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Biomedical subjects

S Prasad

Publications and source records attributed to S Prasad.

At least 235 records · Page 13Linked to original sources
PubMed1987

Dexamethasone-induced phosphorylation of high mobility group nonhistone proteins of aging rats.

Phosphorylation of high mobility group (HMG) proteins and its modulation by dexamethasone were examined in vitro by incubating liver slices of young (15-) and old (138-week) male rats with (32P) orthophosphate. HMG proteins were extracted and analyzed by acid-urea polyacrylamide gel electrophoresis. Phosphorylation of HMG proteins, particularly of HMG2, 14 and 17 decreases drastically in old rats. Dexamethasone stimulates the phosphorylation of total HMG proteins in both ages. Individual HMG proteins vary in the extent of 32P incorporation. Such differential phosphorylation of HMG proteins and its modulation by dexamethasone may affect chromatin organization and gene expression during aging.

Age Factors↗
PubMed1987

The uses of the SEPA flap in the repair of defects in the hands and fingers.

A series of 30 cases is presented in which the SEPA flap was used to provide skin cover for injuries and defects in the hand and fingers. Our experience with both unilateral and bilateral SEPA flaps confirms their reliability, convenience and versatility. The SEPA flap design can be used in conjunction with other flaps and in itself contributes to a more rational use of the skin of the lower abdomen in reconstructive surgery.

Abdomen↗
PubMed1987

Studies of the role of B-cells in the resistance of mice to experimental candidiasis.

The role of humoral immunity in experimental candidiasis was studied in B-cell-deficient mice. The B-cell deficiency was produced by administration of anti-mu antibodies to newborn animals. These anti-IgM-treated mice failed to produce antibodies against sheep red blood cells (SRBC), and their lymph nodes and spleens did not form germinal centres. However, their cell-mediated immune responses were normal as they developed delayed hypersensitivity to SRBC and rejected skin allografts like normal animals. These B-cell-deficient animals along with the controls were infected with one LD50 dose of Candida albicans. These animals were observed for mortality, antibody formation, delayed type hypersensitivity (DTH) to Candida antigen and viable count of the organism in different organs. The course of the experimental infection was almost parallel in both the groups. B-cell deficiency did not increase the susceptibility of the animals to experimental candidiasis.

Animals↗
PubMed1987

Studies of experimental candidiasis in T-cell-deficient mice.

The role of cell-mediated immunity (CMI) in the protection against experimental murine candidiasis was studied. CMI-deficient animals prepared by adult thymectomy and treatment with antimouse thymocyte serum along with controls were challenged with one LD50 (6.6 X 10(4)) dose of Candida albicans. The course of infection in these animals was studied by mortality pattern, delayed type hypersensitivity reaction (DTH) to candida antigen, antibody formation, and viable count of organisms in kidney, spleen and liver at different time intervals. The T-cell-deficient animals were found to be highly susceptible to infection with C. albicans. A correlation was observed in the appearance of DTH and protection.

Animals↗
PubMed1987

High density lipoprotein2. Relationship of the plasma levels of this lipoprotein species to its composition, to the magnitude of postprandial lipemia, and to the activities of lipoprotein lipase and hepatic lipase.

Lipoprotein lipase (LPL) activity in postheparin plasma of 38 normolipidemic volunteers was related to the magnitude of postprandial lipemia after a fat meal, to triglyceride content of high density lipoprotein2 (HDL2), to hepatic lipase (HL) activity, and to HDL2 levels. LPL activity correlated indirectly with lipemia, triglyceride content of HDL2, HL activity, and levels of HDL2 but not of HDL3. HL activity correlated directly with lipemia and indirectly with HDL2 levels. Triglyceride content of HDL2 correlated directly with lipemia and indirectly with HDL2 levels. In HDL2, abundance of apolipoprotein (apo) A-II and the apoA-I/apoA-II ratio varied widely. The latter correlated positively with LPL activity and HDL2 levels, and, inversely, with HL activity, lipemia, and triglyceride content of HDL2. The study suggests that HDL-cholesterol is not an independent parameter of lipid transport, but is strongly affected by triglyceride metabolism through lipolytic enzymes, as exemplified by postprandial lipemia that affect both composition and plasma levels of HDL2.

Apolipoproteins A↗
PubMed1986

Epithelial and basement membrane responses to chick embryo primitive streak grafts.

Chick embryo primitive streak grafts, placed beneath the epiblast of host embryos, tend to result in the formation of either a neural plate in response to anterior streak grafts, or in de-epithelialization in response to posterior grafts. Ultrastructural and immunocytochemical examination shows that both reactions are preceded by basement membrane disruption and early removal of fibronectin therefrom. This disruption does not occur in response to non-streak grafts. It is suggested that the disruption, evoked by primitive streak cells, is a prerequisite first step, allowing direct graft-epiblast cell contact. This contact elicits a specific cytoskeletal reaction determining the epiblast response.

Animals↗
PubMed1986

Insidious erosion of a digit by a ring.

Avulsion injuries in fingers can be caused by rings worn on fingers. If the ring is tight, it can also cause an unusual problem, that of deep erosion into the finger. Only two cases with such erosion, both of them in adults have been reported so far. A child with a similar problem is presented here as a case report.

Child↗
PubMed1986

[Degradation of the insecticide parathion in soil].

Degradation of the insecticide Parathion in four types of soil from the Northeast of Brazil has been studied using radiometric techniques. In all the soil samples, sterilized or not, metabolites were detected with Rf values of 0.00 and 0.17 respectively--Silica Gel 60 F254 tlc plates (Merck); solvent system: hexane-acetone (4:1, v/v)--. However, 14CO2 was formed only with the non-sterilized soils and this was independent of the use or not of organic fertilizer. These results suggest that the two metabolites, mentioned above, are formed by a predominantly non-biological pathway, where as the formation of carbon dioxide requires the participation of the microbial flora of the soil. Analysis of the interaction of 14C-Parathion with soils shows that the extent of degradation of this insecticide is modified by adsorption on the soil particles.

Adsorption↗
PubMed1985

A manoeuvre to cover exposed metal using a de-epithelialised "turn-over" fasciocutaneous flap.

This paper describes a technical manoeuvre to accommodate an exposed metal implant used in the treatment of a fracture of the tibia within a "turn-over" de-epithelialised fasciocutaneous flap. The need for such a procedure in this particular case is commented upon. A discussion is also included on the nature of the blood supply to the integument based on available information.

Adolescent↗
PubMed1985

Evaluation of intramuscular sulprostone and vacuum aspiration for termination of early pregnancy.

The results of a comparative study of the efficacy and acceptability of sulprostone administered intramuscularly and vacuum aspiration for termination of very early pregnancy is reported. The period of amenorrhoea varied from 35 to 45 days with a positive pregnancy test. Group A (20 cases) received intramuscular sulprostone in doses of 500 micrograms and 1000 micrograms 4 hours apart without any pre-treatment. The success rate was 85%. Group B (20 cases) underwent vacuum aspiration with 100% success rate. Incidence of vomiting was comparable in both groups. Severe abdominal cramps/pain occurred in 11% following sulprostone administration. No immediate serious complication occurred. Estimation of serum HCG was done before sulprostone administration and 2 weeks later. A decline was noted 2 weeks post-treatment in successful cases, thus confirming the effectiveness of the therapy.

Abortifacient Agents, Nonsteroidal↗