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Biomedical subjects

S Peach

Publications and source records attributed to S Peach.

7 recordsLinked to original sources
PubMed1979

Definitive identification methods: antibiotic sensitivity and microtube system.

The definite identification of species and subspecies of an anaerobe implies certain difficulties, such as the poor and slow fermentation of carbohydrates under anaerobic conditions and the fact of having many keys in common. As a result several tests are required and as the number of tests increases the chances of error and the number of variables also increase. Hence specific methods have been developed in order to try and obtain satisfactory results. The A. refers his experience and judgement on V.P.I. and A.P.I. and on identification based upon antibiotic sensitivity spectrum. When properly used, such techniques enable the species and subspecies of non-sporing anaerobes to be more easily identified.

Anaerobiosis↗
PubMed1979

Intestinal bacterial flora in normal adults in the U.K.

The A. reports on the composition of the bacterial flora being present all through the intestine of normal adults in the United Kingdom, listing the number and families of the aerobes and anaerobes occupying the various portions of the alimentary tract. Emphasis is then laid on a comparison between the normal "British" flora in the colon and rectum, where the ratio of anaerobes to aerobes is 100 to 1, and the flora found in normal adults in Africa, India and Japan, stressing the major differences in the numbers of clostridia and non-sporing anaerobes. The A. finally highlights the importance of the factors governing the intestinal bacterial ecology, as well as the serious consequences likely to arise from the alteration of such flora.

Actinomycetaceae↗
PubMed1978

Mucosal-associated bacterial flora of the intestine in patients with Crohn's disease and in a control group.

The bacterial flora intimately associated with the intestinal mucosa of patients with Crohn's disease has been examined and compared with the mucosal flora of a control group. Specimens were obtained at operation from patients undergoing intestinal surgery. Whole thickness intestinal sections were taken from the diseased segment and from a portion of uninvolved intestine from patients with Crohn's disease as well as from a control group. A section of each specimen was examined histologically. Twenty-two specimens of Crohn's tissue (12 ileum, 10 colon) and 46 control samples from small and large bowel were examined using strictly anaerobic bacteriological techniques in an anaerobic chamber. Organisms were grown under both aerobic and anaerobic conditions. A mucosal flora was found to exist in all the large bowel samples and in three-quarters of the small bowel samples. It was qualitatively similar in all the samples, consisting mainly of Gram positive bacteria, aerobic Gram negative rods and bacteroides. Greater numbers of bacteria were associated with colonic tissue (10(7) - 10(8) per g) than with tissue from the jejunum (10(3) - 10(4) per g). This difference was statistically significant (p<0.03). Samples from the terminal ileum were quantitatively intermediate between jejunum and colon. There was no statistical difference in the numbers of bacteria associated with Crohn's tissue compared with histologically normal tissue from the same patients and from the control group of patients. Among the bacterial isolates, however, Enterobacteria were more commonly associated with Crohn's tissue.

Aerobiosis↗
PubMed1976

Faecal steroids and bacteria and large bowel cancer in Hong Kong by socio-economic groups.

In a study of three socio-economic groups in Hong Kong, the high income group had a high faecal concentration of bile acids, especially the dihydroxy bile acids, compared to the low income group. The faecal bile acids were also more highly degraded. The faecal flora contained more bacteroides and fewer eubacteria. Very few of the clostridia able to dehydrogenate the steroid nucleus were isolated. An epidemiological study based on street blocks indicated that the high income group also have a higher incidence of cancer of the large bowel and of the breast. The results are discussed in terms of theories on the aetiology of large bowel cancer.

Adult↗
PubMed1976

Clostridia isolated from faeces.

Clostridia were isolated from human faecal samples from England, Scotland, Wales, USA, Hong Kong, Uganda and Japan. Strains were identified on the basis of various biochemical reactions and on the type of fatty acid produced from glucose. Analysis of these acids was by gas-liquid chromatography. Clostridium paraputrificum was common in stool samples from England, Scotland, Wales, and USA, but rare in samples from Hong Kong, Uganda and Japan.

Adult↗
PubMed1975

Antibiotic-disc tests for rapid identification of non-sporing anaerobes.

The sensitivity patterns to a number of antibiotic-impregnated discs of 126 strains of Gram-negative anaerobes and 36 strains of Gram-positive non-sporing anaerobes from various sources have been examined. The sensitivity patterns shown by Bacteroids fragilis and Bacteroides melaninogenicus were useful for the rapid identification of these organisms. Consistent and useful sensitivity patterns were not shown by the Gram-positive anaerobes.

Anaerobiosis↗