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Biomedical subjects

S P Sharma

Publications and source records attributed to S P Sharma.

At least 91 records · Page 5Linked to original sources
PubMed1984

Cytochemical interaction of nucleolus and cytoplasm in the Purkinje cells of senile white rats under the influence of centrophenoxine.

Senile white rats were treated with centrophenoxine at a dosage of 100 mg/Kg body weight/day for 60 days intraperitoneally. Sections of variously fixed and embedded cerebella were studied cytochemically to note the effect of the drug on the senile Purkinje neurons. The nucleolus was found to be hyper-active, as evidenced by the processes of budding and extrusion. A frank regeneration of the Nissl patches along with an increase in alpha-esterase and decrease in the activity of acid phosphatase and simple esterase was noted in the Purkinje cells after 60 days' treatment. It is suggested that the drug exerts its positive effects by regenerating the general cytoplasm and by revitalizing the nucleocytoplasmic interactions in the senile Purkinje cells.

Acid Phosphatase↗
PubMed1984

Changes in nucleic acid content in aging Drosophila bipectinata.

DNA content decreases during first 20 and 25 days of survival in male and female flies of Drosophila bipectinata respectively. RNA as well as RNA/DNA content show a similar trend in males while in females both rise significantly during the first 5 to 10 days of survival followed by a steady decline till day 25. The study suggests that decreasing nucleic acid content of aging D. bipectinata is accompanied by the decline in protein synthesizing capacity.

Aging↗
PubMed1984

Age-related changes in nucleic acids and protein in Callosobruchus maculatus Fabr. (Coleoptera).

The mean life span of Callosobruchus maculatus male is 7 days and female is 5 days at 30 +/- 1 degrees C. Age-related study on C. maculatus shows that RNA and protein contents gradually decline with age in male bruchids, whereas in females initially these increase up to the second day of post-emergent life and subsequently decrease. DNA increases up to the third day in males and the fourth day in females representing post-emergent growth, and then declines sharply on the sixth day. The RNA and protein contents per unit DNA decrease steadily in males; however, in females both are highest during the first 2 days, which synchronizes the egg-laying peak. These observations have been found to be statistically significant (p less than 0.01) and suggest that aging is accompanied by a decrease in both RNA and protein synthesis potential.

Aging↗
PubMed1984

Chemoprophylaxis with diminazene aceturate in experimental Babesia bigemina infection in cattle.

Four-month-old fully susceptible cross bred calves were immunised against Babesia bigemina infection by treating them, during the prepatent period, with one or two doses of diminazene aceturate at 3 mg (kg bodyweight)-1. After 45 days the recovered calves were challenged with the homologous strain of B bigemina infected blood. The ensuing host responses, body temperature, parasitaemia, haemoglobin, packed cell volume, haemoglobinuria, capillary agglutinating antibodies and mortality, were studied. The four untreated control calves developed a severe form of the disease with classical symptoms and two died. Calves of both groups which recovered resisted the challenge infection with a mild relapse in the immunised group, thus indicating a state of premunition. The effect of one dose of diminazene was sufficient to suppress all the clinical effects of the disease.

Amidines↗
PubMed1983

Effect of butylated hydroxytoluene on the life span of Drosophila bipectinata.

The median and the maximum life spans of Drosophila bipectinata increased after feeding with various concentrations (10 microM, 10(2) microM and 10(3) microM) of butylated hydroxytoluene (BHT). Insects fed on the diet supplemented with 10(3) microM BHT had decreased rate of lipid peroxidation (measured by thiobarbituric acid test) with respect to the controls. It is suggested that the antioxidant BHT, which scavenges free radicals, prolongs the life span of Drosophila.

Aging↗
PubMed1983

Age-related protein changes in the banana fruitfly.

Protein analyses from head and body revealed changes in content and electrophoretic banding pattern during aging of the banana fruitfly, Zaprionus paravittiger. Total protein content of head and body increased during the first 13 days of post-emergent life, followed by decline with advancing age. Electrophoretic studies indicate that certain proteins present in newly emerged flies disappeared with the advancing age while a few specific proteins appear and accumulate as the total protein content decreases during post-reproductive period.

Aging↗
PubMed1983

Sensitivity of guaiac-impregnated cards for the detection of colorectal neoplasia.

The sensitivity of the guaiac-impregnated card, hemoccult II, for the detection of colorectal neoplasia was tested in 213 patients undergoing elective colonoscopy over a 10-month period. Contrary to statements in standard texts as to the high sensitivity of guaiac-impregnated cards for the detection of these tumors, our results demonstrate only a 52% sensitivity for colorectal carcinoma and a 23% sensitivity for adenomatous polyps over 1.0 cm in diameter. We compare these results to other studies testing hemoccult sensitivity in the presence of colorectal neoplasia. This relatively low sensitivity rate emphasizes that the use of guaiac-impregnated cards alone does not exclude colorectal carcinoma in any patient.

Adult↗
PubMed1981

Sarcocystosis in goats: clinical signs and pathologic and hematologic findings.

Clinical sarcocystosis was studied in 37 goats after inoculation with graded doses of sporocysts of Sarcocystis capracanis. Eight uninoculated goats served as controls. Clinical response varied with the dose. Goats inoculated with 10-40 million sporocysts died between 11 and 13 days after inoculation (DAI), from interstitial pneumonia, vasculitis, and necrosis of mesenteric lymph nodes. All goats inoculated with 100,000 or 1 million sporocysts died between 19 and 23 DAI; clinical signs were anorexia, fever (40-41 C), anemia, and weight loss. Four of 4 goats inoculated with 50,000 sporocysts and 1 of 4 inoculated with 10,000 sporocysts died 24, 28, 39, 68, and 61 DAI, respectively. Goats inoculated with 1,000 sporocysts and uninoculated goats remained clinically normal. After day 18 and before day 68, packed cell volume and hemoglobin content decreased to as low as 11% and 3.6 g/dl, respectively. Alanine aminotransferase and lactic dehydrogenase activities were inconsistently increased. Blood urea nitrogen and bilirubin values were increased, reaching as high as 63 mg/dl and 10 mg/dl, respectively. Histologically, thymic atrophy, vasculitis, hepatitis, cholangitis, myocarditis, generalized myositis, and encephalomyelitis were the main microscopic findings. The cause of the anemia in goats that died after day 19 was not determined.

Animals↗
PubMed1981

Age-dependent changes in esterases of Callosobruchus maculatus Fab. (Bruchidae: Coleoptera).

Age-dependent changes, quantitative as well as qualitative, in esterase activity were found by assaying crude extracts of Callosobruchus maculatus. There is increase in esterase activity in both the sexes with advancing age in bruchids. Esterase from old bruchids showed several additional electrophoretic bands which did not appear in assays of young bruchids; in addition there is an increase in staining intensity of the bands. A rapid increase in esterase activity during the last days of life may be responsible for producing energy used in various metabolic processes in an attempt to escape death.

Aging↗
PubMed1981

Quantitative survival of Toxoplasma gondii tachyzoites and bradyzoites in pepsin and in trypsin solutions.

Bradyzoites of Toxoplasma gondii survived in pepsin solutions and in trypsin solutions. After 120 minutes in trypsin solution at 37 C, infectivity of bradyzoites for mice was reduced 100-fold, whereas in pepsin solution there was no reduction in infectivity. Tachyzoites survived in trypsin, but after 15 and 60 minutes' incubation at 37 C, there was a 100,000-fold reduction in infectivity titer. Toxoplasma gondii was demonstrated in mice inoculated with experimentally infected goat brain digested in pepsin and in trypsin solutions, but not in mice inoculated with undigested brain of the same goat. Seemingly, tissues should be digested in pepsin solution to isolate T gondii from chronically infected animals.

Animals↗
PubMed1981

Characterization of Toxoplasma gondii isolates from an outbreak of toxoplasmosis in Atlanta, Georgia.

Toxoplasma gondii was isolated from tissue of 5 of 7 cats and of 4 of 4 field mice trapped in a riding stable in Atlanta where an outbreak of toxoplasmosis had occurred in persons. Dye-test antibodies were not found in serum of 4 cats and 4 field mice shown to be infected with T gondii by mouse inoculation. The pathogenicity and infectivity of the 9 isolates of T gondii from cats and mice were compared in mice with the same characteristics of an isolate from a person who acquired toxoplasmosis in association with the stable. Oocysts of all 10 isolates were more pathogenic than cysts of the same isolates. Epidemiologic implications of isolates from cats and mice are discussed.

Animals↗