The terminal step of the de novo synthesis of diacyl and alkylacyl phospholipids in subfractions of rabbit sarcoplasmic reticulum ontogenesis.
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Biomedical subjects
S Pikuła
Publications and source records attributed to S Pikuła.
Potassium channel openers induce mitochondrial matrix volume changes via activation of ATP-sensitive K+ channel.
Recently, using patch-clamp method, an ATP-sensitive potassium channel in rat liver mitochondrial inner membrane was identified. In this report we investigated the effects of different potassium channel openers on potassium ion permeability of rat liver mitochondria. For that purpose we employed light scattering technique, reflecting mitochondrial volume changes linked to solute transport. Among various potassium channel openers RP 66471, pinacidil, minoxidil sulfate and KRN 2391 were found to stimulate mitochondrial swelling with high efficiency. P1060, aprykalim and diazoxide had only a slight effect, while Ro 31-6930 and nicorandil were found ineffective. Activation by the K+ openers was abolished by the antidiabetic sulfonylurea, glibenclamide (the well known blocker of ATP-sensitive potassium channels). These findings may suggest that mitochondrial ATP-dependent K+ channel is involved in regulation of mitochondrial matrix volume.
Adenosine Triphosphate↗
Participation of the multispecific organic anion transporter in hepatobiliary excretion of glutathione S-conjugates, drugs and other xenobiotics.
Polarized liver cells, hepatocytes, are involved in carbohydrate, protein and fat metabolism, breakdown of hemoglobin and production of bile. They are also involved in overall detoxification processes in an organism associated with the transport of bile salts, cholesterol, phospholipids, endo- and xenobiotics, end-products of cellular metabolism and ions through the canalicular region of the hepatocyte plasma membrane. Uptake of the above-mentioned compounds into hepatocytes through the basolateral region of plasma membrane is followed by their chemical modification by enzymes of detoxification phase I (e.g. cytochromes P-450) and phase II (e.g. glutathione S-transferases). Canalicular transport participates in phase III of detoxification, and the molecular machinery involved in this process is localized in the canalicular region of the plasma membrane. Canalicular transport includes the following transport systems: a specific canalicular transporter for bile salts, a multidrug resistance 2 P-glycoprotein (MDR2) participating in the transport of lipids, a multidrug resistance 3 P-glycoprotein (MDR3) responsible for the transport of organic cations and the multispecific organic anion transporter (cMOAT) involved in the transport of non-bile acid organic anions. The cMOAT transport system is discussed in this detailed review.
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