PubMed Health⌕ Search

Biomedical subjects

S Perdue

Publications and source records attributed to S Perdue.

26 records · Page 2Linked to original sources
PubMed1980

Discrimination of T, B, and null lymphocytes by electronic sizing.

T, B, and null lymphocytes isolated by sheep red blood cells (SRBC) rosetting or adherence to a nylon wool column had distinct size differences according to Coulter counter size analysis. The diameters in microns were as follows: B = 6.42, T = 6.76, null = 7.16, granulocytes = 8.42, and monocytes = 9.02. Coulter size distributions of cell preparations were compared to the proportions of cells with E, EAC, and surface immunoglobulin (SIg) markers in these preparations as well as their reactivity to HLA--DR antisera and rabbit anti-B-lymphocyte antisera. Cell preparations monitored by Coulter sizing produced the expected results in mixed lymphocyte culture (MLC) testing. That is, T cells responded but did not stimulate whereas B lymphocytes and monocytes stimulated but did not respond. NULL lymphocytes both stimulated and responded. In antibody-dependent cell-mediated cytotoxicity (ADCC), null lymphocytes and monocytes functioned as effectors, whereas T lymphocytes and granulocytes did not. B lymphocyte preparations were intermediate in activity. Coulter sizing is a rapid, simple means of assessing the purity of suspensions enriched for T, B, and null lymphocytes.

Animals↗
PubMed1977

Reduction of error rates in the microlymphocytotoxicity test.

The overall error rate of the microlymphocytotoxicity test has been reduced from 1.08% in 1971 to approximately 0.35%, based on comparisons of 882 replicate test pairs (different bleedings) and on family studies, with 0.11% attributable to "reading" errors. This reduction was attributed to automation for lymphocyte adding, better incubation temperature control, better technical preparation of cells and stronger, more monospecific sera. Three types of errors contribute to the overall error rate: random, sera-related (affected by strength and specificity), and lymphocyte-related (possible B-cell reactivity and donor/lymphocyte physiological condition). Strongly skewed error distributions, which seem to be lymphocyte-related, occurred in less than 4% of the test pairs.

Cytotoxicity Tests, Immunologic↗
PubMed1974

Photochemical activity of single chloroplasts recorded by the use of nuclear track emulsion.

The photochemistry done by single chloroplasts can be measured when the chloroplasts are embedded in nuclear track emulsion. It has been known for more than 50 years that certain chemicals will blacken photographic plates (chemical fogging). Although this effect has been little used to measure chemical reactions, it may be particulary useful in photochemistry and electrochemistry, since as little as 10(-18) mole can be measured.

Chloroplasts↗
PubMedDate not supplied

Childhood-onset depressive disorders. A follow-up study of rates of rehospitalization and out-of-home placement among child psychiatric inpatients.

This report describes preliminary outcome data for a sample of child psychiatric inpatients with diagnoses of major depression and/or dysthymic disorder at the time of their hospitalizations. Depressed children were compared with a contrast group of children with schizophrenia spectrum disorders. Results (based on semi-structured telephone interviews) indicate high rates of rehospitalization among our depressed cohort. Depressed children had rehospitalization rates of 35% and 45% respectively in the first and second years after discharge. Out-of-home placement was rarer in the depressed group, and significantly less likely than for children with schizophrenia spectrum disorders. However, 15% of the depressed cohort were placed out of their homes within the first year of discharge. There were no differences between children with major depressive and dysthymic disorders on these outcome variables, underscoring the serious long-term correlates of childhood dysthymic as well as major depressive disorders.

Antidepressive Agents↗
PubMedDate not supplied

Ninety-five percent probability of paternity with HLA, ABO and haptoglobins.

Using HLA tissue typing as the basic genetic test along with ABO and haptoglobin typing, it is generally possible either to exclude a putative father conclusively or, in nonexclusion cases, to assign a greater than 95% probability of paternity. In 22 of 53 nonexclusion cases, the putative father had a probability of paternity above 99% based on these genetic tests.

ABO Blood-Group System↗
PubMedDate not supplied

Raf-1 kinase activity predicts for paclitaxel resistance in TP53mut, but not TP53wt human ovarian cancer cells.

We have recently reported that there is a significant Raf-1 kinase dependency of paclitaxel resistance in human cervical tumor cell lines. In light of the possibility that Raf-1 kinase inhibitors could be used to enhance paclitaxel responsiveness in ovarian cancer, we have characterized the Raf-1 kinase dependency of paclitaxel resistance in ovarian cancer cells. The relationship between Raf-1 kinase activity and the sensitivity to clinically relevant paclitaxel concentrations was determined in four ovarian cancer cell lines (CA-OV3, SK-OV3, 2780/WT and OAW42/WT). Furthermore, in recognition that such a drug combination would initially be used in patients whose tumors have recurred following cisplatin/paclitaxel treatment, we also determined the Raf-1 kinase dependency of paclitaxel cytotoxicity in cisplatin resistant variants of two of the ovarian cell lines (2780/CP and OAW42/CP). In the two cell lines (2780/WT and OAW42/WT) that possess a wild-type TP53 (TP53wt), the relationship between Raf-1 kinase activity and paclitaxel resistance was different from that observed in the cervical tumor cell lines. In these cell lines, paclitaxel-induced far more cell killing than would have been predicted from their Raf-1 kinase activity. However, in the ovarian cancer cell lines (CA-OV3, SK-OV3, 2780/CP and OAW42/CP) that have a mutant TP53 (TP53mut), the cytotoxicity induced by 60 nM paclitaxel exhibited the same relationship to Raf-1 kinase activity as previously observed in cervical tumor cell lines. These data suggest that the therapeutic efficacy of paclitaxel in ovarian cancer patient whose tumors have TP53mut might be increased if it is administered in combination with Raf-1 kinase inhibitors, e.g., ISIS 5132.

Antineoplastic Agents↗