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S Ploeger

Publications and source records attributed to S Ploeger.

8 recordsLinked to original sources
PubMed2002

Morphometry of human superficial dorsal and dorsolateral column fibres: significance to spinal cord stimulation.

In spinal cord stimulation (SCS) large diameter cutaneous (Abeta) fibres in the dorsal columns (DCs) are activated and have an inhibiting effect on the transmission of pain signals by Adelta and C fibres from the corresponding dermatome(s). The largest Abeta fibres can be activated up to a maximum depth of about 0.25 mm in the DCs. No data are available on the distribution of the large fibres in this superficial human DC layer at the common SCS levels Th(10-11). Such data are indispensable to improve the predictive capability of a computer model of SCS. The whole myelinated fibre population in the superficial 300 microm of the dorsal column (DC(0-300)) at Th(10-11 )of two human subjects was morphometrically analysed. Some data was obtained from a third subject. The superficial dorsolateral column (DLC(0-300)) was included in this analysis because it was hypothesized that large dorsal spinocerebellar tract fibres could also be activated by SCS. Only very few fibres larger than 10.7 microm were found: a mean of 68 (0.5%) in DC(0-300) and 114 (2%) in DLC(0-300). Considering that the effect of SCS is primarily attributed to activation of these largest fibres, it is concluded that a surprisingly small average amount of 2.4 fibres per running 0.1 mm width and 6 fibres per segmental division of the DC is involved. Distinct mediolateral heterogeneity in fibre composition was found in both DC(0-300) and DLC(0-300). In the DC(0-300), the mean diameter of fibres > or =7.1 microm increases significantly by 5% from medial to lateral. Density (i.e. number of fibres per 1000 microm(2)) and frequency (i.e. percentage of a fibre size group compared to its parent population) of the large fibres increase significantly from medial to lateral in the DC(0-300). For fibres > or =10.7 microm, these parameters increase by 200 and 269%, respectively. It is concluded that the difference in stimulation threshold of large Abeta fibres in the median and lateral DC can be mainly attributed to the absence and presence, respectively, of collaterals at the stimulation site. Marked differences were found between DC(0-300) and DLC(0-300). The largest DLC(0-300) fibres (> or =10.7 microm) have a 320% higher frequency and a 473% higher density. Their mean diameter is, however, only 2% larger. The largest DLC(0-300) fibres are not likely to be recruited by SCS, since they are not larger than their DC(0-300) counterparts, they lack collaterals (which would reduce the threshold stimulus substantially) and they are more remote from the stimulation electrode.

Adult↗
PubMed1998

Preservation and staining of myelinated nerve fibers.

Six procedures are given for preservation of myelinated nerve fibers for light or electron microscopic studies. These procedures fall into two main categories: those with and those without aldehyde fixation. Essentially different effects are attained by application of tannic acid, saline, microwave or conventional heating, or a decreased temperature. All procedures end in osmication. Three main aspects of myelinated fiber morphology are taken into account when judging the quality of their preservation: axon, myelin sheath, and axon/myelin coherence. Each aspect can be preserved excellently, but always in combination with a less superior quality of the other two aspects. Superior myelin quality is attained using microwave irradiation, either with aldehydes to which tannic acid is added or without aldehyde fixatives. Superior axon quality is attained with aldehydes and (conventional) heating. Axon/myelin coherence is best preserved by decreasing the temperature during the rinse with saline. Another two procedures provide good, though less superior, preservation of both axon and axon/myelin coherence. Next, the fixed tissue is embedded in plastic blocks from which semithin and ultrathin plastic sections are cut for light and electron microscopy, respectively. In addition to the standard procedure for toluidine blue staining on semithin sections, two microwave-supported procedures are described, which can be used as alternatives if the staining result is unsatisfactory. Furthermore, a toluidine blue staining procedure is described for glycol methacrylate (GMA)-embedded material, which can be used if larger sections are needed.

Aldehydes↗
PubMed1994

Preservation of myelinated fibers for electron microscopy: a qualitative comparison of aldehyde fixation, microwave stabilisation and other procedures all completed by osmication.

A qualitative comparison was made of a variety of electron microscopic preservation methods for nervous tissue, especially with respect to myelinated fiber areas. The methods studied were aldehyde perfusion/immersion fixation, aldehyde-tannic acid immersion fixation (stimulated by either microwave or conventional heating), microwave stabilisation, saline treatment with conventional heating (all with secondary osmication), and primary osmication. For all methods three morphological aspects, the ultrastructural quality of myelin sheath and axon and the coherence between the two were judged separately. It appears that the best version of each method studied is capable of providing a good overall ultrastructural result but always shows a preference for one or two of the three separate morphological aspects. When aiming at good axon quality together with good axon/myelin coherence, aldehyde perfusion/immersion, saline treatment or primary osmication are almost equivalent. Microwave stabilisation, on the other hand, can be chosen when good myelin quality has to be combined with good axon quality. For more specific purposes the following examples can be given. When excellent myelin quality is needed both microwave-stimulated aldehyde-tannic acid fixation or microwave stabilisation can be considered. When the preservation of the axon quality has priority the aldehyde-perfused tissue should be further immersed in a heated aldehyde-tannic acid solution. Primary osmication guarantees excellent axon/myelin coherence. Despite the differences in detail, a remarkable correspondence is stressed between the overall results of sometimes extremely different methods of tissue preservation. Probably they all guarantee a reliable reflection of the in vivo situation. With respect to the use of microwave irradiation for tissue preservation, it appeared that stabilisation procedures are rather capricious. However, if successful, the results are not inferior to those of aldehyde fixation.

Aldehydes↗
PubMed1993

Does microwave irradiation have other than thermal effects on glutaraldehyde crosslinking of collagen?

The effects of microwave irradiation (MWI) can theoretically be divided in thermal and non-thermal effects. Because there is still much debate on the contribution of non-thermal microwave effects, experiments were carried out in which the effects of isothermal microwave irradiation on glutaraldehyde (GA) crosslinking of native collagen membranes (NCM) is studied. A total of 20 strips of collagen membrane of porcine origin were isothermally irradiated in 300 ml of a 0.1 (w/w) GA solution, 10 strips at 4 degrees C, and 10 strips at 20 degrees C. A temperature controlled microwave oven (630W) was adapted to allow for simultaneous cooling of the fixation solution preventing the MWI to induce any measurable thermal effects. Any possible temperature variations were recorded employing continuous fiberoptic temperature measurement. Control experiments were carried out under the same (isothermal) fixation conditions, but without MWI. The crosslinking action of the GA was evaluated by determining the shrinkage temperature (Ts) of the collagen strips. No significant difference (p > 0.05) could be observed between the increase in (Ts) of the microwaved and the increase in Ts of the nonmicrowaved strips. The increase in Ts at 4 degrees C (c. 14 degrees C) was lower than that obtained at 20 degrees C (c. 16 degrees C). It was concluded that MWI induces no substantial nonthermal effect on enhancement of GA crosslinking of collagen at these temperatures.

Animals↗
PubMed1992

Does microwave irradiation have other than thermal effects on histological staining of the mammalian CNS? A light microscopical study of microwave stimulated staining under isothermal conditions in man and rat.

The question whether or not microwave irradiation exerts other than thermal effects on histological staining is still a matter of controversy. The present study was undertaken to reveal or reject such a so far hypothetical non-thermal irradiation effect. A device was developed, which enables exposure of histological sections or tissue pieces to microwave irradiation under isothermal conditions, i.e. with synchronous removal of the internal heat produced. Three classical neuroanatomical staining methods were tested on human and rat CNS. As control, identical procedures were performed without simultaneous microwave irradiation. The experiments were performed at three different temperature levels ranging from 5 to 50 degrees C. In none of the cases studied was a light microscopically appreciable difference observed between the microwave and non-microwave versions of a stain at the same temperature. The hypothesis of a separate non-thermal effect of microwave irradiation on histological staining is therefore rejected.

Animals↗
PubMed1991

Microwave applications in classical staining methods in formalin-fixed human brain tissue: a comparison between heating with microwave and conventional ovens.

The quality of microwave adaptations of three classical neuroanatomical staining methods (the Nissl, Klüver-Barrera and Häggqvist stains) was tested on frozen serial sections from human brain specimens which has been stored for up to 10 years in 10% formalin. The conclusion was that the use of microwave irradiation reduces processing time and/or concentrations of the chemicals used, whereas the light microscopical quality of the stains considered is equal or improved as compared to their original counterparts. Next, a comparison was made between microwave adapted stains and classical procedures, which, except for the use of a conventional oven as heat source together with pre-heated solutions, were entirely identical. It appeared, that at light microscopical level no difference can be appreciated between the effect of internally (using microwave irradiation) and externally (using a conventional oven) supplied heat on the staining result.

Brain↗