Biomedical subjects

S Pritlove-Carson

Publications and source records attributed to S Pritlove-Carson.

5 recordsLinked to original sources

PubMed1997

Cytokeratin phenotypes at the dento-gingival junction in relative health and inflammation, in smokers and nonsmokers.

OBJECTIVES: The cells of the junctional epithelium (JE) provide and maintain the epithelial attachment, and remain morphologically and phenotypically distinct from oral sulcular (OSE) and external oral epithelia (EOE), from which they may be regenerated de novo. Expression of cytokeratins (CK) in human epithelia has been shown to be highly site-specific, implying a functional role. The aims of this study were to differentiate between the cytokeratin profiles of JE, OSE, EOE and pocket epithelia (PE) in health and disease, in smokers and non-smokers. MATERIALS AND METHODS: The cytokeratin profiles of 40 samples of healthy and clinically inflamed human gingival tissue taken from 15 smokers and 25 non-smokers were studied by immunocytochemistry. Cryostat sections of fresh frozen gingival tissues were stained with a panel of monoclonal antibodies (mAb) and visualised by a biotin-Streptavidin-peroxidase complex technique. RESULTS: JE and PE expressed an identical range of cytokeratins irrespective of the inflammatory or smoking status, with the exception of CK4 expression, which tended to be increased in smokers. The OSE and EOE expressed non-cornifying and cornifying differentiation cytokeratins respectively, but in the presence of inflammation, both these epithelia showed increased expression of CK19 at a basal level in association with expression of one or more of the simple cytokeratins. JE/PE expressed CK17 in external layers only, approximating the tooth surface. All epithelia expressed CK6, 16 the markers of high cell turnover. CONCLUSIONS: CK 19 was a consistent differentiation marker for JE and PE. Expression of CK8, 18 was enhanced by inflammation. CK4 expression increased in association with smoking. Markers of differentiation were not always co-expressed equally within a pair. Pairs were not always completely mutually exclusive with frequent co-localisation.

Adolescent↗

PubMed1995

Evaluation of guided tissue regeneration in the treatment of paired periodontal defects.

The aim of the present study was to compare guided tissue regeneration with conventional surgery in matched periodontal defects within the same subject. Twenty pairs of sites in nine subjects were treated. Surgery was performed at both test and control sites on the same visit. Random allocation was not performed until all preparation and root instrumentation had been completed. Test defects were covered with e-PTFE membranes (Gore-Tex) and secured with sutures. Membranes were removed after 5-6 weeks with a second surgical procedure, whereas the control sites were not re-operated. At 12 months probing depth reductions were significantly greater at the Gore-Tex treated sites (P<0.05) but no difference in probing attachment level gains were found when compared with conventional flap surgery.

Adult↗

PubMed1994

Immunohistochemical analysis of tissues regenerated from within periodontal defects treated with expanded polytetrafluoroethylene membranes.

Immunocytochemical analysis was carried out on samples of 5-, 6-, and 9-week old regenerated soft tissue taken from healing periodontal defects treated by guided tissue regeneration using expanded polytetrafluorethylene (ePTFE) membranes. A panel of monoclonal and polyconal antibodies to cytokeratins, vimentin, and collagen was used to label cells and collagen types I, III, and IV. Epithelium was identified in 7 of the 9 samples examined, in addition to mesenchymal cells staining positively for vimentin and co-distribution of collagen types I, III, and IV in all samples. Clinical observations indicated that exposure of the ePTFE membranes during healing was a frequent occurrence, and the presence and quantity of epithelium found within the healing defect beneath the membrane may be related to the extent to which this occurs.

Adult↗

PubMed1992

Immunohistochemical analysis of cells attached to teflon membranes following guided tissue regeneration.

A wide panel of monoclonal antibodies to various keratins together with an anti-vimentin antibody to label mesenchymal cells were used to label cells attached to expanded polytetrafluorethylene (ePTFE) membranes removed 6 weeks postoperatively from 7 patients. All membranes demonstrated variable amounts of adherent tissue, within which vimentin-positive cells were detected. In 8 of the 18 samples, no epithelial cells were detected. Seven of the 18 samples demonstrated small groups of epithelial cells on both sides of the membranes, staining predominantly as basal cells. The remaining 3 samples were more densely populated by epithelium which expressed a diverse keratin profile. Thus, the membranes were successful in establishing mesenchymal cell proliferation, as evidenced by vimentin positivity, but did not totally exclude epithelium.

Antibodies, Monoclonal↗

PubMed1992

Guided tissue regeneration--why, when and how?

Chronic periodontitis results in the destruction of the collagen fibre attachment to cementum, apical migration of the epithelial attachment, contamination of the exposed root surface by plaque and loss of alveolar bone. Regeneration can be defined as the restitution of lost tissues to a state which existed before destruction. As such, it is an ultimate and ideal goal and in most circumstances is currently beyond our capabilities. However, research in periodontology over the last 10 years or so has made considerable advances and is perhaps unique in proposing a new and fundamental treatment modality--guided tissue regeneration.

Alveolar Bone Loss↗