less contamination risk for laboratory personnel.

Journal Article↗

PubMed1991

Effect of decentralization and partial outflow obstruction on cholinergic receptors in urinary bladder of rabbit.

In this study we have examined the cholinergic receptor density on the detrusor smooth muscle from rabbits with decentralized autonomous bladders and animals with partial outlet obstructions using (3H) quinuclidinyl benzilate ([3H] QNB). Decentralized spinal bladders were obtained from rabbits made paraplegic by a twenty-minute ischemic lesion to the lumbar and sacral spinal cord. Bladder tissues from these rabbits were studied from one, two, five, and nine-day intervals after the ischemic lesion. The affinity of the muscarinic binding sites for (3H) QNB was virtually unchanged at any of the time intervals, while the total Bmax was significantly reduced at one and two days but recovered to normal levels by the fifth and ninth day after the ischemic lesion, provided the bladder had been effectively manually emptied on a regular basis. In a parallel study animals with a partial outlet obstruction or spinal animals with marked bladder distention at the time of sacrifice showed that the cholinergic receptor density was significantly reduced.

Animals↗

PubMed1988

The response of autonomic receptors to castration and testosterone in the urinary bladder of the rabbit.

The effects of castration and testosterone on the autonomic receptor density and contractility in the urinary bladder smooth muscle of male rabbits were compared to untreated animals. Four groups of rabbits were studied over a similar time span with Group 1 animals serving as the untreated controls. Two groups (Groups 2 and 3) were castrated 28 days prior to sacrifice, Group 2 animals received corn oil for 14 days, and Group 3 animals received testosterone, 10 mg./day, for 14 days. The Group 4 animals were non-operated and received testosterone 10 mg./day for 14 days. Ligand saturation binding studies for alpha adrenoceptors in the bladder base and proximal urethra were performed with [3H]dihydroergocryptine ([3H]DHE). Muscarinic cholinergic receptors (MChR) were assayed with [3H]quinuclidinyl benzilate ([3H]QNB) and beta adrenoceptors with [125I]iodocyanopindolol ([125I]CYP) on the detrusor smooth muscle. Castrated Group 2 animals showed no significant change in receptor density with either [3H]QNB or [125I]CYP in detrusor muscle, but did exhibit a significant reduction (59%) of alpha adrenoceptors in the bladder base-urethra. The testosterone treated castrate and testosterone treated non-operated animals had significant increases in the MChR density, but no change in the alpha adrenergic, or beta adrenergic receptor density as compared to untreated controls. Cumulative dose response contractile studies were performed with carbachol on detrusor muscle strips and with phenylephrine on bladder base strips in isolated organ baths. The contractile studies on muscles from Groups 1, 2 and 3 showed no change in the ED50 or maximal contractile strength between control, castration or testosterone treated castrated animals. The ratio of wet bladder weight as compared to total body weight between each of the treatment groups showed a slight increase in both of the testosterone treatment groups. It was concluded that castration down regulates the alpha adrenergic receptors of the bladder base, while testosterone treatment increases the density of MChRs, and increases the ratio of the bladder to total body weight. Although no contractile changes were observed in the bladder base tissue it is conceivable that longer chronic testosterone deficits might ultimately affect the bladder outlet resistance in the male because of the reduced alpha adrenergic receptor density.

Animals↗

PubMed1985

Quantitation and stability of cholinergic receptors in human bladder tissue from post surgical and postmortem sources.

Ligand binding for cholinergic muscarinic receptors with (3H) quinuclidinyl benzilate was performed on human detrusor smooth muscle from post surgical and postmortem sources. Following cystectomy, detrusor smooth muscle was serially sampled over an interval of 0 to 9 hours. (3H) quinuclidinyl benzilate binding on individual samples indicated that the total receptor density remained relatively unchanged. Postmortem specimens obtained from patients ranging in age from 1 day to 82 years were also assayed and the cholinergic receptor density was found to range between 18.4 to 82.1 fmoles/mg. of protein. All of the affinity constants from both sources were also in a relatively narrow range of 20.0 to 99.0 pmolar. The results of this study provide evidence for the usefulness of postmortem human tissue for evaluating cholinergic receptors in normal and dysfunctional bladder states.

Adolescent↗