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Biomedical subjects

S P Rassanov

Publications and source records attributed to S P Rassanov.

7 recordsLinked to original sources
PubMed1991

[The characteristics of Staphylococcus aureus peptidoglycan in the spectrum of the reactivity of human lymphocytes to bacterial peptidoglycans].

The sensitivity of lymphocytes of healthy persons to S. aureus peptidoglycan as compared with that to the polyclonal stimulator zymosan C3b and peptidoglycans of other bacteria (Streptococcus faecalis, Escherichia coli, Bacterium bifidum) was analyzed with a test system permitting the determination of specific reactivity to peptidoglycans. The analysis showed that at the peak of luminol-dependent chemiluminescence (25-30 minutes) individual reactivity to S. aureus peptidoglycan varied within wide limits (the coefficient of lymphocyte stimulation was 1.4-9.6, 3.5 +/- 0.6), exceeding sensitivity to other bacteria, as well as the values obtained in the negative control. The conclusion of the wide spread of sensitization to S. aureus peptidoglycan and the possibility of using this preparation for the study of cell-mediated immunity reactions was made.

Bifidobacterium↗
PubMed1989

[Reactivity of human lymphocytes to yeast-like and mold fungi].

The capacity of the allergens of mold fungi (Rhizopus nigricans, Mucor pusillus, Alternaria tenuis, Cladosporum herbarum, Fusarium oxysporum) and yeast-like fungi (Candida albicans) to induce the production of lymphokins by human lymphocytes was studied. All these preparations were active in reactions with lymphocytes obtained from adult donors, but did not activate lymphocytes of newborns (obtained from umbilical blood). In equal doses (10 micrograms/ml) C. albicans allergen was more active than the preparations of mold fungi. The capacity of bacterial allergens to stimulate human lymphocytes was found to be either more pronounced (in Staphylococcus aureus, Streptococcus pyogenes) than that of the preparation of C. albicans or equal to it (in Streptococcus faecalis). The results thus obtained may be regarded as the manifestation of immunological contacts with the antigens of different microorganisms, as well as the evidence of the immunological nature of lymphocytic reactions to preparations intended for use in clinical allergology.

Adolescent↗
PubMed1989

[C3b-mediated chemiluminescence of human lymphocytes].

Data are presented concerning peculiarities of human lymphocyte luminol-dependent chemiluminescence stimulated with C3b-opsonized and native zymosan. The response was maximal by the 60th min and 4-17 exceeded the results of the negative control. The C3b-opsonization considerably potentiated the lymphocyte activating zymosan's properties and the rate of reaction/maximal level at the 10th min; the effect of phytohemagglutinin reached max by the end of the 1st min in the same tests. The functional significance of the CR- and lectin-mediated structures of lymphocytes is discussed.

Complement C3b↗
PubMed1988

[Reactivity of the blood phagocytes in patients with chronic bronchitis].

Bactericidal and migration activity of neutrophils was investigated in 53 patients with chronic bronchitis by means of a spontaneous and induced (6 stimulators) NBT-test and in 18 patients by means of skin fenestrae. A spontaneous NBT-test (sNBT-test) was shown to reflect the activity of an inflammatory bronchial process and to decrease by the end of therapy indicating complete remission. A stimulation index was in reverse correlation with sNBT-test indices. Response of neutrophils to various stimuli leveled down with an increase in sNBT-test indices. In an inflammatory bronchial process inhibition of neutrophil migration into a zone of inflammation was noted, particularly in patients with lingering exacerbation. Dependence of a stimulating effect on a sNBT-test value and disorder of neutrophil migration activity should be taken into account in administration of one or another immunostimulator.

Adjuvants, Immunologic↗
PubMed1987

[Reactive chemiluminescence of human lymphocytes during phytohemagglutinin stimulation].

The luminol-mediated chemiluminescence of highly purified human peripheral blood lymphocytes has been studied. The incubation of lymphocytes with phytohemagglutinin has lead to dose-dependent chemiluminescence, most pronounced by the end of the first minute. The kinetics of the reaction does not depend on the dose of the stimulant, the concentration of lymphocytes or the presence of mononuclear phagocytes. Monocytes (adhering cells) are not stimulated with phytohemagglutinin though their presence in the total fraction of mononuclear blood cells enhances the chemiluminescence of lymphocytes.

Cell Separation↗