[Decalcification of histological specimens].
Authors studied the effect of various decalcifying solutions on the staining of cell nuclei. It remained normal within the period necessary for decalcification only when n-hydrochloric acid containing 2 per cent of ferric chloride or solution of EDTA III were used. Decalcification of solid bone specimens in hydrochloride acid-ferric chloride solution at room temperature can be completed in 6-7 days. The staining of cell nuclei remains normal by the 12th day. A satisfactory effect--decalcification in 14 days without any damage to the cell nuclei staining--can be achieved by a 27 per cent, pH 7.4 solution of EDTA III at a temperature 60 degrees C.
Decalcification Technique↗