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Biomedical subjects

S R Deshmukh

Publications and source records attributed to S R Deshmukh.

5 recordsLinked to original sources

Norfloxacin induced resistance to fluoroquinolones & structurally unrelated antimicrobial agents in coagulase negative staphylococci.

Nine clinical isolates of coagulase negative staphylococci (CONS) susceptible to norfloxacin (MIC 1.8-2 micrograms/ml) were manipulated in vitro to induce norfloxacin resistance by means of serial passage in brain heart infusion broth containing increasing concentrations of norfloxacin. Exposure of CONS to norfloxacin resulted in 18 to 20 times increase in MIC of norfloxacin and change in in vitro susceptibility to ciprofloxacin, pefloxacin, ofloxacin, kanamycin, neomycin and tobramycin, indicating development of cross resistance to fluoroquinolones and aminoglycosides. These results show that exposure to increasing concentrations of norfloxacin can induce the development of resistance to various antimicrobial agents, suggesting its mutagenic role.

Anti-Infective Agents

A comparison of sputum examination for acid fast bacilli by modified Schaeffer & Fulton stain, Ziehl-Neelsen stain & cold stain.

A comparative study of Ziehl-Neelsen stain, cold stain and modified Schaeffer and Fulton stain was carried out to evaluate the efficiency of modified Schaeffer and Fulton method in sputum examination for acid fast bacilli (AFB). Of 187 sputum samples studied, 67 (35.82%) were reported positive by Ziehl-Neelsen stain and cold stain method while 66 (35.29%) were reported positive by modified Schaeffer and Fulton method. In comparison with Ziehl-Neelsen staining, 98.58 per cent positivity was reported by modified Schaeffer and Fulton method. Modified Schaeffer and Fulton method is found to be simple, reliable, less expensive and as efficient as Ziehl-Neelsen stain and cold stain for demonstration of acid fast bacilli in sputum.

Bacillus

The plague.

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Disease Outbreaks