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Biomedical subjects

S Pepeljnjak

Publications and source records attributed to S Pepeljnjak.

9 recordsLinked to original sources
PubMed1991

Effect of ochratoxin A on brush border enzymes of rat kidney.

Ochratoxin A was given orally at 60 microgram/kg body weight in neutral olive oil to Fischer rats for 30 days, at which time they were killed. Clinical state, weights of animals and of their organs and urea and creatinine concentrations were not affected during the exposure period. Significant increases in the activity of enzymes in urine were found: 60% increase in alanine aminopeptidase, 45% increase in gamma-glutamyl-transferase and 90% increase in alkaline phosphatase. These changes indicate early pathological changes in the kidney. Relatively small amounts of the toxin thus affect kidney membrane cells.

Alkaline Phosphatase↗
PubMed1990

Ochratoxinogenicity of Aspergillus ochraceus strains from nephropathic and non-nephropathic areas in Yugoslavia.

Mycological analyses of 855 samples of stored grains and dried meat collected in period 1980-1987 from individual households in the nephropathic and wider non-nephropathic area in SR Croatia in Yugoslavia showed 10% of samples to be contaminated with Aspergillus ochraceus. Ability to produce ochratoxin A (OA) was tested in 70 samples (27 from nephropathic areas and 43 from non-nephropathic areas). The detection was carried out under UV-light (365 nm) (light blue fluorescence) and 6 OA-producers were found. A biosynthetic procedure on liquid nutritional substrate with saccharose and yeast extract as well as a method using wet crushed wheat revealed that 37% of the samples from a nephropathic area, and 35% of the samples from a non-nephropathic area produce OA. In the nephropathic area 1/10 strains was a strong producer of OA (concentration crushed wheat 135 mg/kg, and 240 mg/l on YES liquid substrate), 1/10 strains was a moderate producer (concentration 16.6 mg/l and 0.07-7.0 mg/l and 0.1-10.4 mg/kg). Among the strains isolated from a wider non-nephropathic area no strong producers of OA were found, but 2/15 strains were moderate producers of OA (concentration of OA 20.4-27.0 mg/l and 15.0-33.7 mg/kg). The other strains, 8/10 on the crushed wheat and 13/15 on the liquid substrate, were weak producers of OA with concentrations of OA between 0.2-9.0 mg/l and 0.2-10.0 mg/kg with the two methods respectively.

Animals↗
PubMed1985

The mycotoxicological chain and contamination of food by ochratoxin A in the nephropathic and non-nephropathic areas in Yugoslavia.

Research was carried out on the distribution of moulds on cereals in vegetation and in storerooms in the period from 1974 to 1981 and on ochratoxin (OA) in stored maize and wheat as well as residues of OA in the organs of swine in the nephropathic and non-nephropathic areas in the SR of Croatia, Yugoslavia. It was shown that moulds belonging to toxogenic species contaminate cereals in vegetation to an approximately equal degree in both areas (Penicillium 6.6-20.0%, Aspergillus 2.5-6.6% and Fusarium 80-100%). Stored cereals were contaminated by species of Penicillium 75-82.8%, Aspergillus 2.5-27.1% and Fusarium 57.1-82.5%, with a somewhat higher degree of contamination in the nephropathic area. Ochratoxin A occurs on cereals on the whole territory of the SR of Croatia, but average concentrations are higher in the nephropathic area (45% of the positive findings of OA were over 2 mg/kg). Residues of OA in the kidneys (16-77 micrograms/kg), liver (0-21 micrograms/kg) and blood (36-77 micrograms/l) were detected in 38 organs taken from swine in the nephropathic area, but this toxin was not found in the 6 samples taken from the non-nephropathic area. In the same organs histopathological changes were found in the kidneys (interstitial nephritis with parenchymal degeneration of the distal parts of the tubular epithel) and liver (interstitial hepatitis with fatty degeneration of the liver).

Animal Feed↗
PubMed1985

Flavonoid content in propolis extracts and growth inhibition of Bacillus subtilis.

Thirty eight propolis samples were collected in several regions of SR Croatia differing in climate and vegetation. Amounts of 3,5,7-trihydroxyflavone and of 5,7-dihydroxyflavonone were determined chromatographically in individual propolis samples. Concentrations of each constituent were correlated with the growth inhibitions of Bacillus subtilis (IP-5832).

Bacillus subtilis↗
PubMed1978

Rapid thin layer chromatographic method for determining aflatoxin B1, ochratoxin A, and zearalenone in corn.

A multimycotoxin thin layer chromatographic method is described for the analysis of corn. Aflatoxins are extracted from the samples with acetonitrile-water, and sodium bicarbonate is added to separate the acidic ochratoxin from zearalenone and aflatoxin B1. After chloroform extraction, 1N NaOH is added to separate zearalenone and aflatoxin B1. The separated mycotoxins are spotted on TLC plates, which are then examined under ultraviolet light. The following recoveries (%) were obtained for corn samples: aflatoxin B1 71, ochratoxin A 87, and zearalenone 85. The limits of detection for the respective mycotoxins were 2, 40, and 200 ppb.

Aflatoxins↗